The NextGen-O2k all-in-one enables the following applications:
- oxygen consumption
- Q-redox state
- NAD(P)H-redox state
- H2O2 production
- ATP production
- Ca2+
- mitochondrial membrane potential
- photosynthesis
- light-enhanced dark respiration
The NextGen-O2k all-in-one also supports all add-on Oroboros Modules: ISE TPP-Module, ISE pH-Module, NO-Module, enabling measurement of mt-membrane potential with ion sensitive electrodes (ISE for TPP+ or TPMP+ ) or pH.
This package is available as a Power Package to increase throughput. Certain auxiliary tools and services are not included in Power Packages, more details:
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For NextGen-O2k Series XA and upwards, DatLab 8 ensures proper operation and data acquisition for all Oroboros Modules.
Award-winning innovation
Configuration
NextGen-O2k: Hardware and DatLab Software*
Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 Sockets for Q-and NAD(P)H-Modules
2 Sockets for PB-Module
2 Chambers cV (2.0 mL, Duran® glass)
2 SmartPOS
2 Stirrer Bars cV (white PVDF) , electromagnetic control
2 Stoppers cV (black PEEK) with O-rings cV
2 Chamber Holders cV (blue POM) with O-ring and V-ring
2 POS Holders (blue POM)
Current DatLab version with free follow-up upgrades within the warranty period
NextGen-O2k: Accessories and Consumables
1 ISS-Integrated Suction System, to remove medium from the Chamber
1 Oxia – the O2 regime controller for HyperOxia and HypOxia
1 Titration Set: Hamilton microsyringes; fixed needles with rounded tips (8 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 2 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 Pkg. POS Seal Tip
2 Pkg. POS Membranes
1 POS Electrolyte Powder
1 POS Mounting Tool
1 Polishing Cloth
1 Polishing Powder 0.3 μm
1 O2-Zero Powder
1 Forceps for membrane application
1 Pipette Plastic ungraded
1 Pkg. Conditioning cloths for stainless-steel housing
Q-Module: for measuring the Q-redox state and cyclic voltammetry
4 Q-Stoppers cV (beige PEEK \ conical shaft \ side+2.6 mm +1.3 mm central port): with glassy carbon and platinum electrode embedded
2 Reference Electrodes, 2.4mm Ø glass shaft
10 Replacement Barrels for reference electrodes, 2.4 mm glass
1 Electrolyte for Reference Electrode
1 ISE Filling Syringe with needle
1 Stopper Needle
2 Polishing Powder 0.3 μm
2 Polishing Powder 0.05 µm
4 Polishing Cloth
3 Pkgs Q-Filter Papers (10/Pkg)
2 Boxes O-ring cV \ Viton® \ 12.5×1 mm, 8/box
NADH-Module: for simultaneous measurement of oxygen consumption and NAD(P)H autofluorescence.
2 NADH Sensors
2 Stirrer Bars cV (black PVDF)
3 Pkg of Filter Sets for NADH (12/Pkg)
PB-Module: for conducting measurements of PhotoBiology, including photosynthesis
6 PhotoBiology light sources – PB Light Sources (2 blue, 2 red and 2 white)
Fluo Smart-Module: an amperometric add-on module for measurement of hydrogen peroxide production, ATP production, mt-membrane potential, Ca2+
2 SmartFluo Sensors, green LED emission, photodiode and filters, excitation LED 525 nm, pre-calibrated intensity
2 SmartFluo Sensors, blue LED emission, photodiode and filters, excitation LED 465 nm, pre-calibrated intensity
3 Filter Sets (Mg green/Ca Green, Amplex® UltraRed, Safranin) consisting of LED filters and photodiode filters
TIP2k-Module: add-on for two-channel operation with the Oroboros with automatic control by DatLab of programmable titration regimes and feedback control
4 Micro-injection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip
1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool
1 TIP2k-Needle Safety Support with cable guide
1 Pkg. TIP2k-Filter Papers (10/Pkg.)
sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function
2 Chambers sV (Duran® glass), with smaller operation volume (V) of 0.5 mL
2 Stirrer Bars sV (white PVDF) for small volume chambers, electromagnetic control
2 Stoppers sV (black PEEK) for small volume chambers with O-ring sV
2 Chamber Holders sV (black POM) with O-ring and V-ring
2 POS Holders sV (black POM)
Included extras
Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 Installation and startup support session (remote, to be used within the warranty period)
1 Oroboros Workshop registration fee for the participation of one lab member waived (to be used within the warranty period)
3-year warranty
* Integrated computer with touch screen (15,6″; FHD 1920×1080) connects DatLab to the NextGen-O2k
* Keyboard set, webcam and webcam accessories
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The complete experimental system for high-resolution fluorespirometry, redox biology and PhotoBiology: enabling simultaneous monitoring of oxygen consumption together with coenzyme Q redox state, NAD(P)H redox state, H2O2 production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+uptake capacity (CaG), ATP production (MgG) or photobiology measurements.
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