Mitochondrial membrane potential
The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, ΔpH+.
Δψ = ΔpH+ – ΔµH+ / F
mtMP or Δψ is the potential difference across the mitochondrial (mt) inner membrane, expressed in the electrical unit volt [V].
mt-Membrane potential can either be measured in the O2k-FluoRespirometer fluorometrically by using the fluorophores TMRM, Safranin, or Rhodamine 123, or potentiometrically with the O2k-TPP+ ISE-Module, which can be applied with any O2k configuration (Series B or higher), by using the ion reporter TPP+. All mentioned ion indicator molecules are cations which inhibit respiration, making it essential carry out respiratory controls to evaluate the effect of the indicator.
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Includes the 2.0 mL O2k-Chamber and Fluo-Sensors needed for measurement of the mt-membrane potential.
Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. -»Bioblast link«
Sumbalova Z, Gnaiger E (2015) High-resolution measurement of mitochondrial membrane potential and respiration – comparison of potentiometric and fluorometric methods. Abstract MiP2015. -»Bioblast link«