Mitochondrial membrane potential measurement

Mitochondrial membrane potential

The mitochondrial membrane potential, mtMP, is the electric part of the protonmotive force, ΔpH+.

Δψ = ΔpH+ – ΔµH+ / F

mtMP or Δψ is the potential difference across the mitochondrial (mt) inner membrane, expressed in the electrical unit volt [V].

mt-Membrane potential can either be measured in the O2k-FluoRespirometer fluorometrically by using the fluorophores TMRM, Safranin, or Rhodamine 123, or potentiometrically with the O2k-TPP+ ISE-Module, which can be applied with any O2k configuration (Series B or higher), by using the ion reporter TPP+. All mentioned ion indicator molecules are cations which inhibit respiration, making it essential carry out respiratory controls to evaluate the effect of the indicator.

Meet the expert

Luiza Cardoso, PhD
PostDoc, Mitochondrial Wizard