ATP production measurement

ATP production

Adenosine triphosphate (ATP) is a nucleotid and functions as the major carrier of chemical energy in the cells. As it transfers its energy to other molecules, it loses its terminal phosphate group and becomes adenosine diphosphate (ADP).

Magnesium Green (MgG), an extrinsic fluorophore, can be applied for measurement of mitochondrial ATP production with mitochondrial preparations. This dye fluoresces when bound to Mg2+. The technique to measure mitochondrial ATP production is based on the fact that Mg2+ presents different dissociation constants for ADP and ATP, and the adenine nucleotide translocase (ANT) exchanges ATP for ADP.

Meet the expert

Luiza Cardoso, PhD
PI, Mitochondrial Wizard

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Includes the 2.0 mL O2k-Chamber and Fluo-Sensors needed for ATP production measurement.

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References

Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001

Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. -»Bioblast link«

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Sabine Schmitt, PhD
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