Hydrogen peroxide, H2O2 or dihydrogen dioxide, is one of several reactive oxygen intermediates generally referred to as reactive oxygen species (ROS). It is formed in various enzyme-catalyzed reactions (e.g., superoxide dismutase) with the potential to damage cellular molecules and structures. H2O2 is dismutated by catalase to water and oxygen. H2O2 is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS.
H2O2 concentration can be measured in the O2k-FluoRespirometer fluorometrically by using the fluorophore Amplex UltraRed. H2O2 concentration and H2O2 production can be measured in the O2k electrochemically with the O2k-NO Amp-Module, which can be applied with any O2k configuration (Series D or higher), and an amperometric H2O2 sensor.
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Includes the 2.0 mL O2k-Chamber and Fluo-Sensors needed for H2O2 production measurement.
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