Hydrogen peroxide production measurement

H2O2 production

Hydrogen peroxide, H2O2 or dihydrogen dioxide, is one of several reactive oxygen intermediates generally referred to as reactive oxygen species (ROS). It is formed in various enzyme-catalyzed reactions (e.g., superoxide dismutase) with the potential to damage cellular molecules and structures. H2O2 is dismutated by catalase to water and oxygen. H2O2 is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS.

H2O2 concentration can be measured in the NextGen-O2k Fluo and O2k-Fluo fluorometrically by using the fluorophore Amplex UltraRed. H2O2 concentration and H2O2 production can be measured in the NextGen-O2k and O2k electrochemically with the O2k-NO Amp-Module, which can be applied with O2k configurations O2k-Series D – I and NextGen-O2k-Series XA or higher, and an amperometric H2O2 sensor.

Meet the expert

Sabine Schmitt, PhD
PI, Mitochondrial Detective


H2O2 measurements are possible with the following O2k-Products:


Bioblast"" resources


Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004

Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. -»Bioblast link«

Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. -»Bioblast link«

Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. -»Bioblast link«



More O2k - H2O2 experts

Luiza Cardoso, PhD
PI, Mitochondrial Wizard

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