Hydrogen peroxide production measurement

H2O2 production

Hydrogen peroxide, H2O2 or dihydrogen dioxide, is one of several reactive oxygen intermediates generally referred to as reactive oxygen species (ROS). It is formed in various enzyme-catalyzed reactions (e.g., superoxide dismutase) with the potential to damage cellular molecules and structures. H2O2 is dismutated by catalase to water and oxygen. H2O2 is produced as a signaling molecule in aerobic metabolism and passes membranes more easily compared to other ROS.

H2O2 concentration can be measured in the O2k-FluoRespirometer fluorometrically by using the fluorophore Amplex UltraRed. H2O2 concentration and H2O2 production can be measured in the O2k electrochemically with the O2k-NO Amp-Module, which can be applied with any O2k configuration (Series D or higher), and an amperometric H2O2 sensor.

Meet the expert

Tímea Komlódi, PhD
PostDoc, Mitochondrial Explorer

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Includes the 2.0 mL O2k-Chamber and Fluo-Sensors needed for H2O2 production measurement.

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