Ca2+ concentration measurement

Ca2+ concentration

Ca2+ is a major signaling molecule in both prokaryotes and eukaryotes. Its cytoplasmic concentration is tightly regulated by transporters in the plasma membrane and in the membranes of various organelles. For this purpose, it is either extruded from the cell through exchangers and pumps or stored in organelles such as the endoplasmic reticulum and the mitochondria. Changes in the concentration of the cation regulate numerous enzymes including many involved in ATP utilizing and in ATP generating pathways and thus ultimately control metabolic activity of mitochondria and the entire cell. Measuring changes in Ca2+ levels is thus of considerable interest in the context of high-resolution respirometry.

Ca2+ concentration can either be measured in the O2k-FluoRespirometer by using the fluorophore Calcium Green or potentiometrically with the O2k-TPP+ ISE-Module, which can be applied with any O2k configuration (Series B or higher), by applying Ca2+ membranes.

Meet the expert

Cristiane Cecatto, PhD
Mitochondrial Phoenix

Recommended O2k-Products

Includes the 2.0 mL O2k-Chamber and Fluo-Sensors needed for Ca2+ concentration measurement.

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References

Nászai A, Terhes E, Kaszaki J, Boros M, Juhász L (2019) Ca(2+)N it be measured? Detection of extramitochondrial calcium movement with high-resolution FluoRespirometry. Sci Rep 9:19229. -»Bioblast link«

Elustondo PA, Negoda A, Kane CL, Kane DA, Pavlov EV (2014) Spermine selectively inhibits high-conductance, but not low-conductance calcium-induced permeability transition pore. Biochim Biophys Acta 1847:231–40. -»Bioblast link«

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