Workshops are offered on a regular basis in Austria and in other countries in cooperation with Oroboros Network Labs. Content can vary from basic information to module-specific techniques.
Workshop: HRR and the Oroboros
The Workshop on high-resolution respirometry provides a detailed introduction to the Oroboros, overview presentations on high-resolution respirometry (HRR), and applications in mitochondrial physiology.
The Workshop programme is complemented by lectures on mitochondrial physiology (general topics; Oroboros Procedures). Short presentations of ‘Oroboros perspectives’ provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.
Workshop: Advanced Oroboros FluoRespirometry
The Advanced Oroboros FluoRespirometry Workshop is focused on fluorometry combined with respirometry, including information on FluoRespirometry, with resolution of hydrogen peroxide in the nanomolar range. High-resolution respirometry (HRR) is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production (Amplex® UltraRed), mt-membrane potential (Safranin), ATP production (Magnesium Green™), and Ca2+ (Calcium Green™). Further Oroboros MultiSensor applications are covered in these specific O2k-Workshops.
Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of ‘Oroboros perspectives’.
Workshop: TPP/ ISE
The TPP/ISE Workshop is customized for participants interested in Oroboros MultiSensor applications, particularly ion-selective electrodes (ISE) for measurement of mitochondrial membrane potential (TPP+, TPMP+) and Ca2+. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) constitutes a major topic.
According to the interest of participants, other Oroboros MultiSensor applications can be discussed and demonstrated:
- Measurement of Ca2+, calibration and calculation of free Ca2+ in buffered solutions (TPP ISE-Module)
- Acidification rate (pH)
- FluoRespirometry
- Nitric Oxide (NO)
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