Oroboros Workshops

Oroboros Workshops have been organized as international oxygraph courses (IOC) on high-resolution respirometry since 1991. These range from four full-day training courses to conference lunch presentations of the Oroboros.

Workshops are offered on a regular basis in Austria and in other countries in cooperation with Oroboros Network Labs. Content can vary from basic information to module-specific techniques.

Workshop: HRR and the Oroboros

The Workshop on high-resolution respirometry provides a detailed introduction to the Oroboros, overview presentations on high-resolution respirometry (HRR), and applications in mitochondrial physiology.

The Workshop programme is complemented by lectures on mitochondrial physiology (general topics; Oroboros Procedures). Short presentations of ‘Oroboros perspectives’ provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.

Workshop: Advanced Oroboros FluoRespirometry

The Advanced Oroboros FluoRespirometry Workshop is focused on fluorometry combined with respirometry, including information on FluoRespirometry, with resolution of hydrogen peroxide in the nanomolar range. High-resolution respirometry (HRR) is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production (Amplex® UltraRed), mt-membrane potential (Safranin), ATP production (Magnesium Green™), and Ca2+ (Calcium Green™). Further Oroboros MultiSensor applications are covered in these specific O2k-Workshops.

Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of ‘Oroboros perspectives’.

Workshop: TPP/ ISE

The TPP/ISE Workshop is customized for participants interested in Oroboros MultiSensor applications, particularly ion-selective electrodes (ISE) for measurement of mitochondrial membrane potential (TPP+, TPMP+) and Ca2+. It covers an introduction to basic concepts on mitochondrial membrane potential: proton pumps, ion gradients, electrochemical potential (proton motive force) and the measurement of mt-membrane potential in biological samples (homogenate, permeabilized fibres). Furthermore, data analysis (from TPP+ to mt-membrane potential) constitutes a major topic.

According to the interest of participants, other Oroboros MultiSensor applications can be discussed and demonstrated:

  • Measurement of Ca2+, calibration and calculation of free Ca2+ in buffered solutions (TPP ISE-Module)
  • Acidification rate (pH)
  • FluoRespirometry
  • Nitric Oxide (NO)

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Oroboros Workshops

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