O2k-TPP+ ISE-Module

Potentiometric ion-selective electrodes for measurement of mitochondrial membrane potential

Product ID: 12300-03

Description

O2k-TPP+ ISE-Module: 2 potentiometric ion-selective electrodes (ISE), supported by the Oroboros O2k (O2k-Series D and higher). The ISE module is designed with replaceable membranes making it possible to measure different ions such as TPP+ or Ca2+ with the same electrode housing*. The main application of the O2k-TPP+ ISE-Module is measurement of mitochondrial membrane potential with tetraphenylphosphonium (TPP+) or TPMP+.

*There are other ion selective membranes available (e.g., from Sigma Aldrich), for a wide variety of ionic species. If cut to a circle with a diameter of 4 mm these membranes can be used in the ISE system. Many ions may also be detected by fluorimetric based methods.

Configuration

The O2k-TPP+ ISE-Module includes:

2 Ion Selective Electrodes (ISE) 2.4mm Ø PVDF
2 Reference electrodes, 2.4mm Ø glass shaft
2 PEEK stoppers
4 Boxes TPP+ membranes, 5/box, PVC 4mm Ø
1 ISE membrane mounting tool
1 Forceps for membrane mounting
1 Filling syringe with needle
4 Replacement barrels for reference electrodes
2 Stopper-Spacer
1 TIP2k-Needle Safety Support with cable guide
Current DatLab version with free follow-up upgrades within the warranty period

Bioblast resources

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O2k-TPP+ ISE-Module