• O2k-Fluorometry and H₂O₂ production in mouse brain mitochondria. MiPNet17.17

    O2k - high-output in

    high-resolution respirometry, HRR

'OXPHOS analysis extends simple bioenergetics to the level of mitochondrial physiology' (Gnaiger 2012).

O2k

• The world's most Powerful, Popular and Prestigeous respirometer for cellular and mitochondrial research.^a
• High efficiency - low running costs.^b
• O2k-MultiSensor: Combining HRR with fluorometry (ROS production, mt-membrane potential, ATP-production, Ca²⁺), potentiometric measurement of pH, TPP⁺ and Ca²⁺, amperometric measurement of NO.^c
• World-wide standard for diagnosis of mitochondrial function in health and disease - when there is little room for error.
• Flexibility for respiration of isolated mitochondria, homogenates, cultured cells and small tissue biopsies.^d
• OXPHOS analysis with substrate-uncoupler-inhibitor titration (SUIT) protocols to study multiple respiratory states in a single experimental incubation: no other platform is suited for application of SUIT protocols.
• Flexibility within experiments, on-line display of concentration and flux, automatic software controlled titrations.^e

Reference:

Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.

Back from Everest Base Camp and Kathmandu with 5 O2k:

>> Xtreme Everest 2

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>> O2k versus XFe - why it matters

OROBOROS - MiPArt Gallery

OROBOROS - Top 10 Reasons:  Leading technology, sole source, long-term experience, economical, no investors, no expensive advertising, scientific committment, Corporate Social Responsibility ...

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Gentle Science Shapes the World   Mitochondrial Physiology Society - MiP MiP2013 Conference on Comparative Mitochondrial Physiology Sept 23-27 2013, Obergurgl, Austria

►Bioblast wiki  ►O2k-Catalogue  ►O2k-Fluorescence  ►MitoPedia Glossary: Respiratory states

    (a) >925 O2k-Publications; O2k-WorldWide

HRR since 1992 - Ref: Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.

    (b) High efficiency - low running costs

    (c) O2k-Fluorescence; O2k-TPP and Ca²⁺; O2k-pH; O2k-NO

    (d) HRR of isolated mitochondria, intact cells,  permeabilized cells, permeabilized tissues

    (e) TIP2k

Ref: Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.