High-resolution respirometry (HRR)
- O2k-Workshop: 2014 Mar 27, Spitsbergen, NO
- O2k-Workshop: HRR and O2k-Fluorometry 2014 Apr 07-12, Schroecken, Austria
- O2k-Workshop: 2014 May 01-02, La Jolla, US
- O2k-Workshop: HRR and O2k-Fluorometry 2014 Sep 12-16, Obergurgl, Austria
- O2k-Workshop: HRR and O2k-Fluorometry 2014 Oct 07-12, Schroecken, Austria
OROBOROS is also represented at a number of international events and conferences.
See OROBOROS Events for more details and take the opportunity to come and meet us at a local event.
OROBOROS O2k-Workshops have become world-famous. They are offered on a regular basis in Austria and occasionally in other countries in cooperation with OROBOROS MiPNet Reference Laboratories. O2k-Workshops are held in different formats:
In order to introduce the participants to the technical and operational details, specific emphasis is placed on hands-on practical sessions with the instrument and also with the DatLab software.
O2k-Workshop: HRR and O2k-Fluorometry
The O2k-Workshop provides a detailed introduction to the OROBOROS Oxygraph-2k (O2k-Core), overview presentations on high-resolution respirometry (HRR), and applications in mitochondrial physiology. An introduction to O2k-Fluorometry is integrated into the programme.
The O2k-Workshop programme is complemented by lectures on mitochondrial physiologoy (general topics; substrate-uncoupler-inhibitor titration SUIT protocols). Short presentations of 'Hot topics in Mitochondrial Physiology' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.
The O2k-Fluorometry Workshop is integrated into the general O2k-Workshop, introducing fluorometry combined with high-resolution respirometry (HRR). Following a short introduction to HRR, the O2k-Fluorescence LED2-Module is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin), ATP production (Mg green), and Ca2+ (Ca green).
Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of 'Hot topics in Mitochondrial Physiology'.
According to the interest of participants, further O2k-MultiSensor applications are covered in specific O2k-Workshops:
Contents O2k-Workshop: Overview on High-Resolution Respirometry
Introduction: Mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance.
High-resolution respirometry; what makes the difference? Presentation of the OROBOROS Oxygraph-2k
O2k and TIP2k: On-line instrumental performance
Polarographic oxygen sensor (POS) and O2k service
Cleaning of anode and cathode.
Electrolyte and membrane application.
O2k and TIP2k: instrumental maintenance.
* Next O2k-Workshop Basic: 2014 Apr 7-12
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
List of all O2k-Workshops
* IOC80 Schroecken, Oct 2013