OROBOROS O2k-Workshops:

High-resolution respirometry (HRR)

and O2k-Fluorometry

 



OROBOROS is also represented at a number of international events and conferences.
See OROBOROS Events for more details and take the opportunity to come and meet us at a local event.



General Information

   

OROBOROS O2k-Workshops have become world-famous. They are offered on a regular basis in Austria and occasionally in other countries in cooperation with OROBOROS MiPNet Reference Laboratories. O2k-Workshops are held in different formats: 

In order to introduce the participants to the technical and operational details, specific emphasis is placed on hands-on practical sessions with the instrument and also with the DatLab software.



Various topics



O2k-Workshop: HRR and O2k-Fluorometry

 

The O2k-Workshop provides a detailed introduction to the OROBOROS Oxygraph-2k (O2k-Core), overview presentations on high-resolution respirometry (HRR), and applications in mitochondrial physiology. An introduction to O2k-Fluorometry is integrated into the programme.

The O2k-Workshop programme is complemented by lectures on mitochondrial physiologoy (general topics; substrate-uncoupler-inhibitor titration SUIT protocols). Short presentations of 'Hot topics in Mitochondrial Physiology' provide participants with the opportunity to present and discuss their work, including projects in progress, methodological details and conceptual aspects.



O2k-Fluorometry Workshop

 

The O2k-Fluorometry Workshop is integrated into the general O2k-Workshop, introducing fluorometry combined with high-resolution respirometry (HRR). Following a short introduction to HRR, the O2k-Fluorescence LED2-Module is presented in overview lectures and hands-on sessions. Discussions cover protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin), ATP production (Mg green), and Ca2+ (Ca green).

Following our successful IOC tradition, participants are invited to submit abstracts for short presentations of 'Hot topics in Mitochondrial Physiology'.

 

According to the interest of participants, further O2k-MultiSensor applications are covered in specific O2k-Workshops:



Contents O2k-Workshop: Overview on High-Resolution Respirometry

 

Introduction: Mitochondrial and cellular respiratory physiology; new challenges for high instrumental performance.

High-resolution respirometry; what makes the difference? Presentation of the OROBOROS Oxygraph-2k

  • Optimum system design - the OROBOROS Oxygraph-2k.
  • DatLab: on-line recording of oxygen concentration and flux; linear slope versus oxygen flux as a function of time.
  • DatLab: the specialized software for high-resolution respirometry; high resolution calibrations.
  • Low oxygen and measurement of cellular oxygen consumption – pushing the limits of detection.

O2k and TIP2k: On-line instrumental performance

Polarographic oxygen sensor (POS) and O2k service

  • Cleaning of anode and cathode.
  • Electrolyte and membrane application.
  • O2k and TIP2k: instrumental maintenance.




Contact

 

O2k-Workshop: instruments@oroboros.at

 

OROBOROS INSTRUMENTS

high-resolution respirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20



List of all O2k-Workshops

* IOC80 Schroecken, Oct 2013

>> See all O2k-Workshops




 

OROBOROS O2k-Workshops 

are listed as MitoGlobal Events


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© OROBOROS High-Resolution Respirometry