In April 2010, we presented the newest developments of the O2k-MultiSensor Systemwith the OROBOROS Ion Selective Electrode (ISE) for simultaneous monitoring of respiration and TPP+.
The O2k-MultiSensor extension of the OROBOROS Oxygraph-2k yields a record of a potentiometric (voltage) signal simultaneously to the oxygen signal in both chambers of the O2k. The new ion selective electrode (ISE) system consists of separate reference and measuring electrodes. Typical examples are electrodes selective for hydrophobic cations (TPP+, TPMP+), Ca2+, Mg2+.
Figure 1: Simultaneous determination of respiratory activity (top graph) and changes in the mitochondrial membrane potential (bottom graph) in the acute lymphoblastic leukemia cell line C7H2. Cell density: 5.7∙106∙ml-1 in MiR06.