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IOC70 O2k-Workshop Barcelona
Oxygraph-2k: High-Resolution Respirometry and O2k-Fluorometry
Two-day O2k-Workshop presented by Erich Gnaiger
Date: 2012 May 29 - 30
Location: Esther Kopolovitz Center (CEK), IDIBAPS-Hospital Clinic de Barcelona, c/Rossello 153, Meeting rooms 1 and 2, 08036 Barcelona
May 29: General introduction to the Oxygraph-2k. 10:00 - 13:00 h, 15:00 - 19:00 h
May 30: The new O2k-Fluorescence LED2 module and Hot Topics in Mitochondrial Physiology. 10:00 - 13:00 h, 15:00 - 19:00 h
Limited number of Participants; Registration required. All short presentations (10+5 min) are scheduled on May 30, in the late morning and late afternoon sessions.
Registration (informal): Dr. Pablo Garcia-Roves, Esther Kopolovitz Center (CEK), Barcelona - OROBOROS MipNet Reference Laboratory
Contribution to K-Regio project MitoCom Tyrol; information from the previous O2k-Fluorometry Workshop.
IOC70 Programme 1 | Tuesday, May 29 | | | O2k-Workshop | Weblink | 09:45 | Arrival and registration | | | Principles of high-resolution respirometry - from switching on the Oxygraph-2k to the experimental result | Gnaiger 2008 POS | 10:00-10:05 | Pablo Garcia-Roves:
Welcome to the Madrid O2k-Workshop. | | 10:05-11:15 | Erich Gnaiger:
The O2k-concept on high-resolution respirometry. | Glossary: Respiratory states | | 1. Get O2k-connected with your OROBOROS-USB:
a guided tour to the O2k. | get O2k-Connected | 11:15 | Coffee / Tea - Get-together - introduction of the participants and their research interests | POS Service | 11:45-13:00 | 2. Oxygraph-2k: O2k-Setup and OroboPOS service. | O2k-Start | | 3. OroboPOS - calibration, stability testing, and evaluation of sensitivity to measure oxygen flux. | O2k-Calibration | | 4. O2k-background test and analysis of oxygen flux. | O2k-Background | 13:30 | Lunch | | 15:00-16:30 | Erich Gnaiger:
Experimental design - Substrate and coupling control
of mitochondrial respiration: SUIT protocols. | Gnaiger 2009 Int J Biochem Cell Biol | 16:30 | Coffee / Tea | CI+II | 17:00-19:00 | Pablo Garcia-Roves and Erich Gnaiger:
O2k-Demoexperiment | Pesta 2012 Methods Mol Biol | | Further information from the O2k-Manual and O2k-Protocols | | DatLab Guide through the menues:
DL-Demo files and DL-Excel templates | DatLab 4 Guide | | DatLab Analysis and Excel templates | DatLab4 Flux Analysis | | Coupling control of mitochondrial respiration: LEAK, OXPHOS, ETS, ROX | mt-Respiration | | Coupling Control Protocol with intact cells: ROUTINE, LEAK, ETS, ROX | Cell Respiration | | | | 2 | Wednesday, May 30 | | | O2k-Fluorometry and Mitochondrial Physiology | Weblink | 10:00-11:30 | Erich Gnaiger:
The O2k-Fluorometer concept | | | 1. The O2k-Fluorescence LED2-Module. | O2k-Fluorescence_LED2-Module | | 2. O2k-Fluo application with Amplex Ultrared: Oxygen dependence of hydrogen peroxide production. | O2k-Fluorescence | 11:30 | Coffee / Tea | | | Hot MiP-Topics (Chair: Pablo Garcia-Roves) | | 12:00-12:15 | Perales Jose C: | | | Do we understand hepatic gluconeogenesis? | | 12:15-12:30 | Hernandez Maribel: | | | Mitofusin 2 (Mfn2) links mitochondrial and endoplasmic reticulum function with insulin signaling and is essential for normal glucose homeostasis. | | 12:30-12:45 | Claret Marc: | | | Deletion of mitochondrial fusion protein Mitofusin 2 in hypothalamic POMC neurons leads to obesity and impaired glucose homeostasis in mice. | | 12:45-13:00 | Villena Josep A: | | | Transcriptional control of mitochondrial function in white adipose tissue by PGC-1 co-activators. | | 13:30 | Lunch | | 15:00-16:30 | Erich Gnaiger:
Permeabilized fibres vs muscle tisse homogenate | | | 1. Are permeabilized muscle fibres a valid model for measurement of hydrogen peroxide production? | | | 2. Mitochondrial respiratory function in tissue homogenate (PBI-Shredder) versus permeabilized fibres. | Shredder_vs_Fibres | | 3. Respiration with succinate - paradoxes in MiP. | IOC66.pdf | 16:30 | Coffee / Tea | | 17:00-18:00 | Erich Gnaiger: | | | Fluorometric (AmR) and potentiometric (TPP) measurement of mt-membrane potential and future perspectives O2k-MultiSensor developments. | O2k-MultiSensor | | Hot MiP-Topics (Chair: Pablo Garcia-Roves) | | 18:00-18:15 | Vieira Elaine: | | | The effect of melatonin on mitochondrial respiration. | | 18:15-18:30 | Garrabou Gloria: | | | Oxygen consumption in mitochondrial toxicity and disease. | | 18:30-18:45 | Bosch Marta: | | | Mitochondrial cholesterol: A connection between caveolin, metabolism, and disease. | | 18:45-19:00 | Summary discussion: O2k-Workshop Feedback | O2k-Feedback |
IOC70 Participants Name | Lab | Alcarraz Gema | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES | Bano Maria | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Secondary lesions due to chronic alcohol ingestion, muscle pathology group - ES | Bosch Marta | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Cell proliferation and signalling group - ES Barcelona Bosch M | Catalan Marc | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Secondary lesions due to chronic alcohol ingestion, muscle pathology group - ES | Claret Marc | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES | De Nigris Valeria | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES | Diaz-Ramos Angels | Institute for Research in Biomedicine, IRB Barcelona - ES | Garcia-Roves Pablo M. | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES Barcelona Garcia-Roves PM
| Garrabou Gloria | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Secondary lesions due to chronic alcohol ingestion, muscle pathology group - ES | Gnaiger Erich | DSL, Dept Visceral, Transpl Thoracic Surgery, Medical Univ Innsbruck - AT Innsbruck OROBOROS INSTRUMENTS | Gonzalez Franquesa Alba | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES Barcelona Garcia-Roves PM
| Herms Albert | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Cell proliferation and signalling group - ES | Hernandez-Alvarez Maribel | Institute for Research in Biomedicine, IRB Barcelona - ES Barcelona Zorzano A | Lerin Carles | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES | Llobet Sese Laura | Biochemistry and Molecular and Cellular Biology Department, Faculty of Veterinary University of Zaragoza, Laboratory Dr.Julio Montoya - ES | Martinez Laura | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES | Martinez Lourdes Navarro | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES Barcelona Garcia-Roves PM | Matias Nuria | Institute of Biomedical Research of Barcelona - ES | Mendez Andres | Bellvitge Biomedical Research Institute, IDIBELL, University of Barcelona - ES | Moren Constanza | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Secondary lesions due to chronic alcohol ingestion, muscle pathology group - ES | Perales Jose C | Bellvitge Biomedical Research Institute, IDIBELL, University of Barcelona - ES Barcelona Bermudez MJ | Ribas Vincente | Institute of Biomedical Research of Barcelona - ES | Schneeberger Marc | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES | Tobias Ester | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Secondary lesions due to chronic alcohol ingestion, muscle pathology group - ES | Vieira Elaine | August Pi i Sunyer Biomedical Research Institute, IDIBAPS Barcelona, Diabetes and obesity laboratory - ES | Villena Josep A | Vall D’Hebron Research Institute, VHIR Barcelona - ES |
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