High-Resolution Respirometry - HRR- In mitochondrial physiology and pathology
- Substrate-uncoupler-inhibitor titrations, kinetics, OXPHOS flux control
- Covering the entire physiological oxygen range and the extremes of hypoxia
- Respiration in the closed chamber or open chamber at steady-state oxygen concentrations
The OROBOROS Oxygraph-2k, unique for high-resolution respirometry (HRR), with the Titration-Injection microPump (TIP2k) on top of the O2k, and the Integrated Suction System (ISS) on the right.
High-resolution respirometry with the OROBOROS Oxygraph-2k established a radically different, integrated systems approach to polarographic measurement of oxygen dynamics, including the DatLab software with on-line display of oxygen concentration and respiration (oxygen flux; see Figure; T-shirt). The mechanics, critical selection of materials (minimum oxygen diffusion), and state-of-the-art electronics provide the basis of signal stability of an optimized polarographic oxygen sensor, in an electronically controlled thermal environment with high temperature stability (0.001 °C). Calibrations and instrumental controls follow standardized HRR protocols, with automatic on-line analysis, including corrections for instrumental background oxygen flux (equation in Figure). Scientific support by the OROBOROS O2k-Team is complementary to the approach of high-resolution respirometry.
High-resolution respirometry is the result of our long-term expertise in instrumental design, software development, mitochondrial physiology, clinical and pharmacological applications, and world-wide scientific cooperation.
