O2k-Fluo

The O2k-Fluo enables the following applications:

oxygen consumption
H₂O₂ production
ATP production
Ca²⁺
mitochondrial membrane potential

The O2k-Fluo also supports the PB-Module, ISE TPP-Module, ISE pH-Module.

This package is available as a Power Package to increase throughput. Certain auxiliary tools and services are not included in Power Packages.
This package is also available without integrated PC. Certain items are not included in O2k-Packages without an integrated PC.

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For O2k Series J and upwards, DatLab 8 ensures proper operation and data acquisition.

Award-winning innovation

Additional information

Oxygen	Respiration chambers with minimum oxygen diffusion • High-resolution of oxygen flux: ±1 pmol O₂ ∙s⁻¹∙mL⁻¹ in normoxia to anoxia • High-resolution of oxygen concentration: 5 nM • Long-term stability and linearity of the polarographic oxygen sensor (POS) • Barometric pressure transducer for accurate air calibration at any altitude
SUIT protocols	Standardized substrate-uncoupler-inhibitor-titration DL-Protocols • Real-time monitoring of respiratory rates and states • Multiple titrations within a single assay for in-depth analysis of mitochondrial fitness
MultiSensor	Additional parameters recorded real-time in combination with oxygen in the same chamber • Automatic and documented calibration routines, instrumental background tests, and mitochondrial assays supported by DL-Protocols
Temperature	4 to 47 °C; stability ±0.002 °C
Thermostat	Peltier temperature control
Chamber	Material: Duran glass Volume: 2 mL or 0.5 mL
Specialized DatLab Software	Installed on integrated computer for protocol design, control and data analysis
Max. power input	330 W
Current at 100-120 V	max. 3.0 A
Current at 220-240 V	max. 1.5 A
Power consumption	During standard operation with screen (when block temperature is stable at 37 °C and ambient temperature of 22 °C) approx. 45-50 W
Lab space required	The Oroboros requires a minimum of lab space (0.5 m bench space), with sufficient space for the ISS, Titration Set and handling. For maximum signal stability and optimal temperature control, the Oroboros should be placed in a position in which it is not exposed to direct sunlight and sudden changes of room temperature. Keep the space on the table below the Oroboros bottom clear to prevent any interference of ventilation beneath the Main Unit.

Configuration

O2k: Hardware and DatLab Software*

Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals
2 Sockets for PB-Module
2 Chambers cV (2.0 mL, Duran® glass)
2 SmartPOS
2 Stirrer Bars cV (white PVDF) , electromagnetic control
2 Stoppers cV (black PEEK) with O-rings cV
2 Chamber Holders cV (blue POM) with O-ring and V-ring
2 POS Holders (blue POM)

Current DatLab version with free follow-up upgrades within the warranty period

O2k: Accessories and Consumables

1 ISS-Integrated Suction System, to remove medium from the Chamber
1 Oxia – the O₂ regime controller for HyperOxia and HypOxia
1 Titration Set: Hamilton microsyringes; fixed needles with rounded tips (8 x 10 mm³ and 3 spare plungers, 6 x 25 mm³, 2 x 50 mm³, 1 x 100 mm³, 1 x 500 mm³), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 Pkg. POS Seal Tip
2 Pkg. POS Membranes
1 POS Electrolyte Powder
1 POS Mounting Tool
1 Polishing Cloth
1 Polishing Powder 0.3 μm
1 O2-Zero Powder
1 Forceps for membrane application
1 Pipette Plastic ungraded
1 Pkg. Conditioning cloths for stainless-steel housing

Fluo Smart-Module: an amperometric add-on module for measurement of hydrogen peroxide production, ATP production, mt-membrane potential, Ca²⁺

2 SmartFluo Sensors, green LED emission, photodiode and filters, excitation LED 525 nm, pre-calibrated intensity
2 SmartFluo Sensors, blue LED emission, photodiode and filters, excitation LED 465 nm, pre-calibrated intensity
3 Filter Sets (Mg green/Ca Green, Amplex® UltraRed, Safranin) consisting of LED filters and photodiode filters

TIP2k-Module: add-on for two-channel operation with the Oroboros with automatic control by DatLab of programmable titration regimes and feedback control

4 Micro-injection syringes, 200 mm³, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip
1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool
1 TIP2k-Needle Safety Support with cable guide
1 Pkg. TIP2k-Filter Papers (10/Pkg.)

sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

2 Chambers sV (Duran® glass), with smaller operation volume (V) of 0.5 mL
2 Stirrer Bars sV (white PVDF) for small volume chambers, electromagnetic control
2 Stoppers sV (black PEEK) for small volume chambers with O-ring sV
2 Chamber Holders sV (black POM) with O-ring and V-ring
2 POS Holders sV (black POM)

Included extras

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 Installation and startup support session (remote, to be used within the warranty period)
1 Oroboros Workshop registration fee for the participation of one lab member waived (to be used within the warranty period)
3-year warranty
* Integrated computer with touch screen (15,6″; FHD 1920×1080) connects DatLab to the NextGen-O2k
* Keyboard set, webcam and webcam accessories

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Additional information

Configuration

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