Startup O2k-Respirometer

Description

The Startup O2k-Respirometer includes the O2k-Main Unit with stainless steel housing, O2k-Assembly Kit, two OroboPOS (polarographic oxygen sensors) and OroboPOS-Service Kit, DatLab software, the ISS-Integrated Suction System, the O2k-Titration Set, and for performing high-resolution respirometry with reduced amounts of biological sample the O2k-sV-Module.

The Startup O2k-Respirometer does not include, but fully supports O2k-Fluorometry and the O2k−Modules: TIP2k, pH, TPP+, NO.

Parameters:

oxygen consumption

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Additional information

Weight	14 kg
Dimensions	45 × 31 × 23 cm
Oxygen	Respiration chambers with minimum oxygen diffusion • High-resolution of oxygen flux: ±1 pmol O2∙s-1∙mL-1 in the from normoxia to anoxia • High-resolution of oxygen concentration: 5nm • Long-term stability and linearity of the polarographic oxygen sensor (OroboPOS) • Barometric pressure transducer for accurate air calibration at any altitude
SUIT protocols	Standardized substrate-uncoupler-inhibitor-titration DL-Protocols • Real-time monitoring of respiratory rates and states • Multiple titrations within a single assay for in-depth analysis of mitochondrial fitness
MultiSensor	Additional parameters recorded real-time in combination with oxygen in the same chamber • Automatic and documented calibration routines, instrumental background tests, and mitochondrial assays supported by DL-Protocols
Temperature	4 to 47 °C; stability ±0.002 °C
Thermostat	Peltier temperature control
Chamber	Material: Duran glass Volume: 2mL or 0.5 mL
Specialized DatLab Software	For use on a Windows PC connected to the O2k via a USB port
Max. power input	120 W
Current at 100-120 V	max. 1.0 A
Current at 220-240 V	max. 0.5 A
Power consumption	During standard operation (when block temperature is stable at 37 °C and ambient temperature of 22 °C) approx. 24 W
Lab space required	The O2k requires a minimum of lab space (0.5 m bench space) next to an external PC or laptop, with sufficient space for the ISS, Titration Set and handling. For maximum signal stability and optimal temperature control, the O2k should be placed in a position in which it is not exposed to direct sunlight and sudden changes of room temperature. Keep the space on the table below the O2k bottom clear to prevent any interference of ventilation beneath the O2k-Main Unit.

Configuration

Oroboros-2k: Hardware and DatLab Software

Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Duran glass chambers
2 OroboPOS polarographic oxygen sensors
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 PVDF stirrers, electromagnetic control
2 PEEK stoppers
Current DatLab version with free follow-up upgrades within the warranty period

Oroboros-O2k: Accessories and Consumables

1 ISS-Integrated Suction System, to remove medium from the O2k-Chamber
1 O2k-Titration Set: Hamilton microsyringes; fixed needles with rounded tips (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 MitoKit-CII: Cell permeable succinate (25 mg) and malonate (10 mg)

O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

2 Duran glass chambers, with smaller operation volume (V) of 0.5 cm3 (0.5 mL)
2 PVDF stirrers for small volume chambers, electromagnetic control
2 PEEK stoppers for small volume chambers
2 Black POM chamber holders with O-ring and V-ring
2 Black POM OroboPOS-holders

Included extras

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 O2k-Workshop registration for one lab member within the warranty period

Bioblast resources

Manuals:

O2k-Manual

MitoPedia methods:

Respirometry

Guides:

O2k-Demo experiments

O2k hardware