Power O2k-Respirometer

An economical option for using additional O2k-Units to increase output in high-resolution respirometry.

Product ID: 10023-03

Description

Multiple O2k-units are offered as a Power O2k-Respirometer system for high-resolution combined with high-output. Many O2k-Network Labs have increased their high-resolution high-output by multiple O2k-units, using two to 12 O2k (4 to 24 O2k-Chambers).

This application does not require an additional ISS-Integrated Suction System, an O2k-Titration Set and the O2k-Manual. Furthermore, the OroboPOS-Mounting Tool of the OroboPOS Service Tools can be used from the available O2k and is not included.

The Power O2k-Respirometer supports all add-on O2k-Modules of the Oroboros O2k.

Parameters:

  • oxygen consumption

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Additional information

Oxygen

Respiration chambers with minimum oxygen diffusion
• High-resolution of oxygen flux: ±1 pmol O2∙s-1∙mL-1 in the from normoxia to anoxia
• High-resolution of oxygen concentration: 5nm
• Long-term stability and linearity of the polarographic oxygen sensor (OroboPOS)
• Barometric pressure transducer for accurate air calibration at any altitude

SUIT protocols

Standardized substrate-uncoupler-inhibitor-titration DL-Protocols
• Real-time monitoring of respiratory rates and states
• Multiple titrations within a single assay for in-depth analysis of mitochondrial fitness

MultiSensor

Additional parameters recorded real-time in combination with oxygen in the same chamber
• Automatic and documented calibration routines, instrumental background tests, and mitochondrial assays supported by DL-Protocols

Temperature

4 to 47 °C; stability ±0.002 °C

Thermostat

Peltier temperature control

Chamber

Material: Duran glass
Volume: 2mL or 0.5 mL

Specialized DatLab Software

For use on a Windows PC connected to the O2k via a USB port

Max. power input

120 W

Current at 100-120 V

max. 1.0 A

Current at 220-240 V

max. 0.5 A

Power consumption

During standard operation (when block temperature is stable at 37 °C and ambient temperature of 22 °C) approx. 24 W

Lab space required

The O2k requires a minimum of lab space (0.5 m bench space) next to an external PC or laptop, with sufficient space for the ISS, Titration Set and handling.
For maximum signal stability and optimal temperature control, the O2k should be placed in a position in which it is not exposed to direct sunlight and sudden changes of room temperature. Keep the space on the table below the O2k bottom clear to prevent any interference of ventilation beneath the O2k-Main Unit.

Configuration

Oroboros-O2k: Hardware and DatLab Software

Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Duran glass chambers
2 OroboPOS polarographic oxygen sensors
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 PVDF stirrers, electromagnetic control
2 PEEK stoppers
Current DatLab version with free follow-up upgrades within the warranty period

Oroboros-O2k: Accessories and Consumables

1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 MitoKit-CII: Cell permeable succinate (25 mg) and malonate (10 mg)

O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

2 Duran glass chambers, with smaller operation volume (V) of 0.5 cm3 (0.5 mL)
2 PVDF stirrers for small volume chambers, electromagnetic control
2 PEEK stoppers for small volume chambers
2 Black POM chamber holders with O-ring and V-ring
2 Black POM OroboPOS-holders

Included extras

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 O2k-Workshop registration for one lab member within the warranty period

Bioblast resources

This link will take you to the Bioblast page for this product where you will find manuals, guides and other relevant information.

Power O2k-Respirometer