Power O2k-FluoRespirometer

Description

The Power O2k-FluoRespirometer includes the TIP2k and the O2k-sV-Module, and supports all add-on O2k-Modules of the Oroboros O2k. It can be added to an existing Oroboros O2k of any O2k-Series. This application does not require an additional ISS-Integrated Suction System and O2k-Titration Set. Furthermore, the OroboPOS-Mounting Tool of the OroboPOS Service Tools can be used from the available O2k and is not included.

Parameters:

oxygen consumption
ROS production
ATP production
Ca²⁺
mitochondrial membrane potential

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Additional information

Oxygen	Respiration chambers with minimum oxygen diffusion • High-resolution of oxygen flux: ±1 pmol O2∙s-1∙mL-1 in the from normoxia to anoxia • High-resolution of oxygen concentration: 5nm • Long-term stability and linearity of the polarographic oxygen sensor (OroboPOS) • Barometric pressure transducer for accurate air calibration at any altitude
SUIT protocols	Standardized substrate-uncoupler-inhibitor-titration DL-Protocols • Real-time monitoring of respiratory rates and states • Multiple titrations within a single assay for in-depth analysis of mitochondrial fitness
MultiSensor	Additional parameters recorded real-time in combination with oxygen in the same chamber • Automatic and documented calibration routines, instrumental background tests, and mitochondrial assays supported by DL-Protocols
Temperature	4 to 47 °C; stability ±0.002 °C
Thermostat	Peltier temperature control
Chamber	Material: Duran glass Volume: 2mL or 0.5 mL
Specialized DatLab Software	For use on a Windows PC connected to the O2k via a USB port
Max. power input	120 W
Current at 100-120 V	max. 1.0 A
Current at 220-240 V	max. 0.5 A
Power consumption	During standard operation (when block temperature is stable at 37 °C and ambient temperature of 22 °C) approx. 24 W
Lab space required	The O2k requires a minimum of lab space (0.5 m bench space) next to an external PC or laptop, with sufficient space for the ISS, Titration Set and handling. For maximum signal stability and optimal temperature control, the O2k should be placed in a position in which it is not exposed to direct sunlight and sudden changes of room temperature. Keep the space on the table below the O2k bottom clear to prevent any interference of ventilation beneath the O2k-Main Unit.

Configuration

O2k-FluoRespirometer: Hardware and DatLab Software

Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Duran glass chambers
2 OroboPOS polarographic oxygen sensors
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 PVDF stirrers, electromagnetic control
2 PEEK stoppers
2 Smart Fluo-Sensors, green LED emission, photodiode and filters, excitation LED 525 nm, pre-calibrated intensity
2 Smart Fluo-Sensors, blue LED emission, photodiode and filters, excitation LED 465 nm, pre-calibrated intensity
3 Filter Sets (Mg green/Ca Green, Amplex Red, Safranin) consisting of LED filters and photodiode filters
Current DatLab version with free follow-up upgrades within the warranty period

O2k-FluoRespirometer: Accessories and Consumables

1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 MitoKit-CII: Cell permeable succinate (25 mg) and malonate (10 mg)

TIP2k-Module: add-on for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control

4 Microinjection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip
1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool
1 TIP2k-Needle Safety Support with cable guide
1 Pkg. TIP2k-Filter Papers (10/Pkg.)

O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

2 Duran glass chambers, with smaller operation volume (V) of 0.5 cm3 (0.5 mL)
2 PVDF stirrers for small volume chambers, electromagnetic control
2 PEEK stoppers for small volume chambers
2 Black POM chamber holders with O-ring and V-ring
2 Black POM OroboPOS-holders

Included extras

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 O2k-Workshop registration for one lab member within the warranty period

Bioblast resources

Manuals:

O2k-FluoRespirometer

MitoPedia methods:

Guides: