The Power O2k-FluoRespirometer includes the TIP2k and the O2k-sV-Module, and supports all add-on O2k-Modules of the Oroboros O2k. It can be added to an existing Oroboros O2k of any O2k-Series. This application does not require an additional ISS-Integrated Suction System and O2k-Titration Set. Furthermore, the OroboPOS-Mounting Tool of the OroboPOS Service Tools can be used from the available O2k and is not included.
Parameters:
- oxygen consumption
- ROS production
- ATP production
- Ca2+
- mitochondrial membrane potential
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Configuration
O2k-FluoRespirometer: Hardware and DatLab Software
Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 OroboPOS polarographic oxygen sensors
2 Duran® glass chambers
2 PVDF stirrers, electromagnetic control
2 PEEK stoppers with O-ring
2 Blue POM chamber holders with O-ring and V-ring
2 Black POM OroboPOS-holders
2 Smart Fluo-Sensors, green LED emission, photodiode and filters, excitation LED 525 nm, pre-calibrated intensity
2 Smart Fluo-Sensors, blue LED emission, photodiode and filters, excitation LED 465 nm, pre-calibrated intensity
3 Filter Sets (Mg green/Ca Green, Amplex® UltraRed, Safranin) consisting of LED filters and photodiode filters
Current DatLab version with free follow-up upgrades within the warranty period
O2k-FluoRespirometer: Accessories and Consumables
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 Pkg. OroboPOS-Seal Tip
1 Pkg. OroboPOS-Membranes
1 OroboPOS-Electrolyte Powder
1 OroboPOS-Mounting Tool
1 OroboPOS-Polishing Cloth
1 OroboPOS-Polishing Powder
1 O2-Zero Powder
1 Forceps for membrane application
1 Pipette\Plastic\ungraded
TIP2k-Module: add-on for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control
4 Micro-injection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip
1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool
1 TIP2k-Needle Safety Support with cable guide
1 Pkg. TIP2k-Filter Papers (10/Pkg.)
O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function
2 Duran® glass chambers, with smaller operation volume (V) of 0.5 mL
2 PVDF stirrers for small volume chambers, electromagnetic control
2 PEEK stoppers for small volume chambers with O-ring
2 Black POM chamber holders with O-ring and V-ring
2 Black POM OroboPOS-holders
Included extras
Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 O2k-Workshop registration for one lab member within the warranty period