O2k-FluoRespirometer

Our flagship package is the complete experimental system for high-resolution respirometry (HRR), including fluorometry, the TIP2k and the O2k-sV-Module allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+ (CaG) or ATP production (MgG).

Product ID: 10002-03


 

Description

The O2k-FluoRespirometer supports all add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrodes (ISE for TPP+ or TPMP+ ) or pH.

Parameters:

  • oxygen consumption
  • ROS production
  • ATP production
  • Ca2+
  • mitochondrial membrane potential

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Configuration

O2k-FluoRespirometer: Hardware and DatLab Software

Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Duran® glass chambers
3 OroboPOS polarographic oxygen sensors (with 1 spare POS)
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 PVDF stirrers, electromagnetic control
2 PEEK stoppers
2 Smart Fluo-Sensors, green LED emission, photodiode and filters, excitation LED 525 nm, pre-calibrated intensity
2 Smart Fluo-Sensors, blue LED emission, photodiode and filters, excitation LED 465 nm, pre-calibrated intensity
3 Filter Sets (Mg green/Ca Green, Amplex® UltraRed, Safranin) consisting of LED filters and photodiode filters
Current DatLab version with free follow-up upgrades within the warranty period

O2k-FluoRespirometer: Accessories and Consumables                                                                                                  

1 ISS-Integrated Suction System, to remove medium from the O2k-Chamber
1 O2k-Titration Set: Hamilton microsyringes; fixed needles with rounded tips (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 MitoKit-CII: Cell permeable succinate (25 mg) and malonate (10 mg)

TIP2k-Module: add-on for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control

4 Microinjection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip
1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool
1 TIP2k-Needle Safety Support with cable guide
1 Pkg. TIP2k-Filter Papers (10/Pkg.)

O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

2 Duran® glass chambers, with smaller operation volume (V) of 0.5 mL
2 PVDF stirrers for small volume chambers, electromagnetic control
2 PEEK stoppers for small volume chambers
2 Black POM chamber holders with O-ring and V-ring
2 Black POM OroboPOS-holders

Included extras                                                                                           

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 O2k-Workshop registration for one lab member within the warranty period

 

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Bioblast resources

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O2k-FluoRespirometer