O2k-FluoRespirometer

Our flagship package is the complete experimental system for high-resolution respirometry (HRR), including fluorometry, the TIP2k and the O2k-sV-Module allowing simultaneous monitoring of oxygen consumption together with either ROS production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+ (CaG) or ATP production (MgG).

Product ID: 10002-03


 

Description

The O2k-FluoRespirometer supports all add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrodes (ISE for TPP+ or TPMP+ ) or pH.

Parameters:

  • oxygen consumption
  • ROS production
  • ATP production
  • Ca2+
  • mitochondrial membrane potential

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Additional information

Oxygen

Respiration chambers with minimum oxygen diffusion
• High-resolution of oxygen flux: ±1 pmol O₂ ∙s⁻¹∙mL⁻¹ in normoxia to anoxia
• High-resolution of oxygen concentration: 5 nM
• Long-term stability and linearity of the polarographic oxygen sensor (OroboPOS)
• Barometric pressure transducer for accurate air calibration at any altitude

SUIT protocols

Standardized substrate-uncoupler-inhibitor-titration DL-Protocols
• Real-time monitoring of respiratory rates and states
• Multiple titrations within a single assay for in-depth analysis of mitochondrial fitness

MultiSensor

Additional parameters recorded real-time in combination with oxygen in the same chamber
• Automatic and documented calibration routines, instrumental background tests, and mitochondrial assays supported by DL-Protocols

Temperature

4 to 47 °C; stability ±0.002 °C

Thermostat

Peltier temperature control

Chamber

Material: Duran glass
Volume: 2 mL or 0.5 mL

Specialized DatLab Software

For use on a Windows PC connected to the O2k via a USB port

Max. power input

120 W

Current at 100-120 V

max. 1.0 A

Current at 220-240 V

max. 0.5 A

Power consumption

During standard operation (when block temperature is stable at 37 °C and ambient temperature of 22 °C) approx. 24 W

Lab space required

The O2k requires a minimum of lab space (0.5 m bench space) next to an external PC or laptop, with sufficient space for the ISS, Titration Set and handling.
For maximum signal stability and optimal temperature control, the O2k should be placed in a position in which it is not exposed to direct sunlight and sudden changes of room temperature. Keep the space on the table below the O2k bottom clear to prevent any interference of ventilation beneath the O2k-Main Unit.

Configuration

O2k-FluoRespirometer: Hardware and DatLab Software

Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Duran glass chambers
3 OroboPOS polarographic oxygen sensors (with 1 spare POS)
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 PVDF stirrers, electromagnetic control
2 PEEK stoppers
2 Smart Fluo-Sensors, green LED emission, photodiode and filters, excitation LED 525 nm, pre-calibrated intensity
2 Smart Fluo-Sensors, blue LED emission, photodiode and filters, excitation LED 465 nm, pre-calibrated intensity
3 Filter Sets (Mg green/Ca Green, Amplex Red, Safranin) consisting of LED filters and photodiode filters
Current DatLab version with free follow-up upgrades within the warranty period

O2k-FluoRespirometer: Accessories and Consumables                                                                                                  

1 ISS-Integrated Suction System, to remove medium from the O2k-Chamber
1 O2k-Titration Set: Hamilton microsyringes; fixed needles with rounded tips (6 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 1 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 MitoKit-CII: Cell permeable succinate (25 mg) and malonate (10 mg)

TIP2k-Module: add-on for two-channel operation with the O2k-FluoRespirometer with automatic control by DatLab of programmable titration regimes and feedback control

4 Microinjection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip
1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool
1 TIP2k-Needle Safety Support with cable guide
1 Pkg. TIP2k-Filter Papers (10/Pkg.)

O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

2 Duran glass chambers, with smaller operation volume (V) of 0.5 cm3 (0.5 mL)
2 PVDF stirrers for small volume chambers, electromagnetic control
2 PEEK stoppers for small volume chambers
2 Black POM chamber holders with O-ring and V-ring
2 Black POM OroboPOS-holders

Included extras                                                                                           

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 O2k-Workshop registration for one lab member within the warranty period

Bioblast resources

This link will take you to the Bioblast page for this product where you will find manuals, guides and other relevant information.

O2k-FluoRespirometer