NextGen-O2k all-in-one

The complete experimental system for high-resolution fluorespirometry, redox biology and PhotoBiology: allowing simultaneous monitoring of oxygen consumption together with either coenzyme Q redox state, NAD(P)H redox state, cyclic voltammetry, H2O2 production (AmR), mt-membrane potential (TMRM, Safranin and Rhodamine 123), Ca2+uptake capacity (CaG), ATP production (MgG) or photobiology measurements.

Product ID: 10101-01

The NextGen-O2k all-in-one enables the following O2k-Applications:

  • oxygen consumption
  • Q-redox state
  • NAD(P)H-redox state
  • cyclic voltammetry
  • H2O2 production
  • ATP production
  • Ca2+
  • mitochondrial membrane potential
  • photosynthesis
  • light-enhanced dark respiration

The NextGen-O2k all-in-one also supports all add-on O2k-Modules: O2k-TPP+ ISE-Module, O2k-pH ISE-Module, O2k-NO Amp-Module, enabling measurement of mt-membrane potential with ion sensitive electrodes (ISE for TPP+ or TPMP+ ) or pH.

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Additional information

Oxygen

Respiration chambers with minimum oxygen diffusion
• High-resolution of oxygen flux: ±1 pmol O₂ ∙s⁻¹∙mL⁻¹ in normoxia to anoxia
• High-resolution of oxygen concentration: 5 nM
• Long-term stability and linearity of the polarographic oxygen sensor (OroboPOS)
• Barometric pressure transducer for accurate air calibration at any altitude

SUIT protocols

Standardized substrate-uncoupler-inhibitor-titration DL-Protocols
• Real-time monitoring of respiratory rates and states
• Multiple titrations within a single assay for in-depth analysis of mitochondrial fitness

MultiSensor

Additional parameters recorded real-time in combination with oxygen in the same chamber
• Automatic and documented calibration routines, instrumental background tests, and mitochondrial assays supported by DL-Protocols

Temperature

4 to 47 °C; stability ±0.002 °C

Thermostat

Peltier temperature control

Chamber

Material: Duran glass
Volume: 2 mL or 0.5 mL

Specialized DatLab Software

Installed on integrated computer for protocol design, control and data analysis

Max. power input

330 W

Current at 100-120 V

max. 3.0 A

Current at 220-240 V

max. 1.5 A

Power consumption

During standard operation with screen (when block temperature is stable at 37 °C and ambient temperature of 22 °C) approx. 45-50 W

Lab space required

The O2k requires a minimum of lab space (0.5 m bench space), with sufficient space for the ISS, Titration Set and handling.
For maximum signal stability and optimal temperature control, the O2k should be placed in a position in which it is not exposed to direct sunlight and sudden changes of room temperature. Keep the space on the table below the O2k bottom clear to prevent any interference of ventilation beneath the O2k-Main Unit.

Configuration

NextGen-O2k: Hardware and DatLab Software*

Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 Sockets for Q-and NAD(P)H-Modules
2 Sockets for PB-Module
3 OroboPOS polarographic oxygen sensors (with 1 spare POS)
2 Duran® glass chambers
2 PVDF stirrers, electromagnetic control
2 PEEK stoppers with O-ring
2 Blue POM chamber holders with O-ring and V-ring
2 Blue POM OroboPOS-holders

Current DatLab version with free follow-up upgrades within the warranty period

O2k-Fluo Smart-Module: an amperometric add-on module for measurement of hydrogen peroxide production, ATP production, mt-membrane potential, Ca2+

2 Smart Fluo-Sensors, green LED emission, photodiode and filters, excitation LED 525 nm, pre-calibrated intensity
2 Smart Fluo-Sensors, blue LED emission, photodiode and filters, excitation LED 465 nm, pre-calibrated intensity
3 Filter Sets (Mg green/Ca Green, Amplex® UltraRed, Safranin) consisting of LED filters and photodiode filters

Q-Module: for measuring the Q-redox state and cyclic voltammetry

2 Reference electrodes, 2.4mm Ø glass shaft
10 Replacement barrels for reference electrodes , 2.4 mm glass
Q-Stoppers\beige PEEK\conical shaft\side+2.6 mm+1.3 mm central port: with glassy carbon and platinum electrode embedded
1 Electrolyte\Reference-Electrode
1 ISE-Filling Syringe with 2 needles
2 Polishing Powder 0.3 μm
2 Polishing Powder 0.05 µm/a>
4
Polishing Cloth
3 Pkgs Q-Filter Papers (10/Pkg)
2 Boxes O-ring\Viton®\12.5×1 mm, 8/box

NADH-Module: for simultaneous measurement of oxygen consumption and NAD(P)H autofluorescence.

2 NADH-Sensors
2 PEEK stirrers
3 Pkg of Filter sets R370 for NADH (12/Pkg)

PB-Module: for conducting measurements of PhotoBiology, including photosynthesis
6 PhotoBiology light sources – PB-Light Sources (2 blue, 2 red and 2 white)

NextGen-O2k: Accessories and Consumables                                                                                                  

1 ISS-Integrated Suction System, to remove medium from the O2k-Chamber
1 Oxia – the O2 regime controller for HyperOxia and HypOxia
1 O2k-Titration Set: Hamilton microsyringes; fixed needles with rounded tips (8 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 2 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 Pkg. OroboPOS-Seal Tip
1 Pkg. OroboPOS-Membranes
1 OroboPOS-Electrolyte Powder
1 OroboPOS-Mounting Tool
1 Polishing Cloth
1 Polishing Powder 0.3 μm
1 O2-Zero Powder
1 Forceps for membrane application
1 Pipette\Plastic\ungraded
1 Pkg. Conditioning cloths for stainless-steel housing

TIP2k-Module: add-on for two-channel operation with the O2k with automatic control by DatLab of programmable titration regimes and feedback control

4 Micro-injection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip
1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool
1 TIP2k-Needle Safety Support with cable guide
1 Pkg. TIP2k-Filter Papers (10/Pkg.)

O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

2 Duran® glass chambers, with smaller operation volume (V) of 0.5 mL
2 PVDF stirrers for small volume chambers, electromagnetic control
2 PEEK stoppers for small volume chambers with O-ring
2 Black POM chamber holders with O-ring and V-ring
2 Black POM OroboPOS-holders

Included extras                                                                                           

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 Installation and startup support session (to be used within the warranty period)
3-year warranty
* Integrated computer with touch screen (15,6″; FHD 1920×1080) connects DatLab to the NextGen-O2k
* Keyboard, webcam and webcam accessories

 

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Bioblast resources

This link will take you to the Bioblast page for this product where you will find manuals, guides and other relevant information.

NextGen-O2k manual

NextGen-O2k: Q-Module manual

NextGen-O2k: NADH-Module manual