NextGen O2k-FluoRespirometry and Redox

Configuration

NextGen-O2k: Hardware and DatLab Software*

Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 Sockets for Q-and NAD(P)H-Modules
2 Sockets for PB-Module
3 OroboPOS polarographic oxygen sensors (with 1 spare POS)
2 Duran® glass chambers
2 PVDF stirrers, electromagnetic control
2 PEEK stoppers with O-ring
2 Blue POM chamber holders with O-ring and V-ring
2 Blue POM OroboPOS-holders

Current DatLab version with free follow-up upgrades within the warranty period

O2k-Fluo Smart-Module: an amperometric add-on module for measurement of hydrogen peroxide production, ATP production, mt-membrane potential, Ca²⁺

2 Smart Fluo-Sensors, green LED emission, photodiode and filters, excitation LED 525 nm, pre-calibrated intensity
2 Smart Fluo-Sensors, blue LED emission, photodiode and filters, excitation LED 465 nm, pre-calibrated intensity
3 Filter Sets (Mg green/Ca Green, Amplex® UltraRed, Safranin) consisting of LED filters and photodiode filters

Q-Module: for measuring the Q-redox state and cyclic voltammetry

2 Reference electrodes, 2.4mm Ø glass shaft

10 Replacement barrels for reference electrodes , 2.4 mm glass
4 Q-Stopper beige PEEK conical shaft side+2.6 mm+1.3 mm central port: with glassy carbon and platinum electrode embedded
1 ElectrolyteReference-Electrode
1 ISE-Filling Syringe with 2 needles
2 Polishing Powder 0.3 μm
2 Polishing Powder 0.05 μm
4 Polishing Cloth
3 Q-Filter Papers; 10/Pkg
2 Boxes O-ringViton®12.5×1 mm, 8/box

NADH-Module: for simultaneous measurement of oxygen consumption and NAD(P)H autofluorescence.

2 NADH-Sensors
3 Filter-set R370 for NADH (12/Pkg)

NextGen-O2k: Accessories and Consumables                                                                                                  

1 ISS-Integrated Suction System, to remove medium from the O2k-Chamber
1 O2k-Titration Set: Hamilton microsyringes; fixed needles with rounded tips (8 x 10 mm3 and 3 spare plungers, 6 x 25 mm3, 2 x 50 mm3, 1 x 100 mm3, 1 x 500 mm3), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
1 Pkg. OroboPOS-Seal Tip
1 Pkg. OroboPOS-Membranes
1 OroboPOS-Electrolyte Powder
1 OroboPOS-Mounting Tool
1 Polishing Cloth
1 Polishing Powder 0.3 μm
1 O2-Zero Powder
1 Forceps for membrane application
1 PipettePlasticungraded
1 Pkg. Conditioning cloths for stainless-steel housing

TIP2k-Module: add-on for two-channel operation with the O2k with automatic control by DatLab of programmable titration regimes and feedback control

4 Micro-injection syringes, 200 mm3, 180 mm needle length, 0.12 mm inner Ø; fixed needles with rounded tip
1 Pkg. Needle Spacers, silicone stops (200/Pkg.) with mounting tool
1 TIP2k-Needle Safety Support with cable guide
1 Pkg. TIP2k-Filter Papers (10/Pkg.)

O2k-sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

2 Duran® glass chambers, with smaller operation volume (V) of 0.5 mL
2 PVDF stirrers for small volume chambers, electromagnetic control
2 PEEK stoppers for small volume chambers with O-ring
2 Black POM chamber holders with O-ring and V-ring
2 Black POM OroboPOS-holders

Included extras                                                                                           

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 Installation and startup support session (to be used within the warranty period)

* Integrated computer with touch screen (15,6″; FHD 1920×1080) connects DatLab to the NextGen-O2k

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