MitoAnalytics Power O2k

Focusing on decreased sample amount and increased throughput, the MitoAnalytics Power O2k features two O2k-Main Units, Series J with integrated PC with small volume chambers (0.5 mL).

It enables the following applications:

oxygen consumption in SUIT protocols

The MitoAnalytics Power O2k does not include classic volume chambers (2.0 mL). Separate purchase required to support Oroboros Fluorometry and the add-on Modules: PB-Module, TPP ISE-Module, pH ISE-Module. The TIP2k-Module is not included but fully supported.

This package is also available without integrated PC. Certain items are not included in O2k-Packages without an integrated PC.

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For O2k Series J and upwards, DatLab 8 ensures proper operation and data acquisition for all Oroboros Modules.

Award-winning innovation

Additional information

Oxygen	Respiration chambers with minimum oxygen diffusion • High-resolution of oxygen flux: ±1 pmol O₂ ∙s⁻¹∙mL⁻¹ in normoxia to anoxia • High-resolution of oxygen concentration: 5 nM • Long-term stability and linearity of the polarographic oxygen sensor (POS) • Barometric pressure transducer for accurate air calibration at any altitude
SUIT protocols	Standardized substrate-uncoupler-inhibitor-titration DL-Protocols • Real-time monitoring of respiratory rates and states • Multiple titrations within a single assay for in-depth analysis of mitochondrial fitness
Temperature	4 to 47 °C; stability ±0.002 °C
Thermostat	Peltier temperature control
Chamber	Material: Duran glass Volume: 2 mL or 0.5 mL
Specialized DatLab Software	Installed on integrated computer for protocol design, control and data analysis
Max. power input	330 W
Current at 100-120 V	max. 3.0 A
Current at 220-240 V	max. 1.5 A
Power consumption	During standard operation with screen (when block temperature is stable at 37 °C and ambient temperature of 22 °C) approx. 45-50 W
Lab space required	Each Oroboros requires a minimum of lab space (0.5 m bench space), with sufficient space for the ISS, Titration Set and handling. For maximum signal stability and optimal temperature control, the Oroboros should be placed in a position in which it is not exposed to direct sunlight and sudden changes of room temperature. Keep the space on the table below the Oroboros bottom clear to prevent any interference of ventilation beneath the Main Unit.

Configuration

O2k: Hardware and DatLab Software*

2 Oroboros O2k-Main Unit, Series J, with integrated PC, each equipped with
Stainless steel housing
Peltier thermostat 4 to 47 °C; ±0.002 °C
Barometric pressure transducer
2 Amperometric oxygen channels
2 ISE sockets for ion sensitive electrodes
2 Sockets for optical signals or NO
2 Sockets for Q-and NAD(P)H-Modules
2 Sockets for PB-Module

2 SmartPOS
2 POS Holders (blue POM)

Current DatLab version with free follow-up upgrades within the warranty period

O2k: Accessories and Consumables

1 ISS-Integrated Suction System, to remove medium from the Chamber
1 Titration Set: Hamilton microsyringes; fixed needles with rounded tips (8 x 10 mm³ and 3 spare plungers, 6 x 25 mm³, 2 x 50 mm³, 1 x 100 mm³, 1 x 500 mm³), packed in syringe storage box with syringe labels, 2 x syringe racks with syringe collars, 2 x tube racks
1 MiR05-Kit: Mitochondrial Respiration Medium. For a final volume of 250 mL
2 Pkg. POS Seal Tip
4 Pkg. POS Membranes
2 POS Electrolyte Powder
1 POS Mounting Tool
2 Polishing Cloth
2 Polishing Powder 0.3 μm
2 O2-Zero Powder
2 Forceps for membrane application
2 Pipette Plastic ungraded
2 Pkg. Conditioning cloths for stainless-steel housing

2 sV-Module: small chamber volume to reduce the sample amount needed for OXPHOS analysis and cellular/mitochondrial respiratory function

4 Chambers sV (Duran® glass), with smaller operation volume (V) of 0.5 mL
4 Stirrer Bars sV (white PVDF) for small volume chambers, electromagnetic control
4 Stoppers sV (black PEEK) for small volume chambers with O-ring sV
4 Chamber Holders sV (black POM) with O-ring and V-ring
4 POS Holders sV (black POM)

Included extras

Packing, handling, worldwide shipping
Remote support from Oroboros experts
1 Installation and startup support session (remote, to be used within the warranty period)
1 Oroboros Workshop registration fee for the participation of one lab member waived (to be used within the warranty period)
3-year warranty
* Integrated computer with touch screen (15,6″; FHD 1920×1080) connects DatLab to the NextGen-O2k
* Keyboard set, webcam and webcam accessories

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Additional information

Configuration

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