O2k-Concept

The Oroboros O2k is the modular system for high-resolution respirometry (HRR) for mitochondria and cell research. HRR is the basis for combined measurement of respiration and ROS production, mitochondrial membrane potential, ATP production, Ca2+, or pH.

With the Oroboros O2k, small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells to living cells and tissues slices.

Experimental data are displayed not only as oxygen concentration over time but also as real-time transformation of these data, allowing the experimenter to follow respiration (oxygen consumption, oxygen flux) of biological and biochemical samples while the experiment is still running.

The modular O2k-concept is supported by DatLab 7 with high flexibility for extension by add-on O2k-Modules or economical first steps with O2k-Packages.

The high-resolution O2k technology is internationally tested and trusted with emphasis on quality and scientific output of research in mitochondrial physiology and pathology.

The Oroboros O2k is the modular system for high-resolution respirometry (HRR) for mitochondria and cell research. HRR is the basis for combined measurement of respiration and ROS production, mitochondrial membrane potential, ATP production, Ca2+, or pH.

With the Oroboros O2k, small amounts of biological samples can be used for bioenergetic and OXPHOS analysis, ranging from isolated mitochondria, permeabilized tissues and permeabilized cells to intact cells and tissues slices.

Experimental data are displayed not only as oxygen concentration over time but also as real-time transformation of these data, allowing the experimenter to follow respiration (oxygen consumption, oxygen flux) of biological and biochemical samples while the experiment is still running.

The modular O2k-concept is supported by DatLab 7 with high flexibility for extension by add-on O2k-Modules or economical first steps with O2k-Packages.

The high-resolution O2k technology is internationally tested and trusted with emphasis on quality and scientific output of research in mitochondrial physiology and pathology.

High output - low running costs

The annual running costs for the O2k are very low, as experienced worldwide by more than 1000 O2k-users. Our high-quality and durable instruments along with reusable chambers mean big savings for our customers.

  • O2k-hardware lifespans of up to over 10 years and less than 10 % of all instruments sold worldwide requiring repair at our workshop
  • Annual costs of less than € 1,000 for O2k-spares (e.g. spare glass chamber, stoppers)
  • Cost effective O2k-consumables, such as the MiR05-Kit costing a maximum of € 1.40 per run
  • Run experiments with low amounts of samples – further conserve with the smaller volume chambers (0.5 mL) of  the O2k-sV-Module
  • No special cleaning detergents needed. Wash parts with deionized or distilled water and ethanol