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• Oxygen signal not responding

Low oxygen signal of the polarographic oxygen sensor (O2S) even at air saturation.

 

• Oxygen signal not stable

High zero signal due to polarographic oxygen sensor (POS) or electronics.

 

• Oxygen signal an POS connector

The signal changes when the O2S connector is touched or moved.

 

• Oxygen calibration changes suddenly

Check O2k setup settings in DatLab, POS membrane, or chamber assembly.

 

• DatLab error messages - problems and solutions.

• Flux indicates an apparently low RCR

In experiments with isolated mitochondria, state 2 - state 3 - state 4 transitions are used for calculating the P/O ratio and respiratory control ratio (RCR). Even with a good mitochondrial preparation, the RCR may appear to be low when observing on-line the peak oxygen flux at state 3. - There is a simple solution.

 

• Oxygen flux lags behind or reaches a low peak value.

Why does the plot of oxygen flux apparently lag behind the time course of oxygen concentration?

Why does oxygen flux reach a low peak value (and a correspondingly low RCR is obtained) when an ADP pulse is added to isolated mitochondria?

 

• Lasting effect of EtOH-soluble inhibitors

Oxygen flux per mg tissue was significantly lower (<50 %) in one particular chamber, compared to parallel experiments in the other chamber(s). The effect was due to incomplete washout of inhibitors used in the titration protocol.

 

• OROBOROS Oxygraph PAAR

Failure of Temperature Control

Recorder Interface

See also

• Notes on POS Service