For a current quotation, please contact us at instruments@oroboros.at.

OROBOROS INSTRUMENTS GmbH

high-resolution respirometry

Schöpfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Email: instruments@oroboros.at

- development - production – distribution -

- application - cooperation – feedback -

 

• Direct WorldWide distribution - from development to your application - represents the successful strategy of OROBOROS INSTRUMENTS, feeding scientific expertise into customer service and saving local distributor costs for direct feedback into science.

• Customer support from our office and research laboratory in Innsbruck is complemented by local scientific support through the WorldWide Mitochondrial Physiology Network. With your cooperation, the world-wide OROBOROS MiPNet will become stronger. Within the MiPNet, the expertise of lead users and customer innovation contribute to the unique standard of high-resolution respirometry.
• Low running costs - lower than in other (semiquantitative) methodological approaches.
• 2 years warranty on all products of OROBOROS INSTRUMENTS (except glass ware).
• PC requirements
• Spare parts and the OROBOROS T-shirt.

•  Advances in HRR. MiPNet07.01 [download pdf 215 kB; 2010-08-17.

On the basis of long-term developments and experimental tests, the OROBOROS team is excited to report about significant improvements of high-resolution respirometry with the Oxygraph-2k: Advances in electronics, smoother stirring at higher rates, higher precision of the glass chambers, and higher temperature stability of the Peltier system made possible a significant improvement of signal stability, beyond the high performance reported previously (Gnaiger 2001 Resp. Physiol. 128: 277). This opens up new perspectives for measurements with small tissue biopsies, culured cells and studies in the low-oxygen range. The electronics can be upgraded for MultiSensor applications of the O2k-MultiSensor System.

The OROBOROS Oxygraph-2k with DatLab 4 software is the sole-source instrument for high-resolution respirometry, with the option of modular MultiSensor extension and electronically controlled Titration-Injection microPump (TIP2k), and accessories including the Integrated Suction System (ISS) and titration syringes.

The OROBOROS O2k-MultiSensor extension is the basis for application of our new Ion Selective Electrode (ISE) system for measurement of pH, mitochondrial membrane potential (TPP+ electrode), and additional electrochemical sensors (NO). Our innovations are at the interface between instrumental development, research in bioenergetics and biomedicine, and world-wide distribution. Whereas the high-resolution analysis of oxygen concentration and flux remains the fundamental basis, the O2k-MultiSensor system integrates specific requirements in mitochondrial physiology with ion-selective electrodes and optical measurements.

Our instruments are represented in leading laboratories for basic and clinical research - OROBOROS WorldWide. The OROBOROS Oxygraph-2k has become the standard where high resolution counts, at low respiratory activities, in particular for the analysis of cell cultures, muscle biopsies, including human applications, and for measurements at critical low oxygen levels. Modular extension is possible, upgrading the Oxygraph-2k to the O2k-MultiSensor System. Combination with the Titration-Injection microPump (TIP2k) allows operation with programmable titration regimes, feedback control, and at steady-states, yielding a new flexibility in experimental design by combining the technical advantages of closed and open systems approaches. These developments lead not merely a system of high quality instruments but distinguish high-resolution respirometry as a method and concept.

2000 OROBOROS^® Oxygraph-2k: 2-chamber titration-injection respirometer:

·       2 glass chambers with PVDF stoppers (improvement with respect to our previous titanium stoppers), PEEK stirrer bars.

·       Electronic Peltier thermostat and 2 magnetic stirrers.

OROBOROS^® DatLab (Windows^™) for MultiChannel data acquisition, control & on-line analysis.

·       Barometric pressure transducer, output to DatLab, graphical and digital display (kPa or mmHg).

·       2 identical polarographic oxygen sensors (POS for chamber A and B) with service kit.

·       System for modular extension to the O2k-MultiSensor System (No. 30100).

272200 OROBOROS^® Integrated Suction System - ISS

8000 OROBOROS^® TIP2k Titration-Injection microPump

·       With step motor and TIP microprocessor control unit.

·       New options for inhibitor titrations and kinetic studies.

·       For steady-state injection respirometry (0.01 - 25 µl.s^-1) and automatic titration regimes (0.05 - 250 µl).

·       Convenient mounting and exchange of Hamilton syringes.

·       TIP2k control integrated into DatLab 4.

·       Programmable titration volumes, number of steps and injection velocity.

·      Special length injection microneedle with gas bubble-free connection.

·      New with DatLat 4.3: Feedback control for oxy-stat or pH-stat applications.

29300 Oxygraph-2k Application Course and MiPNet Workshop

• Oxygraph-2k workshops on High-Resolution Respirometry are organized each year in an Alpine resort, three days.