OroboroPedia | Specific, Abbr.
Product | Definition Last update 2010-08-21 | Web
Link |
A | | | |
B | | | |
Barometric pressure | pb | Barometric pressure is measured by the O2k with an electronic pressure transducer and used to calculated the partial oxygen pressure in air satured with water vapour for air calibration of the POS. | |
Bench space for O2k | | The Oxygraph-2k requires a minimum of lab space (0.5 m bench space) besides an external PC or laptop, with sufficient space for syringes and handling. For highest signal stability and optimum temperature control, the O2k should be placed in a position that excludes exposure to direct sunlight and avoids suddent changes of room temperature. Keep the space below the Oxygraph-2k bottom clear, to prevent any interference of ventilation beneath the main unit. | MiPNet12.06 |
C | | | |
Ca++ electrode | 30510-24 | Ion sensitive electrode (ISE Ca++), 2 mm diamter shaft, for 30222-24 stopper, with separate reference electrode; combined into one BNC plug | |
Chamber holder for PVDF stopper | 23400-24 | Chamber holder (blue POM) for PVDF stopper, with O-ring (#23420-24) and V-ring (#23430-24). After the glass chamber is inserted into the copper block, the chamber holder is srewed on top of the chamber into the copper block to hold the chamber into a tight vertical position (O-ring) and prevent liquid to flow from the stainless steel housing into the space outside of the chamber (V-ring). This chamber holder has a configuration different from the one for the ►titanium stoppers. | |
Chamber holder for PVDF stopper basic | 23410-24 | Chamber holder made of blue POM, for application with PVDF stoppers. The titanium stoppers require a different chamber holder. | MiPNet12.06 |
Chamber label | | The chamber label of the O2k is by default A and B (left and right). In Datlab, this provides the option to label the left and right chambers. Chamber labels are used in file names (appended to the date), as an index of the quantities (plots of concentration and flux for the left and right chamber) and protocol. When operating more than a single O2k, it is recommended to label the chambers of the second O2k as C and D, etc. | MiPNet12.06 |
Chamber O2k | 23100-21 | Glass O2k-chamber, Ø 16 mm, duran polished, with standard operation volume of 2 ml. | MiPNet09.01 |
Chemical background correction of oxygen flux | | Correction for the side reaction of autooxidation, as a function of oxygen concentration. | MiPNet06.06 |
Cover slip | 24300-21 | The perspex cover slips should be placed on top of the stoppers to minimize contamination and evaporation of liquid extruding from the capillary of the stopper. The cover slips do not exert any direct effect on oxygen backdiffusion. | |
Cycles in TP2k program line | | Cycles is the number of times the injection is repeated with a pumping phase followed by a pause. | |
D | | | |
Data recording interval | | The time interval [s] for data recording can be set between 0.2 s and 10.0 s, always identical for both chambers. Rigorous tests revealed that data recording intervals <1 s do not increase the information. The default of 2 s is an optimum for most applications. Do not decrease the Data recording interval unless necessary, since at low Data recording intervals the apparent noise of flux is increased and on-line information is reduced. The data recording interval is displayed (read only); it is selected in the Oxygraph Control window [F7]. A short data sampling interval increases the time resolution and noise of flux, which may be compensated for by selecting a low value of N for flux derivation. | MiPNet12.06 |
DatLab 4.3 | 28001-43 | DatLab 4 Acquisition & Analysis software for ►high-resolution respirometry. | DatLab |
DatLab upgrading from 4.1 | 28002-43 | Upgrading DatLab 4.1 to 4.3, with free follow-up updates of DatLab 4.3, incl. O2k-Manual | DatLab |
Delay of TIP2k program | | Delay in the DatLab program which is the time [s] before starting the first instruction of any program line. | MiPNet09.10 |
Direct TIP2k control | | Operation mode for delivering a defined volume in a short pulse. A number of cycles, N, can be defined, and the duration of the interval from injection to injection determined, repeating the process N times. Several such steps can be programmed in DatLab to run in sequence to give an entire TIP program. | MiPNet09.10 |
E | | | |
Electrolyte powder POS | 26300-21 | ►POS. | |
F | | | |
Feedback TIP2k control | | Operation mode to reach and maintain a certain parameter (e.g. oxygen concentration) by automatic TIP injections, according to programmable DatLab setups. | MiPNet09.10 |
Forceps for membrane application | 51000-34 | Forceps for POS and ISE membrane application; not to be used for tissue preparation. | MiPNet08.04 |
Forceps sharp rounded tip | 51200-24 | Forceps for tissue preparation, stainless steel, antimagnetic, sharp rounded tip. One pair is recommended. | MiPNet14.14 |
Forceps sharp straight tip | 51100-24 | Forceps for tissue preparation, stainless steel, antimagnetic, sharp straight tip. One pair is recommended. | MiPNet14.14 |
Forceps very sharp angular tip | 51300-34 | Forceps for tissue preparation, stainless steel, antimagnetic, very sharp angular tip. Two pairs are recommended. | MiPNet14.14 |
Front limit of TIP2k | | When the front or rear limit of the pump is reached, the motor stops automatically. This status is indicated by the blinking green light on the rear push button. After a short push on the TIP button in the other direction, the TIP is in the status ready, the green light is on without blinking. | MiPNet09.10 |
G | | | |
Gain for POS | | The gain can be changed for signal amplification in specific cases. The default is a gain of 2 V/µA; options are 1, 2, 4, or 8 V/µA amplifications. In particular, use a lower gain if sections of the experiment are performed at high oxygen above air saturation, when the maximum voltage is close to 10 V or higher (offscale). Use a higher gain at low signal output when the maximum voltage obtained during an experiment is <3 V (e.g. at low temperature). | DatLab |
Gentle Science | GS | Gentle Science recognizes the special responsibility of the scientific community, for the quality of science, the quality of life in science, and its mission. While the individual scientist contributes her/his share, the scientific community requires concerted actions to encourage Gentle Science on institutional, national and world-wide levels. | http://www.oroboros.at/index.php?gentle-science |
H | | | |
Hamilton syringe 10 mm3 51/0.13 mm fixed | 40010-22 | Hamilton syringe for manual titrations, 10 mm3 volume; injection needle 51 mm length, 0.13 mm inner diameter. 6 syringes are recommended for standard applications. | MiPNet09.12 |
Hamilton syringe 100 mm3 51/0.41 mm fixed | 40100-24 | Hamilton syringe for manual titrations, 100 mm3 volume; injection needle 51 mm length, 0.41 mm inner diameter. These syringes are not included in our standard set, but are recommended for injections of suspensions of isolated mitochondria. | |
Hamilton syringe 25 mm3 51/0.15 mm fixed | 40025-22 | Hamilton syringe for manual titrations, 25 mm3 volume; injection needle 51 mm length, 0.15 mm inner diameter. 6 syringes are recommended for standard applications. | MiPNet09.12 |
Hamilton syringe 50 mm3 51/0.15 mm fixed | 40050-22 | Hamilton syringe for manual titrations, 50 mm3 volume; injection needle 51 mm length, 0.15 mm inner diameter. 2 syringes are recommended for standard applications. | MiPNet09.12 |
High-resolution respirometry | HRR | High-resolution respirometry is based on the OROBOROS Oxygraph-2k, combining chamber design, application of oxygen-tight materials, electrochemical sensors and electronics, Peltier-temperature control and software features (►DatLab) to obtain a unique level of quantitative resolution of oxygen concentration and oxygen flux, with a closed-chamber or open-chamber mode of operation (►TIP2k). Standardized two-point ►calibration of the polarographic oxygen sensor (static sensor calibration), calibration of the sensor response time (dynamic sensor calibration), and evaluation of ►instrumental background oxygen flux (systemic flux compensation) provide the experimental basis for high accuracy of quantitative results and quality control in HRR. | HRR |
I | | | |
Instrumental background oxygen flux | | Instrumental background oxygen flux in a respirometer is due to oxygen consumption by the POS, and oxygen diffusion into or out of the aqueous medium in the O2k-chamber. It is measured in the range of experimental oxygen levels by a standardized instrumental background test, and is a property of the instrumental system. Instrumental background correction eliminates errors by systemic flux compensation, automatically performed by DatLab. | MiPNet14.06 |
Instrumental background test | | Instrumental background tests were designed to detect and eliminate possible oxygen leaks, introducing this integrated systemic calibration as a key component of respirometric quality control. | MiPNet14.06 |
Integrated Suction System ISS 120 V | 27221-24 | Integrated Suction System (ISS), 120 V suction pump with stainless steel housing, 2-liter waste bottle, filter, and tubing; for siphoning off excess medium and for emptying the O2k chambers. | ISS |
Integrated Suction System ISS 230 V | 27220-24 | Integrated Suction System (ISS), 230 V suction pump with stainless steel housing, 2-liter waste bottle, filter, and tubing; for siphoning off excess medium and for emptying the O2k chambers. | ISS |
Interval in TIP programme | | Interval [s] is the total time from the start of a pumping cycle to the start of the consecutive cycle. It is the sum of the titration-injection (pumping) "Time [s]", and a pause between the end of the previous and the beginning of the following pumping state. | MiPNet09.10 |
IOC Workshop | | International Oxygraph Course, O2k-workshop | IOC |
Ion sensitive electrode | ISE | ►ISE. | ISE |
ISE | Ion sensitive electrode | The OROBOROS Ion Selective Electrode (ISE) has been developed for simultaneous monitoring of respiration and TPP+. The ISE system consists of separate reference and measuring electrodes. Typical examples are electrodes selective for hydrophobic cations (TPP+, TPMP+), Ca2+, Mg2+. | ISE |
ISE accessory box | 32900-01 | ISE accessory box | MiPNet15.03 |
ISE accessory set | 32200-01 | ISE accessory set | MiPNet15.03 |
ISE Ca membrane | 32202-01 | ISE Membrane: Oroboros Ca, 4 mm, package of 5 membranes | |
ISE compressible tube | 32103-01 | Compressible tube for ISE 32100-01 | MiPNet15.03 |
ISE consumables spares set | 32150-01 | ISE consumables spares set | MiPNet15.03 |
ISE electrolyte for reference electrode | 32802-01 | Electrolyte for reference electrode (32800-01) | MiPNet15.03 |
ISE filling syringe with needle | 32400-01 | Filling syringe with needle for ISE 32100-01 | MiPNet15.03 |
ISE inner glass electrode | 32101-01 | Inner glass electrode, with Ag/AgCl- and Pt-wire | MiPNet15.03 |
ISE membrane mounting tool | 32300-01 | ISE membrane mounting tool | MiPNet15.03 |
ISE membrane seal | 32102-01 | ISE membrane seal | MiPNet15.03 |
ISE Package 1 TPP, Ca | 32001-01 | Complete ISE Package (1 TPP+ Electrode). | |
ISE Package 2 TPP, Ca | 32000-01 | Complete ISE Package (2 TPP+ Electrodes). | |
ISE reference electrode 2.4 mm | 32800-01 | Reference electrode, 2.4 mm diamter glass shaft, for ion selective electrode, incl. 2 replacement barrels | MiPNet15.03 |
ISE replacement barrel for reference electrode | 32801-01 | Replacement barrel for reference electrode (32800-01) 2 mm glass | MiPNet15.03 |
ISE | 30500-24 | Ion sensitive electrode (pX), 70 mm shaft, for 30200-24 stopper | ISE |
ISE shaft for TPP, Ca | 32100-01 | 6 mm diameter shaft for OROBOROS Ion selective electrode (ISE TPP, Ca), for 30302-34 stopper | MiPNet15.03 |
ISE short needle | 32500-01 | Short needle, for bubble extrusion | MiPNet15.03 |
ISE TPP membrane | 32201-01 | ISE Membrane: Oroboros TPP, PVC, 4 mm, package of 5 membranes | MiPNet15.03 |
ISE TPP, Ca, reference | 30520-34 | Ion sensitive electrode (TPP+, Ca2+) for 30302-34 stopper, with separate reference electrode. | ISE |
ISS lid | 27230-24 | ►ISS; lid. | ISS |
ISS waste bottle | 27230-24 | Lid for 2-liter waste bottle of ►ISS; 3 holes for tubing connection. | ISS |
J | | | |
JO2 | | Oxygen flux, respiration expressed per unit system size, e.g. per volume or per mg wet weight. | |
K | | | |
L | | | |
M | | | |
Microbalance 120 g | XS105DU | 41 g / 120 g; 0.01 mg / 0.1 mg display. | |
Microbalance 220 g | XS205DU | 81 g / 220 g; 0.01 mg / 0.1 mg display. | |
Microbalance transport case | XS-Case | Microbalance transport case. | |
MiPArt Gallery | | The OROBOROS MiPArt Gallery forms a triangle connecting science, the scientific company OROBOROS INSTRUMENTS, and scientific art on the topic of mitochondrial physiology and links of science and art in general. | MiPArt |
MiPNet-Publications | | MiPNet is the abbreviation for the 'OROBOROS Journal 'Mitochondrial Physiol. Network', including chapters of the O2k-Manual, protocols, application notes, O2k-workshops, and other announcements, starting with MiPNet 1 in 1996. | ref-mipnet |
MiPNet-Reference Laboratories | | Mitochondrial physiology network, OROBOROS reference laboratories.
See also ►MiPNet-Publications. | Net |
MiPsociety | | Mitochondiral Physiology Society - www.mitophysiology.org | MiP |
MiR06 | | Mitochondrial respiration medium 06, developed for oxygraph incubations of mitochondrial preparations; MiR05 plus catalase | MiPNet14.13 |
MitoPedia | | Scientific terms and links in mitochondrial respiratory physiology. | MitoPedia |
MultiSensor connector for reference electrode | 30420-24 | MultiSensor connector for separate ref-electrodes, only for O2k Series B and C. | |
MultiSensor electronic upgrading | 30100-34 | Multisensor O2k MiP-NetAnalyzer: Electronic upgrading of the No. 20000-34 Oxygraph-2k (Series D) with additional 4 electronic channels (two potentiometric for pH, pX; including TPP+, Ca2+; two additional amperometric sensors for for NO, H2S) | |
MultiSensor electronic upgrading | 30101-34 | Multisensor O2k MiP-NetAnalyzer: Supplementary electronic upgrading of the No. 20000-34 Oxygraph-2k (Series D) with additional 4 electronic channels (two potentiometric for pH, pX; including TPP+, Ca2+; two additional amperometric sensors for for NO, H2S) for later extension. | |
MultiSensor products alphabetical | | All products available for the O2k-MultiSensor system in alphabetical order. | multisensor-a |
MultiSensor products numerical | | All products available for the O2k-MultiSensor system in numerical order. | multisensor-n |
MultiSensor stopper for ISE, PVDF angular, basic, 2.6 and 6.2 mm side ports | 30302-34 | PVDF stopper for multisensor application (ISE); side titration port and two additional holes (6.2 mm and 2.6 mm); angular bottom; with 4 spare Viton O-rings (12x1 mm), with volume calibration ring | MiPNet15.03 |
MultiSensor stopper for NO, conical, central and 2.3 mm side port | 30221-24 | MultiSensor PVDF stopper for MultiSensor applications (NO); 1 additional hole (2.3 mm); central titration port; conical bottom; with 4 spare Viton O-rings (12x1 mm), with volume calibration ring | |
MultiSensor stopper PEEK black conical, central and 2.5 mm side port | 31221-34 | PEEK Stopper for optical multisensor application; black (with PTFE, graphite, carbon fiber); 1 additional hole (2.5 mm); central titration port; conical bottom; with 4 spare Viton O-rings (12x1 mm; 2 mounted, 2 spare), with volume calibration ring | |
MultiSensor stopper PVDF shaft angular, central port | 24122-24 | PVDF stopper shaft, angular base, for stopper #30202-24. | |
MultiSensor stopper PVDF shaft conical, central port | 24121-24 | PVDF stopper shaft, with one central capillary and conical base, receptacle on the top for cellecting excess medium during closing the O2k-chamber and during titrations. | |
MultiSensor stopper, angular, basic and 5 mm side port | 30251-24 | MultiSensor PVDF Stopper for MultiSensor applications; 1 additional hole (5 mm); side titration port; angular bottom; with 4 spare Viton O-rings | |
MultiSensor stopper, angular, basic and 6 mm side port | 30262-24 | MultiSensor PVDF stopper for multisensor application; 1 additional hole (6 mm); side titration port; angular bottom; with 4 spare Viton O-rings (12x1 mm), with volume calibration ring | |
MultiSensor stopper, angular, optional ports | 30202-24 | MultiSensor PVDF stopper for MultiSensor applications; 24122-24 shaft with additional holes custom made
according to customer specification (max. 8 mm diameter); angular bottom; with 4 spare Viton O-rings (12x1 mm); with volume calibration ring | |
MultiSensor stopper, conical, optional ports | 30201-24 | MultiSensor PVDF stopper; 24121-24 shaft with additional holes custom made, according to customer specification (max. 3.5 mm diameter); conical bottom; with 4 spare Viton O-rings (12x1 mm); with volume calibration ring | |
Multiwell respirometer | | Multiwell systems are developed as a tool for qualitative high-throughput screening, for pharmacologic testing of a large number of substances. Designed for high-throughput qualitative measurements, they should not be advertised for quantitative measurements. Reliable quantitative results cannot be obtained with the present technology offered and advertised. | HRR-FAQ |
N | | | |
News | July 2010 | Selected page: www.mipart.at/index.php?oroboros-archetypical-symbol | July 2010 |
NO preamplifier | 30430-24 | NO Preamplifier, only for O2k Series B and C. | |
O | | | |
O2k | | ►Oxygraph-2k | O2k |
O2k box 1 | 20210-24 | Box 1 with the polystyrene inlets, for shipment of O2k main unit. Keep the original packing material safely stored, for any future shipping purposes of the O2k main unit. | |
O2k box 2 | 20220-24 | Box 2, for shipment of accessories. | |
O2k fuses power plug | 26700-21 | Fuses mains, T 2 A, 5x20 mm, spare. Until present, no record is available that a fuse had to be replaced on the socket for the mains power cable, at the rear panel of the O2k. Internal fuses on the electric circuits of the O2k can be exchanged only in our electronics workshop. | MiPNet12.06 |
O2k-accessory box | 27100-21 | Accessory box for the basic O2k, containing 2 stoppers, 2 glass chambers and 2 OROBoPOS for shipment, and accessories particularly for POS service. | MiPNet12.06 |
O2k-Publications | | Publication list of applications of the OROBOROS Oxygraph-2k and OROBOROS Oxygraph Paar. | O2k-publications |
O-Ring Viton 12x1 mm | 24110-24 | ►Viton O-ring (12x1 mm) for PVDF ►stopper, two are required per stopper, box of 4 as spares. | |
O-Ring Viton 18x2 mm | 23420-24 | ►Viton O-ring (18 x 2 mm), for ►chamber holder. | |
Oroboros | | Ouroboros or Uroboros, dragon biting its tail, archetypical symbol, particularly in alchemy, logo of OROBOROS INSTRUMENTS as a symbol for 'Cooperation and Feedback in Science'. | ORO |
OROBOROS INSTRUMENTS | | OROBOROS INSTRUMENTS - a new Quality in Science
Oxygraph-2k and O2k-MultiSensor MiPNetAnalyzer
unique for high-resolution respirometry - HRR
quantitative, non-plastic, scientific | Oroboros |
Oxygen flow | IO2 | Oxygen consumption per total system, ►extensive quantity. | |
Oxygen flux | JO2 | Oxygen consumption per system size, ►specific quantity. | MiPNet10.05 |
Oxygen pressure | pO2 | Partial pressure of oxygen [kPa], related to oxygen concentration in solution by the oxygen solubility, SO2 [µmol/kPa]. | MiPNet06.03 |
Oxygen solubility | SO2 | The oxygen solubility [µM/kPa] expresses the oxygen concentration in solution in equilibrium with the oxygen pressure in a gas phase, as a function of temperature and composition of the solution. SO2 is 10.56 µM/kPa in pure water at 37 °C. At standard barometric pressure (100 kPa), the oxygen concentration at air saturation is 207.3 µM at 37 °C (19.6 kPa partial oxygen pressure). In MiR06 and serum, the corresponding saturation concentrations are 191 and 184 µM. | MiPNet06.03 |
Oxygen solubility factor | FM | The oxygen solubility factor of the incubation medium, FM, expresses the effect of the salt concentration on oxygen solubility relative to pure water. In mitochondrial respiration medium MiR06, FM is 0.92 determined at 30 and 37 °C, and FM is 0.89 in serum at 37 °C. | MiPNet06.03 |
Oxygraph-2k | O2k | OROBOROS Oxygraph-2k (O2k) for high-resolution respirometry, the two-chamber high-resolution respirometer for monitoring oxygen consumption with small amounts of biological material, developed by OROBOROS INSTRUMENTS in cooperation with WGT, produced in Austria by WGT, and distributed world-wide by OROBOROS INSTRUMENTS. | O2k |
Oxygraph-2k compendium | 28000-43 | Oxygraph-2k compendium for high-resolution respirometry, including Oxygraph-2k User and Service Manual, CD with DatLab; O2k Literature, and info on O2k-Workshops. | |
Oxygraph-2k scientific support service | 29400-24 | Scientific support visit (3 days) by an O2k-application specialist provides scientific support in specific areas of applications, including help with sample preparation, design and testing of titration protocols (including ►SUIT protocols), and DatLab analysis. | |
Oxygraph-2k start-up service | 29250-24 | Setup and start-up visit: O2k training course (3 days). In most cases, the O2k-manual provides sufficient detail for setting up the O2k and starting measurements, particularly in conjunction with participation in an introductory O2k-►Workshop. For specific requirements, however, a start-up visit by a scientific application specialist supports a highly efficient start of a project. | |
P | | | |
PEEK | Polyether ether ketone | PEEK is a semicrystalline thermoplastic with excellent mechanical and chemical resistance properties that are retained to high temperatures, highly resistant to attack by both organic and aqueous environments, an advanced biomaterial used in medical implants. PEEK is used in the O2k for ►stirrer bars (an alternative is ►PVDF), and for the body of the ►POS. | |
Pen contact oil | 26550-21 | Pen contact oil, for stable low contact resistance between the ►POS head and the ►POS connector. | |
pH combination electrode with PVDF stopper | 33000-01 | pH combination electrode with PVDF stopper | |
pH electrode 150/6 mm | 30502-24 | pH combination electrode, 150 mm shaft, 6 mm diameter, incl. connection cable with BNC plug
for 30252-24 stopper | |
pH electrode 70/5 mm | 30501-24 | pH combination electrode, 70 mm shaft, 5 mm diameter, for 30251-24 stopper | |
Pipette for electrolyte | 26800-21 | Pipette in O2k-Accessory box, for filling electrolyte into the reservoir of the POS. | MiPNet12.06 |
Plunger 10 mm3 | 41010-21 | Spare plunger for 10 mm3 ►Hamilton syringe | |
Plunger 25 mm3 | 41025-21 | Spare plunger for 25 mm3 syringe | |
Plunger 50 mm3 | 41050-21 | Spare plunger for 50 mm3 syringe | |
Polarization voltage | | A constant polarization voltage is applied to a sensor operated in an amperometric mode (polarographic oxygen sensor; NO sensor). | POS |
Polarographic oxygen sensor | POS | Electrochemical oxygen sensor, introduced by L Clark, with application of a polarization voltage (0.6 to 0.8 V) and measurement of a current (amperometric), which is converted to a voltage. | POS |
POS connector and cable | 25000-34 | POS connector and cable connection. | MiPNet12.06 |
POS electrolyte powder | 26300-21 | POS Electrolyte powder, KCl | |
POS holder | 25100-21 | The POS holder is screwed into the copper block of the O2k, fixing the glass chamber in an accurate rotational position by pressing against the angular cut of the glass chamber. | MiPNet12.06 |
POS membrane holder ring | 26120-24 | POS membrane holder ring, PEEK, holds the membrane against the inner O-ring on the POS housing. For shipment in the O2k-accessory box, the POS is enclosed in a perspex housing. When the POS is uncsrewed from the blue base of the housing, the holder ring slides out from the POS shaft, and can be put in place with the ►mounting tool. | MiPNet08.04 |
POS membranes | 26200-21 | POS membranes, FEP 25 µm; 40/Pck. Two Pck. are in the O2k-accessory box. | MiPNet08.04 |
POS mounting tool | 26400-21 | POS mounting tool for membrane application, in the O2k-accessory box. | MiPNet08.04 |
POS O-ring 9x1 mm | 26130-21 | POS O-ring for sensor head or POS mounting tool, 9x1 mm | |
POS polishing cloth | 26510-21 | POS polishing cloth for cathode cleaning. | MiPNet08.04 |
POS polishing powder | 26520-21 | POS polishing powder for cathode cleaning. | MiPNet08.04 |
POS seal tip | 26110-21 | POS seal tip, butyl rubber gasket with 3 mm Ø pore. 4/Pck in O2k-accessory box, for sealing the POS head against the glass chamber. Push the wetted POS seal tip (black gasket) over the POS head, with the pore positioned centrally. Do not strech the gasket across the POS head. | MiPNet12.06 |
POS service | ## | POS connector service; replacement of cable and amplifyer in the OROBOROS electronics workshop (WGT). | |
Power cable O2k 120 V | 27311-21 | Main power cable for O2k - US (120 V) | MiPNet12.06 |
Power cable O2k 230 V | 27310-21 | Main power cable for O2k - EU (230 V) | MiPNet12.06 |
Products alphabetical | | All products available from OROBOROS INSTRUMENTS in alphabetical order. | O2k-alphabetical |
Products numerical | | All products available from OROBOROS INSTRUMENTS in numerical order. | O2k-numerical |
PVDF | polyvinylidene fluoride | ►Stopper; ►Stirrer bar. PVDF is used generally in applications requiring the highest purity, strength, and resistance to solvents, acids, bases. In the O2k, PVDF is used specifically due to its extremely low oxygen solubility, for minimization of oxygen backdiffusion into the O2k-chamber. | MiPNet06.01 |
Q | | | |
R | | | |
Rear limit of TIP2k | | When the front or rear limit of the pump is reached, the motor stops automatically. This status is indicated by the blinking green light on the rear push button. After a short push on the TIP button in the other direction, the TIP is in the status ready, the green light is on without blinking. | MiPNet09.10 |
Resume TIP programme | | Resume will continue a TIP setup halted with ►Suspend. If the scheduled time for an injection was reached during the time the TIP suspended this injection will start immediately after pressing Resume. | MiPNet09.10 |
RS232 cable | 27320-21 | RS232 cable for connecting the O2k main unit to the PC or laptop with DatLab installed. | MiPNet12.06 |
S | | | |
Stirrer bar PEEK black 15x6 mm | 23220-24 | PEEK stirrer bar, black, for specific applications with light guides to minimize optical interference. | |
Stirrer bar PVDF 15x6 mm | 23210-24 | PVDF stirrer bar, 15x6 mm, operated in the O2k-chamber at constant stirring speed (standard is 750 rpm, or 12.5 Hz), to provide optimum mixing of the sample in the aqueous medium and ensure a stable signal of the polarographic oxygen sensor placed in a position of maximum current of the medium. | MiPNet06.01 |
Stopper PVDF conical, central port | 24100-24 | PVDF stopper for closing the O2k-glass chamber, with one capillary and conical basis for basic O2k-applications; including volume calibration ring (A or B) for volume adjustment 1.5 to 3.2 ml; 4 spare O-rings (12x1 mm); requires #23400-24 chamber holder (different from chamber holder for titanium stoppers). | MiPNet12.06 |
Stopper spacer | 24200-24 | Stopper spacer (or gas spacer) to set the stopper into a standard position for a fixed gas phase above the aqueous phase in the O2k-chamber, during air calibration or for injection of nitrogen, argon or oxygen into the gas phase. | MiPNet14.06 |
Suspend TIP programme | | Suspend a TIP will halt the progress of the current program line if no injection is taking place at the moment. The time counter in the status line will continue to show elapsed time for the current program line, but no injection will be started. A running injection will NOT be stopped by Suspend. | |
Syringe 50 ml for gas injection | 42100-24 | Gas injection syringe, 50 ml, with spacer and stainless steel needle. | |
T | | | |
TIP event | | TIP events are set automatically into a plot in DatLab in line with a TIP operation. | MiPNet09.10 |
TIP setup | | A TIP setup is a predefined set of instructions to be executed by the TIP, plus informations about the used chemicals. The entire actual setup is displayed in a table (program lines table) in the lower part of the Control window. Each setup consists of at least one (but possible many) program lines. | MiPNet09.10 |
TIP2k and O2k-upgrading | 80100-24 | Titration-Injection microPump TIP2k; including electronic upgrading of O2k main unit | TIP2k-purchase |
TIP2k cooling box | 80400-22 | Cooling box for TIP2k syringes | |
TIP2k electronic upgrading | 80102-24 | Electronic upgrading of O2k main unit for TIP2k, making possible to add the TIP2k without requirement to send the O2k main unit to the OROBOROS workshop for upgrading. | TIP2k-purchase |
TIP2k product list | | All products available for the TIP2k. | tip2k-articles |
TIP2k spacer | 80600-24 | Spacers for microinjection TIP2k needle, silicone stops (200/Pkg.) with mounting tool | TIP2k-purchase |
TIP2k syringe 50 mm3 fixed | no sales | Microinjection syringe for TIP2k, 50 mm3,
with spacers | |
TIP2k syringe 500 mm3 fixed | 80500-24 | Microinjection syringe for TIP2k, 500 mm3,
with spacers | TIP2k-purchase |
TIP2k without electronic upgrading | 80101-24 | Titration-Injection microPump TIP2k; without electronic upgrading of O2k main unit | TIP2k |
Titration-Injection-microPump | TIP2k | Titration-Injection-microPump, for two-channel operation with the ►O2k. The TIP2k is placed at the top of the Oxygraph-2k. It does not need any extra bench space. Align the rear ends of the TIP2k and the Oxygraph-2k. This leaves space for a syringe cooling box. | TIP2k |
Trolley | 80800-xx | Aluminium trolley (700x500x60 mm); carrying capacity 120 kg; incl. packing box; for transport of O2k. | |
U | | | |
USB-RS232 serial adapter | 27330-24 | USB-RS232 Serial adapter, for connecting the RS232 cable attached to the O2k main unit to the USB port of the PC or laptop. This is not required when using a PC or laptop with a serial RS232 port. | MiPNet12.06 |
V | | | |
V, volume | | Volume of oxygraph chamber. | MiPNet08.04 |
Viton O-ring | | For ►O-rings, Viton is selected due to the low oxygen permeability, for minimization of oxygen backdiffusion into the O2k-chamber. | |
Volume calibration ring | 2413A-24 or 2413B-24 | Volume calibration ring (A or B) for PVDF Stoppers. If several O2k instruments are used, the chambers may be labelled (in DatLab) A, B, C, D, ... But volume calibration rings are available only with A and B. | MiPNet12.06 |
V-ring 30-35-4.5 mm | 23430-24 | V-ring (30/35/4.5 mm), for ►chamber holder. | MiPNet12.06 |
W | | | |
Wiki.Oroboros | | OroboroPedia and MitoPedia were started by OROBOROS INSTRUMENTS on 2010-07-12 as a glossary and index to our website, in an attempt to help the users finding particular topics more quickly, and to provide short definitions of terms, abbreviations and symbols frequently used in the context of high-resolution respirometry and mitochondrial physiology. OROBOROS INSTRUMENTS aims at open access and high scientific quality of information. This can be achieved only with feedback and input by contributions to OroboroPedia and MitoPedia, by the experienced users of the OROBOROS-O2k. If you find this page useful, we appreciate if you refer to OroboroPedia in the spirit of ►Gentle Science. | http://wiki.oroboros.at |
Workshop 3 days Standard | 29003-00 | Oxygraph-2k advanced MultiSensor Workshop (3 days), standard registration; participation in a previous introductory O2k-workshop is required. | Multi-Workshop |
Workshop 3 days Student | 29103-00 | Oxygraph-2k advanced MultiSensor Workshop (3 days), student registration; participation in a previous introductory O2k-workshop is required. | Multi-Workshop |
Workshop 4 days Standard | 29004-00 | Oxygraph-2k workshop; introductory training course
(4 Days), standard registration. | Workshop |
Workshop 4 days Student | 29104-00 | Oxygraph-2k workshop; introductory training course
(4 Days), student registration. | Workshop |
Workshop 7 days Standard | 29007-00 | Oxygraph-2k & O2k-Multisensor-Workshop (7 Days), standard registration; combined introductory and advanced MultiSensor workshop. | Workshop |
Workshop 7 days Student | 29107-00 | Oxygraph-2k & O2k-Multisensor-Workshop (7 Days), student registration; combined introductory and advanced MultiSensor workshop. | Workshop |
X | | | |
Y | | | |
Z | | | |
Zero solution dithionite | 26600-21 | Zero solution powder, ►dithionite, Na2S2O4, in O2k-accessory box. | |
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