68th International Workshop on High-Resolution Respirometry

2012 April 11 - 16, O2k-Workshop on High-Resolution Respirometry

Schroecken, Hotel Koerbersee

O2k-Basic and TPP-Basic




IOC68 Programme

1

Wednesday, April 11

 

 

Arrival

 

15:00 

Arrival in Bregenz: Meeting point Bregenz train station at 3:00 pm; approx. 1 hour bus drive to Schröcken and Hochtannberg (Salober).  Transfer/walk to Hotel Körbersee

 

18:30

Welcome reception at Hotel Körbersee.

 

19:00

Dinner

 

21:00

Get-together: Introduction of participants and their research interests

 

2

Thursday, April 12

 

 

Workshop 1

Weblink

 

Principles of high-resolution respirometry - from switching on the Oxygraph-2k to the experimental result

Gnaiger 2008 POS

08:00

Organize loan of skiing equipment

 

08:30-09:00

Erich Gnaiger: Get O2k-Connected with your OROBOROS-USB:
a guided tour to the O2k

get O2k-Connected

09:00-10:00

Instrumental quality control 1:
The oxygen sensor OROBoPOS -  calibration, stability testing, and evaluation of sensitivity to measure oxygen flux.

O2k-Calibration

10:00

Organize special lunch or bad weather task groups  (fibre and homogenate prep, fluorometry and spectrophotometry, NO, pH and Ca, Bioblast wiki login) and loan of skiing equipment

 

11:00

Practice - skiing, walk & talk, or individual O2k-tasks  /  Lunch

 

14:00

Coffee / Tea

 

14:30-16:00

O2k-Demo experiment 1:
Respiration of intact cells and on-line DatLab Analysis: Simultaneous measurement of oxygen consumption (O2k-Core) and H2O2 production (add-on O2k-Fluorescence LED2-Module)

O2k-Fluorometry Workshop

16:00

Coffee / Tea

 

16:30-17:15

DatLab Guide through the menues:
DL-Demo files and DL-Excel templates

DatLab 4 Guide

17:15-18:00

Mario Fasching: O2k-MultiSensor overview

 

18:30

Dinner

 

O2k-Basic
20:00-21:00

DatLab O2 flux analysis:
Flux per volume, flux per mass, flow per cell, flux control ratio

DatLab4 Flux Analysis

TPP-Basic
20:00-21:00

Hands-on: Assembly and maintenance of TPP and reference electrode

O2k-MultiSensor-Manual

3

Friday, April 13, 2012

 

 

Workshop 2

Weblink

O2k-Basic

O2k instrumental setup                OROBoPOS service

O2k-Start

08:30-09:15

Groups A and B                              Groups C and D

POS Service

09:15-10:00

Groups C and D                              Groups A and B

 

TPP-Basic
08:30-10:00

TPP-Basic: Hands on: Set up of the instrument with TPP and reference electrodes 

O2k-MultiSensor-Manual

10:00

Practice - skiing, walk & talk, or individual O2k-tasks  /  Lunch

 

14:00

Coffee / Tea

 

O2k-Basic
14:30-16:00

Instrumental quality control 2:
O2k-Background test and on-line analysis of oxygen flux.

O2k-Background

TPP-Basic
14:30-16:00

TPP-Basic: Introduction in Planning and Performing an TPP experiment;  Hands on: Instrumental background oxygen flux in the presence of the TPP electrode

O2k-TPP and Ca ISE-Module

16:00

Coffee / Tea

 

O2k-Basic
16:30-18:00

Hands-on (4 groups): O2k-Background from air saturation to zero oxygen concentration; or for permeabilized muscle fibres in the high-oxygen range of 500 - 200 µM.
A and B: O2k-Background with automatic TIP2k titration.
C and D: O2k-Background with manual titrations.

O2k-Background

TPP-Basic
16:30-18:00

TPP-Basic: Hands on: TPP calibration and TPP chemical background

TPP and HRR

18:30

Dinner

 

20:00-21:00

Hot MiP-Topics:  10+5 min presentations of abstracts by participants

 

4

Saturday, April 14, 2012

 

 

Workshop 3

Weblink

O2k-Basic
08:30-09:30

Experimental design 1:  Coupling control of mitochondrial respiration: LEAK, OXPHOS, ETS, ROX

Glossary: Respiratory states - Bioblast

O2k-Basic
09:30-10:00

Experimental design 2: Coupling Control Protocol with intact cells: ROUTINE, LEAK, ETS, ROX

Cells: PCP

TPP-Basic
08:30-10:00

From the TPP+ signal to mitochondrial membrane potential - Guide through the Excel templates.

 

10:00

Practice - skiing, walk & talk, or individual O2k-tasks  /  Lunch

 

14:00

Coffee / Tea

 

O2k-Basic
14:30-16.00

O2k-Demo experiment 2: SUIT protocol with myocardial homogenate and on-line DatLab analysis

Pesta 2012 Methods Mol Biol

TPP-Basic
14:30-16:00

Hands-on: TPP calibration and extending the TPP method for the determination of mitochondrial membrane potential beyond isolated mitochondria: TPP calibration and experiment with biological sample

 

16:00

Coffee / Tea

 

O2k-Basic
16:30-17:30

Hands-on: SUIT experiment continued with DatLab Analysis and guide through Excel templates

DatLab4 Flux Analysis

O2k-Basic
17:30-18:00

Carolina Doerrier and Andrea Eigentler: Mitochondrial respiration in permeabilized fibres versus homogenate from mouse myocardium. Application study with the PBI-Shredder

PBI-Shredder

TPP-Basic
16:30-18:00

Hands-on: continued, data evaluation

 

18:30

Dinner

 

O2k-Basic
20:00-21:00

Experimental design 3:
Substrate and coupling control of mitochondrial respiration.

CI+II

TPP-Basic
20:00-21:00

Hands-on: continued

 

5

Sunday, April 15, 2012

 

 

Workshop 4

Weblink

O2k-Basic
8:45-09:30

Working groups: Elaborate answers to the "Questions for the O2k-Course"

IOC-Questions

9:30-10:15

IOC-Questions - discussion of "Answers"

IOC-Questions

TPP-Basic
08:45-10:15

Final analysis of TPP experiment - discussion

 

10:15

Coffee / Tea

 

10:45-11:45

O2k-Demo experiment 3:  Respiration and steady-state feedback control of oxygen levels with the TIP2k.

TIP2k Manual

11:45-12:00

Barbara Meissner: The O2k-Workshop continues with the Bioblast wiki - MiPNet Reference Laboratories, O2k-Publications

http://www.bioblast.at

12:00

Lunch

 

13:00-13:45

Mario Fasching: Introduction into trouble shooting

 

13:45-14:45

Erich Gnaiger: MitoPathways through Complexes I+II - perspectives of comparative mitochondrial physiology

Gnaiger 2009 Int J Biochem Cell Biol

15:00

Snowshoe Walk (rental of snowshoes) to the Alpmuseum: Guided tour and reception: 15 €

www.alpmuseum.at

19:00

Dinner

 

20:30-21:00

Panel Discussion - Feedback IOC68

O2k-Feedback - Bioblast

 

Farewell party

 

6

Monday, April 16, 2012

 

 

Departure

 

 

Early morning: Departure

 



IOC68 Participants

Name

Lab  -  Fields of interest

Albertini Eva

AT_Innsbruck_Jansen Duerr P - Institute for Biomedical Aging Research, Austrian Academy Sciences.  -  FAHD1, senescence, aging, Mus musculus, C. elegans

Arbo Ingerid

NO_Trondheim_Rognmo O - Norwegian Univ Science and Technology.  -  Mitochondrial function, exercise training, disease

Bakkerud Fredrik

NO_Trondheim_Rognmo O - NTNU, Medical Faculty ISB.  -  Mitochondrial function, exercise training, disease

Barth Sonja

AT_Graz_Graier W - Dept Med Biochem Med Molec Biol, Univ Graz.  -  Calcium, endocannabinods

Bouitbir Jamal
(Hot topics IOC68-03)

CH_Basel_Kraehenbuehl S - Universitätsspital Basel, Dept Biomedizin.  -  ROS, statins, mitochondrial biogenesis, antioxidant capacity. TPP-Group

Cannon Daniel T.
(Hot topics IOC68-02)

US_CA Torrance_Rossiter HB - Inst Membrane Systems Biol, Fac Biol Sci, Univ Leeds, UK.  -  Exercise training, skeletal muscle, primary pulmonary arterial hypertension

Distefano Giovanna
(Hot topics IOC68-01)

US_PA Pittsburgh_Goodpaster B - Univ Pittsburgh, Div Endocrinol Metabolism, Dept Medicine, Cellular Rehabilitation Lab.  -  Aging, exercise, skeletal muscle, mitochondrial respiration

Doerrier Velasco Carolina A. (tutor)

ES_Granada_Acuna Castroviejo D - Ctr Investig Bioméd, Parque Tecnol Ciencias de la Salud, Univ Granada.

Eigentler Andrea (tutor)

AT_Innsbruck_Gnaiger E - DSL, Dept Visc, Transplant Thorac Surgery, Med Univ Innsbruck

Esfandiary Azadeh
(Hot topics IOC68-05)

DE_Giessen_Weissmann N - Justus-Liebig-Univ Giessen, Med Klinik II, Innere Medizin.  -  Mitochondria, cardiovascular diseases. TPP-Group

Fasching Mario (organizer, tutor)

AT_Innsbruck_OROBOROS INSTRUMENTS - TPP-Group

Felser Andrea

CH_Basel_Kraehenbuehl S - Universitätsspital Basel, Dept Biomed.  -  Mitochondria, ROS, apoptosis. TPP-Group

Fontana-Ayoub Mona (tutor)

AT_Innsbruck_OROBOROS INSTRUMENTS

Gnaiger Erich (organizer, tutor)

AT_Innsbruck_OROBOROS INSTRUMENTS; DSL, Dept Visceral, Transpl Thoracic Surgery, Medical Univ Innsbruck

Jespersen Nichlas Riise

DK_Aarhus_Jespersen NR - Univ Hospital Skejby.  -  Ischemia-reperfusion injury, respiratory capacity, ROS production

Johannsen Darcy

US_LA Baton Rouge_Noland RC - Pennington Biomed Res Ctr.  -  Respiration, human skeletal muscle, metabolism, chronic disease, obesity, type 2 diabetes

Kapitsinou Pinelopi

US_TN Nashville_Kapitsinou P - Vanderbilt Univ Med Ctr.  -  Hypoxia, signaling, endothelial cell bioenergetics

Kluckova Katarina

CZ_Prague_Neuzil J - Lab Molec Therapy, Inst Biotechnol, Acad Sci Czech Republic.  -  Cancer, inhibition, CI, CII

Lund Jim
(Hot topics IOC68-04)

NO_Tromso_Larsen TS - Cardiovasc Research Group, UiT, Univ Tromso.  -  Exercise, obesity, heart

Meers Grace

US_MO Columbia_Rector RS - VA Hospital.  -  Mitochondrial function, NAFLD

Meissner Barbara (tutor)

AT_Innsbruck_OROBOROS INSTRUMENTS

Noland Robert C.

US_LA Baton Rouge_Noland RC - Pennington Biomed Res Ctr.  -  Insulin resistance, diabetes, lipid and glucose metabolism, mitochondria, peroxisome, oxidation, oxygen consumption, membrane potential, ATP, oxidative stress

Ojuka Edward

ZA_Cape Town_Ojuka E - ESSM UCT Dept Human Biol Sports Sci, Inst South Africa Newlands, Univ Cape Town.  -  Nuclear respiratory factors

Olek Robert

PL_Gdansk_Kaczor JJ - Jedrzej Sniadecki Univ, School Phys Education Sport, Biochem Dpt.  -  Hypercaloric diets, mitochondrial function

Palladino Michael J.

US_PA Pittsburgh_Palladino MJ - Dpt Med-Pharmacol Chem Biol, Pittsburgh Inst Neurodegenerative Diseases (PIND), Univ Pittsburgh.  -  PC3, Drosophila

Peyta Laure

FR_Tours_Dumas JF - Inserm U1069 - N2C.  -  Hepatocytes, oxidative phosphorylation, ROS, membrane potential

Podramägi Taavi

EE_Tartu_Seppet EK - Univ Tartu.  -  TPP-Group

Rognmo Oivind

NO_Trondheim_Rognmo O - Fac Med, ISB, Norwegian Univ Sci Technol.  -  TPP-Group

Schmitz Lysann

DE_Cologne_Larsson NG - Max Planck Inst Biol Ageing.  -  OXPHOS dysfunction

Stottrup Nicolaj B.

DK_Aarhus_Jespersen NR - Univ Hospital Skejby.  -  Ischemia-reperfusion injury, respiratory capacity, ROS production

Uusijärvi Johan

SE_Stockholm_Weitzberg E - Karolinska Hospital, Dept Pharmacol Physiol.  -  Cyanide, hydroxicobalamine, sodiumthiosulfate

Woodlief Tracey

US_PA Pittsburgh_Goodpaster B - Univ Pittsburgh, Div Endocrinol Metabolism, Montefiore Hospital.  -  Lipid induced ROS production

Ying Li

SG_Singapore_Summer S - Dept Cardiovasc Metabolism Diseases, Duke-NUS Medical School.  -  Metabolic diseases, cancer, neuroscience

Ziolkowski Wieslaw

PL_Gdansk_Kaczor JJ - Jedrzej Sniadecki Univ, Sch Physical Education Sport, Biochem Dpt.  -  Exercise, skeletal and cardiac mitochondria, dystrophy.  TPP-Group

 

 




 

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