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68th International Workshop on High-Resolution Respirometry2012 April 11 - 16, O2k-Workshop on High-Resolution Respirometry
Schroecken, Hotel Koerbersee
O2k-Basic and TPP-Basic
IOC68 Programme1 | Wednesday, April 11 | | | Arrival | | 15:00 | Arrival in Bregenz: Meeting point Bregenz train station at 3:00 pm; approx. 1 hour bus drive to Schröcken and Hochtannberg (Salober). Transfer/walk to Hotel Körbersee | | 18:30 | Welcome reception at Hotel Körbersee. | | 19:00 | Dinner | | 21:00 | Get-together: Introduction of participants and their research interests | | | | | 2 | Thursday, April 12 | | | Workshop 1 | Weblink | | Principles of high-resolution respirometry - from switching on the Oxygraph-2k to the experimental result | Gnaiger 2008 POS | 08:00 | Organize loan of skiing equipment | | 08:30-09:00 | Erich Gnaiger: Get O2k-Connected with your OROBOROS-USB:
a guided tour to the O2k | get O2k-Connected | 09:00-10:00 | Instrumental quality control 1:
The oxygen sensor OROBoPOS - calibration, stability testing, and evaluation of sensitivity to measure oxygen flux. | O2k-Calibration | 10:00 | Organize special lunch or bad weather task groups (fibre and homogenate prep, fluorometry and spectrophotometry, NO, pH and Ca, Bioblast wiki login) and loan of skiing equipment | | 11:00 | Practice - skiing, walk & talk, or individual O2k-tasks / Lunch | | 14:00 | Coffee / Tea | | 14:30-16:00 | O2k-Demo experiment 1:
Respiration of intact cells and on-line DatLab Analysis: Simultaneous measurement of oxygen consumption (O2k-Core) and H2O2 production (add-on O2k-Fluorescence LED2-Module) | O2k-Fluorometry Workshop | 16:00 | Coffee / Tea | | 16:30-17:15 | DatLab Guide through the menues:
DL-Demo files and DL-Excel templates | DatLab 4 Guide | 17:15-18:00 | Mario Fasching: O2k-MultiSensor overview | | 18:30 | Dinner | | O2k-Basic
20:00-21:00 | DatLab O2 flux analysis:
Flux per volume, flux per mass, flow per cell, flux control ratio | DatLab4 Flux Analysis | TPP-Basic
20:00-21:00 | Hands-on: Assembly and maintenance of TPP and reference electrode | O2k-MultiSensor-Manual | | | | 3 | Friday, April 13, 2012 | | | Workshop 2 | Weblink | O2k-Basic | O2k instrumental setup OROBoPOS service | O2k-Start | 08:30-09:15 | Groups A and B Groups C and D | POS Service | 09:15-10:00 | Groups C and D Groups A and B | | TPP-Basic
08:30-10:00 | TPP-Basic: Hands on: Set up of the instrument with TPP and reference electrodes | O2k-MultiSensor-Manual | 10:00 | Practice - skiing, walk & talk, or individual O2k-tasks / Lunch | | 14:00 | Coffee / Tea | | O2k-Basic
14:30-16:00 | Instrumental quality control 2:
O2k-Background test and on-line analysis of oxygen flux. | O2k-Background | TPP-Basic
14:30-16:00 | TPP-Basic: Introduction in Planning and Performing an TPP experiment; Hands on: Instrumental background oxygen flux in the presence of the TPP electrode | O2k-TPP and Ca ISE-Module | 16:00 | Coffee / Tea | | O2k-Basic
16:30-18:00 | Hands-on (4 groups): O2k-Background from air saturation to zero oxygen concentration; or for permeabilized muscle fibres in the high-oxygen range of 500 - 200 µM.
A and B: O2k-Background with automatic TIP2k titration.
C and D: O2k-Background with manual titrations.
| O2k-Background | TPP-Basic
16:30-18:00 | TPP-Basic: Hands on: TPP calibration and TPP chemical background | TPP and HRR | 18:30 | Dinner | | 20:00-21:00 | Hot MiP-Topics: 10+5 min presentations of abstracts by participants | | | | | 4 | Saturday, April 14, 2012 | | | Workshop 3 | Weblink | O2k-Basic
08:30-09:30 | Experimental design 1: Coupling control of mitochondrial respiration: LEAK, OXPHOS, ETS, ROX | Glossary: Respiratory states - Bioblast | O2k-Basic
09:30-10:00 | Experimental design 2: Coupling Control Protocol with intact cells: ROUTINE, LEAK, ETS, ROX | Cells: PCP | TPP-Basic
08:30-10:00 | From the TPP+ signal to mitochondrial membrane potential - Guide through the Excel templates. | | 10:00 | Practice - skiing, walk & talk, or individual O2k-tasks / Lunch | | 14:00 | Coffee / Tea | | O2k-Basic
14:30-16.00 | O2k-Demo experiment 2: SUIT protocol with myocardial homogenate and on-line DatLab analysis | Pesta 2012 Methods Mol Biol | TPP-Basic
14:30-16:00 | Hands-on: TPP calibration and extending the TPP method for the determination of mitochondrial membrane potential beyond isolated mitochondria: TPP calibration and experiment with biological sample | | 16:00 | Coffee / Tea | | O2k-Basic
16:30-17:30 | Hands-on: SUIT experiment continued with DatLab Analysis and guide through Excel templates | DatLab4 Flux Analysis | O2k-Basic
17:30-18:00 | Carolina Doerrier and Andrea Eigentler: Mitochondrial respiration in permeabilized fibres versus homogenate from mouse myocardium. Application study with the PBI-Shredder | PBI-Shredder | TPP-Basic
16:30-18:00 | Hands-on: continued, data evaluation | | 18:30 | Dinner | | O2k-Basic
20:00-21:00 | Experimental design 3:
Substrate and coupling control of mitochondrial respiration. | CI+II | TPP-Basic
20:00-21:00 | Hands-on: continued | | | | | 5 | Sunday, April 15, 2012 | | | Workshop 4 | Weblink | O2k-Basic
8:45-09:30 | Working groups: Elaborate answers to the "Questions for the O2k-Course" | IOC-Questions | 9:30-10:15 | IOC-Questions - discussion of "Answers" | IOC-Questions | TPP-Basic
08:45-10:15 | Final analysis of TPP experiment - discussion | | 10:15 | Coffee / Tea | | 10:45-11:45 | O2k-Demo experiment 3: Respiration and steady-state feedback control of oxygen levels with the TIP2k. | TIP2k Manual | 11:45-12:00 | Barbara Meissner: The O2k-Workshop continues with the Bioblast wiki - MiPNet Reference Laboratories, O2k-Publications | http://www.bioblast.at | 12:00 | Lunch | | 13:00-13:45 | Mario Fasching: Introduction into trouble shooting | | 13:45-14:45 | Erich Gnaiger: MitoPathways through Complexes I+II - perspectives of comparative mitochondrial physiology | Gnaiger 2009 Int J Biochem Cell Biol | 15:00 | Snowshoe Walk (rental of snowshoes) to the Alpmuseum: Guided tour and reception: 15 € | www.alpmuseum.at | 19:00 | Dinner | | 20:30-21:00 | Panel Discussion - Feedback IOC68 | O2k-Feedback - Bioblast | | Farewell party | | | | | 6 | Monday, April 16, 2012 | | | Departure | | | Early morning: Departure | |
IOC68 ParticipantsName | Lab - Fields of interest | Albertini Eva | AT_Innsbruck_Jansen Duerr P - Institute for Biomedical Aging Research, Austrian Academy Sciences. - FAHD1, senescence, aging, Mus musculus, C. elegans | Arbo Ingerid | NO_Trondheim_Rognmo O - Norwegian Univ Science and Technology. - Mitochondrial function, exercise training, disease | Bakkerud Fredrik | NO_Trondheim_Rognmo O - NTNU, Medical Faculty ISB. - Mitochondrial function, exercise training, disease | Barth Sonja | AT_Graz_Graier W - Dept Med Biochem Med Molec Biol, Univ Graz. - Calcium, endocannabinods | Bouitbir Jamal
(Hot topics IOC68-03) | CH_Basel_Kraehenbuehl S - Universitätsspital Basel, Dept Biomedizin. - ROS, statins, mitochondrial biogenesis, antioxidant capacity. TPP-Group | Cannon Daniel T.
(Hot topics IOC68-02) | US_CA Torrance_Rossiter HB - Inst Membrane Systems Biol, Fac Biol Sci, Univ Leeds, UK. - Exercise training, skeletal muscle, primary pulmonary arterial hypertension | Distefano Giovanna
(Hot topics IOC68-01) | US_PA Pittsburgh_Goodpaster B - Univ Pittsburgh, Div Endocrinol Metabolism, Dept Medicine, Cellular Rehabilitation Lab. - Aging, exercise, skeletal muscle, mitochondrial respiration | Doerrier Velasco Carolina A. (tutor) | ES_Granada_Acuna Castroviejo D - Ctr Investig Bioméd, Parque Tecnol Ciencias de la Salud, Univ Granada. | Eigentler Andrea (tutor) | AT_Innsbruck_Gnaiger E - DSL, Dept Visc, Transplant Thorac Surgery, Med Univ Innsbruck | Esfandiary Azadeh
(Hot topics IOC68-05) | DE_Giessen_Weissmann N - Justus-Liebig-Univ Giessen, Med Klinik II, Innere Medizin. - Mitochondria, cardiovascular diseases. TPP-Group | Fasching Mario (organizer, tutor) | AT_Innsbruck_OROBOROS INSTRUMENTS - TPP-Group | Felser Andrea | CH_Basel_Kraehenbuehl S - Universitätsspital Basel, Dept Biomed. - Mitochondria, ROS, apoptosis. TPP-Group | Fontana-Ayoub Mona (tutor) | AT_Innsbruck_OROBOROS INSTRUMENTS | Gnaiger Erich (organizer, tutor) | AT_Innsbruck_OROBOROS INSTRUMENTS; DSL, Dept Visceral, Transpl Thoracic Surgery, Medical Univ Innsbruck | Jespersen Nichlas Riise | DK_Aarhus_Jespersen NR - Univ Hospital Skejby. - Ischemia-reperfusion injury, respiratory capacity, ROS production | Johannsen Darcy | US_LA Baton Rouge_Noland RC - Pennington Biomed Res Ctr. - Respiration, human skeletal muscle, metabolism, chronic disease, obesity, type 2 diabetes | Kapitsinou Pinelopi | US_TN Nashville_Kapitsinou P - Vanderbilt Univ Med Ctr. - Hypoxia, signaling, endothelial cell bioenergetics | Kluckova Katarina | CZ_Prague_Neuzil J - Lab Molec Therapy, Inst Biotechnol, Acad Sci Czech Republic. - Cancer, inhibition, CI, CII | Lund Jim
(Hot topics IOC68-04) | NO_Tromso_Larsen TS - Cardiovasc Research Group, UiT, Univ Tromso. - Exercise, obesity, heart | Meers Grace | US_MO Columbia_Rector RS - VA Hospital. - Mitochondrial function, NAFLD | Meissner Barbara (tutor) | AT_Innsbruck_OROBOROS INSTRUMENTS | Noland Robert C. | US_LA Baton Rouge_Noland RC - Pennington Biomed Res Ctr. - Insulin resistance, diabetes, lipid and glucose metabolism, mitochondria, peroxisome, oxidation, oxygen consumption, membrane potential, ATP, oxidative stress | Ojuka Edward | ZA_Cape Town_Ojuka E - ESSM UCT Dept Human Biol Sports Sci, Inst South Africa Newlands, Univ Cape Town. - Nuclear respiratory factors | Olek Robert | PL_Gdansk_Kaczor JJ - Jedrzej Sniadecki Univ, School Phys Education Sport, Biochem Dpt. - Hypercaloric diets, mitochondrial function | Palladino Michael J. | US_PA Pittsburgh_Palladino MJ - Dpt Med-Pharmacol Chem Biol, Pittsburgh Inst Neurodegenerative Diseases (PIND), Univ Pittsburgh. - PC3, Drosophila | Peyta Laure | FR_Tours_Dumas JF - Inserm U1069 - N2C. - Hepatocytes, oxidative phosphorylation, ROS, membrane potential | Podramägi Taavi | EE_Tartu_Seppet EK - Univ Tartu. - TPP-Group | Rognmo Oivind | NO_Trondheim_Rognmo O - Fac Med, ISB, Norwegian Univ Sci Technol. - TPP-Group | Schmitz Lysann | DE_Cologne_Larsson NG - Max Planck Inst Biol Ageing. - OXPHOS dysfunction | Stottrup Nicolaj B. | DK_Aarhus_Jespersen NR - Univ Hospital Skejby. - Ischemia-reperfusion injury, respiratory capacity, ROS production | Uusijärvi Johan | SE_Stockholm_Weitzberg E - Karolinska Hospital, Dept Pharmacol Physiol. - Cyanide, hydroxicobalamine, sodiumthiosulfate | Woodlief Tracey | US_PA Pittsburgh_Goodpaster B - Univ Pittsburgh, Div Endocrinol Metabolism, Montefiore Hospital. - Lipid induced ROS production | Ying Li | SG_Singapore_Summer S - Dept Cardiovasc Metabolism Diseases, Duke-NUS Medical School. - Metabolic diseases, cancer, neuroscience | Ziolkowski Wieslaw | PL_Gdansk_Kaczor JJ - Jedrzej Sniadecki Univ, Sch Physical Education Sport, Biochem Dpt. - Exercise, skeletal and cardiac mitochondria, dystrophy. TPP-Group |
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