IOC55/56 O2k- and MultiSensor Workshop on High-Resolution Respirometry

Schröcken - Körbersee


2010, April 7-15, IOC55 & 56, Introductory and MultiSensor O2k-Workshops on High-Resolution Respirometry. Hotel Körbersee, Schröcken, Austria.


Table of Contents

IOC55/ 56 - Feedback


  • "Thank you for your hospitality and all of the great times I had both in and out of Hotel Koerbersee.  It was a 3X in a lifetime experience.  I shall return. All the best to you and your team.  You really have an impressive organization and team to be proud of. Your experience and the education you provide at the workshops is unparalleled." - Robb Flynn, Nashville, USA
  • "Many thanks for the excellent workshop." - Vilma Borutaite, Kaunas, Lithuania
  • "I want to thank you so much for your kindness, reception and availability during the O2k workshop. It was a real pleasure to meet you and your team. Very good memories." – Céline Wetterwald, Angers, FR

  • "Thank you for the possiblity to spend so fantastic time among OROBOROS Team and excellent participants from other countries. I have learnt a lot!"
  • "I really enjoyed Erich´s speech... I feel that the topics were well covered!"
  • "I would like to thank you again for the very instructive days in Schröcken!"
  • "Thank you for your help and hospitality. I enjoyed the course!"
  • "A really, really great team, which was very supporting."

IOC55/56 - Lecturers and Tutors


OROBOROS INSTRUMENTS, Austria; and Med. Univ. Innsbruck, Dept. General Transplant Surgery, D. Swarovski Research Lab., Innsbruck, Austria

  • Erich Gnaiger, PhD, erich.gnaiger(at) 
  • Dominik Pesta, MSc, dominik.pesta(at)
  • Kathrin Renner-Sattler, PhD, kathrin.renner(at)
  • Zuzana Sumbalová, PhD, zuzana.sumbalova(at)
  • Anita Wiethüchter, MSc, anita.wiethuechter(at) (administration)

Invited tutors IOC55

  • Esther Phielix, German Diabetes Center, Heinrich-Heine-University Düsseldorf, Germany

Invited speakers IOC56

  • Vilma Borutaite, PhD, Institute for Biomedical Research, Kaunas University of Medicine, Lithuania

We continue our tradition of inviting international experts from MiPNet Reference Laboratories, extending the O2k-Team of international tutors.


IOC55/56 - Participants

  • Bliksøen Marte, PhD, IEMR Oslo University Hospital, Norway. (ischemia reperfusion injury, heart, gender differences) - IOC55

  • Borutaite Vilma, Prof. PhD, Institute for Biomedical Research, Kaunas University of Medicine, Lithuania. (invited speaker) - IOC56

  • Burgstaller Wolfgang, Prof. PhD, Institute of Microbiology, University of Innsbruck, Austria. (alternative respiration in filamentous fungi) - IOC55

  • Contreras Karla, PhD, Monash University, Division of Biological Engineering, Clayton, Australia. (respiration in stem cells and mammalian cells) - IOC55

  • Cortez Erika, PhD, Laboratory of Physiology of Nutrition and Development, Department of Physiological Sciences Federal University of Rio de Janeiro, Brazil. (changes in energy metabolism of lymphocytes as biomarkers of nutritional interventions effects) - IOC55/56

  • Davies Stefan, PhD, University of Western Australia, Institute for Medical Research, Perth, Australia.  (role of new nuclear-encoded mt proteins in cells and isolated mitochondria; bioenergetics, mt gene expression) - IOC55/56

  • Dzialowski M. Edward, PhD, Department of Biological Sciences, University of North Texas, Denton, USA. (developmental changes in mitochondria function) - IOC56

  • Eckert Schamim, Institute of Pharmacology, Frankfurt, Germany. (mt dysfunction in neurodegenerative diseases; investigating the function of the respiratory chain complexes – with and without challenge – by the O2-consumption) - IOC56

  • Flynn Robb, PhD, Vanderbilt University Medical Center, Department of Surgical Sciences, Nashville, USA. (non-alcoholic fatty liver disease; altered expression of mt proteins involved with fat oxidation and accumulation of lipid oxidation intermediates implicating multiple impairments in mt function) - IOC55

  • Galina Antonio, Prof. PhD, Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Brazil. (multisensor of membrane potential of DY, NO, pH and O2) - IOC56

  • Gnaiger Erich, A.Univ.-Prof. PhD, D. Swarovski Research Laboratory, Dept. General Transplant Surgery, Medical University Innsbruck; OROBOROS INSTRUMENTS; Austria. (organizer, tutor) - IOC55/56

  • Gonçalves Renata de Lima Sales, PhD, Institute of Biomedical Medicine, Federal University of Rio de Janeiro, Brazil. (linking the mt metabolism and mosquito immune response, pointing the regulation of mt ROS as an effective immune tool for A. gambiae against Plasmodium infection; mitochondria, flight muscle, vector disease and hematophagous) - IOC55/56

  • Habets Daphna, Department of Clinical Genetics, Maastricht University Hospital, Netherlands. (respiration in cultured fibroblasts and isolated mitochondria of patients with defects in the mt respiratory chain; diagnostics, metabolic inborn error) - IOC55

  • Jacobs Robert, Institute of Veterinary Physiology, University of Zurich, Switzerland. (regulation of mt respiration in response to hypoxia and/or exercise in skeletal muscle and adipose tissue; hypoxia, oxidative capacity, mt biogenesis, skeletal muscle, adipose tissue) - IOC55

  • Janke Linda, PhD, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University, Düsseldorf, Germany. (comparison of type 1 diabetes and type 2 diabetes in mice; interaction between insulin resistance and mitofunction, diabetes, permeabilized muscle fibers, mouse models) - IOC55/56

  • Kuncová Jitka, PhD, Faculty of Medicine in Plzen, Charles University in Prague, Czech Republic. (mt function in sepsis and chronic renal failure) - IOC56

  • Labieniec Magdalena, PhD, Department of General Biophysics, University of Lodz, Poland. - IOC55

  • Lanza Ian R., PhD, Mayo Clinic, Rochester, USA. (mt function in aging, exercise and nutrition) - IOC56

  • Lundby Carsten, Prof. PhD, Center for Integrative Human Physiology, University of Zurich, Switzerland. - IOC55

  • Paglialunga Sabina, Department of Human Biology, Maastricht University, The Netherlands. - IOC55/56

  • Pesta Dominik, Mag, D. Swarovski Research Laboratory, Dept. General Transplant Surgery, Medical University Innsbruck; and OROBOROS INSTRUMENTS; Austria. (tutor)

  • Phielix Esther, German Diabetes Center, Heinrich-Heine-University Düsseldorf, Germany. (tutor) - IOC55; (membrane potential and ROS production in permeabilized muscle fibres) - IOC56

  • Rae Epp, PhD, University of Tartu, Estonia. (experiments with skinned cardiac fibers at different oxygen level) - IOC55

  • Rasmussen Peter, PhD, Center for Integrative Human Physiology, University of Zurich, Switzerland. - IOC55

  • Renner-Sattler Kathrin, PhD, OROBOROS MiPNet, Regensburg, Germany.  (lecturer, tutor) - IOC55/56

  • Salvadego Desy, Department of Biomedical Sciences and Technologies, University of Udine, Italy. (peripheral limitations to oxidative metabolism in body builders; maximal oxygen consumption, mt activity, knee-extension exercise) - IOC55

  • Schiffer Tomas, PhD, Karolinska Institute, Swedish School of Sport and Health Sciences, Stockholm, Sweden. (nitric oxide, bioenergetics, exercise physiology) - IOC56

  • Schneider Maren, Cell Biology & Molecular Aging Research, Environmental Health Research Institute at the Heinrich-Heine-University, Duesseldorf, Germany. (respiratory measurements after induced stress in cultured cells) - IOC55

  • Schreilechner Anna, PhD, Department of Medical Biochemistry & Medical Molecular Biology, University of Graz, Austria. (respiratory oxygen consumption in cells and tissue and isolated mitochondria) - IOC55

  • Senft Katharina, University of Cologne, CECAD Cologne-Excellent in Aging Research at the Institute for Genetics, Cologne, Germany. (mt dysfunction in aging-associated diseases) - IOC55

  • Siebenmann Christoph, PhD, Institute of Physiology, University of Zurich, Switzerland. - IOC55

  • Stensløkken Kåre-Olav, Post Doc, Institute of Molecular Biosciences, University of Oslo, Norway. (border between comparative physiology and medical physiology; heart and ischemia reperfusion injury in mammalian hearts and the study of an anoxia tolerant fish, the crucian carp) - IOC55

  • Stepto Nigel, PhD, School of Sports and Exercise Science, Centre for Aging, Rehabilitation, Exercise and Sport (CARES), Melbourne, Australia. (mt function in skeletal muscle in humans; role of adaptation to exercise for health and performance; polycystic ovary syndrome, insulin resistance, women´s health; training and altitude interventions) - IOC55/56

  • Sumbalová Zuzana, PhD, OROBOROS INSTRUMENTS, Innsbruck, Austria. (tutor) - IOC55/56

  • Szendrödi Julia, PhD, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University, Düsseldorf, Germany. (insulin resistance of mitochondria) - IOC56

  • Van Bergen Nicole, Glaucoma Research Laboratory, Centre for Eye Research Australia, Melbourne, Australia. (mt dysfunction in cultured cells and isolated mitochondria of patients with defects in the mt respiratory chain, mt dysfunction in aging, mt biogenesis; mitochondrial dysfunction in optic neuropathies) - IOC55/56

  • Vieira Beiral Hellen Jan, PhD, Department of Biophysics and Physiology, Federal University of Rio de Janeiro, Brazil. - IOC55/56

  • Votyakova Tatyana, PhD, Children's Hospital of Pittsburgh of UPMC, USA. (basic mechanisms of ROS generation by mitochondria and mt mechanisms related to the pathologies of diabetes; monitoring respiratory activities of isolated beta-cells from various sources in order to relate this parameter with the cells wellbeing and suitability for transplantation) - IOC56

  • Vrabl Pamela, PhD, University of Innsbruck, Institute of Microbiology, Austria. (alternative respiration in filamentous fungi) - IOC55

  • Watala Cezary, Prof. PhD, Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz, Poland. - IOC55

  • Wetterwald Céline, PhD, Lab Biochim, Centre Hospitalier Univ. d'Angers, France. (mt pathologies: biochemical diagnosis, research on mt neuropathies; development of complementary analyses using TPP+, Ca2+ electrodes) - IOC56

  • Wiethüchter Anita, MSc, OROBOROS INSTRUMENTS, Innsbruck, Austria. (scientific coordinator) - IOC55/56

Programme - Advanced O2k-MultiSensor Workshop


Day 1: Monday, 12. April

15:00 Participants arriving in Bregenz:  Meeting point at 3:00 pm in Bregenz train station; 1.1 hour drive to Schröcken. Transfer and check in at Hotel Körbersee.

18:30 Welcome reception

19:00  Dinner

21:00-21:20 Erich Gnaiger (Innsbruck, AT) Expectations and reality in MutiSensor high-resolution respirometry.

21:20-22:00 Introduction of participants and their research interests.

Day 2: Tuesday, 13. April

8:30 – 10:00  Mario Fasching, Erich Gnaiger, Kathrin Renner-Sattler: Introduction to MultiSensor methodologies and applications with permeabilized cells. The TPP+ electrode – an example for ion selective electrodes.

10:00 Coffee

10:30 –12:00 Demo experiment: Calibration of the TPP+ electrode, and instrumental oxygen background in the presence of additional sensors

12:00 - 14:30  Lunch break

14:30 -18:30 Parallel group sessions: Hands-on O2k and TPP+

19:00 Dinner

21:00  Hot topics: MiPNet Session

Day 3: Wednesday, 14. April

08:30 – 10:00  Erich Gnaiger (Innsbruck, AT) Metabolic steady states: from cytochrome redox states and respiration to mt-membrane potential

10:00  Coffee

10:30 – 12:00 Vilma Borutaite (Kaunas, Lithuania): Ion distribution and mitochondrial membrane potential – theory.

12:00 – 14:00 Lunch break

14:00 -16:30 Mario Fasching, Zuzana Sumbalová: From the TPP+ signal to mitochondrial membrane potential. DatLab 3 and EXCEL analysis templates.

16:30  Coffee

17:00 – 18:30           Open topics:            

A. Workpackage pH:  setup of O2k with pH electrodes, volume calibration demo, pH calibration, electrode maintenance; application of the pH electrode: pH measurements in weak buffer, buffering capacity and experimental design.

B. Workpackage NO: electrode assembly, maintenance, calibration of the ion selective electrode; open chamber electrode calibration; blank experiment, data analysis.

C. Oxygen kinetics / steady state experiment

19:00  Dinner

21:00 IOC56 feedback - discussion - summary - conclusions

Day 4: Thursday, 15. April


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OROBOROS High-Resolution Respirometry