IOC53 International Workshop on High-Resolution Respirometry

 

30 July - 4 August 2009, 53rd IOC - International Course on High-Resolution Respirometry. Hotel Tannberg, Schroecken, Vorarlberg, Austria. 

The 53rd O2k-Course is the 20th presentation of high-resolution respirometry (HRR) in Schröcken since 1988, for the first time extended to four full days. The O2k-Course includes experiments with biological samples, providing a practical overview of the Oxygraph-2k, with integrated on-line analysis by DatLab 4.3 (new upgrade), applications of the TIP-2k, and perspectives of high-resolution respirometry in mitochondrial physiology. Emphasis is placed on hands-on applications by all participants.

        Experienced tutors guide small working groups step-by-step through the approach of HRR. Five Oxygraph-2k (10 chambers) are available for a do-it-yourself application of both hardware and software.  Combined with an introduction and demo experiment, it is best to put the O2k into action yourself.

        During lunch breaks, sufficient time is available for relaxing walks and talks, to enjoy the refreshing scenery of the alpine environment, or use the spare time for specific tutorials. With DatLab 4.3 we accomplish data analysis on-line during the experiment, providing final results and their graphical presentation by the end of an experimental run. Thus we gain sufficient time to demonstrate MultiSensor perspectives, see the Titration-Injection microPump TIP-2k with new feedback-control in action and practice its simple and automatic operation.



IOC53 Feedback

  • "Thank you very much for this week of course in Austria." - Arnaud Mourier, Stockholm, Sweden

  • "Thank you for an informative course on use of the Oxygraph-2k and related subjects. It is one of the most interesting courses I have had." - Vinh Than Phung, Norway

  • "I want to thank you again for a very interesting course both scientifically and social. The PC presentation from Diana was fantastic." -  Knut M. Skatvedt, Norway

  • "Thank you again for the wonderful respirometry course in Schrocken!" - Paul Coen, Pittsburgh, USA



Participants IOC53

 



Day 1

Thursday, 30. July

 

16:00 Participants arriving in Bregenz: Meeting point at 4:00 pm in Bregenz train station; 1.1 hour drive to Schröcken. Check in at Hotel Tannberg

18:30                       Welcome reception Hotel Tannberg

19:00                       Dinner

 

21:00           Erich Gnaiger: Beyond respiratory states 1-2-3-4: electron transport system (ETS), OXPHOS capacity and LEAK respiration -  Experimental advances with high-resolution respirometry.



Day 2

Friday, 31. July

Principles of high-resolution respirometry - from switching on the Oxygraph­2k to the experimental result.

 

08:30 – 09:30             The O2k system: Introduction and oxygen calibration of the polarographic oxygen sensors (POS).

09:30 – 10:30             Hands-on: Oxygen sensor calibration with DatLab 4.

10:30                       Coffee break

 

11:00 – 12:00             Erich Gnaiger: Experimental protocols for substrate-uncoupler-inhibitor titrations (SUIT protocols): An introduction.

12:00                     Lunch break; walk and talk.

 

15:00 -16:30             Kathrin Renner and Erich Gnaiger: Demo experiment – the Oxygraph-2k and a SUIT protocol.

16:30                       Coffee break

 

17:30 – 18:45             Hands-on: Experiment with the Oxygraph-2k (5 O2k - 10 chambers): SUIT protocol; on-line DatLab analysis.

19:00                       Dinner

21:00                            Discussion of results and protocol of the demo experiment. 



Day 3

Saturday, 01. August

 

08:30 – 09:00         Mario Fasching:  Introduction - Instrumental Background

09:00 – 12:00             Hands-on: Instrumental background test with the Oxygraph-2k - Washing and filling the O2k chambers with experimental media; air calibration; instrumental background competition, DatLab background analysis.

A. Instrumental background test for expeirments with cells and isolated mitochondria, from air saturation to zero oxygen concentration, wth automatic TIP-2k titration protocol (see #1.3.B. Instrumental background with dithionite and TIP-2k feedback control. MiPNet 14.6).

B. Instrumental background test for experiments with permeabilized muscle fibers, in the high-oxygen range of 500 to 200 µM. Manual titration of hydrogen peroxide into MiR06 (MiR05 with catalase).

12:00                       Lunch break – walk and talk

16:00 – 16:45             Erich Gnaiger: DatLab background analysis – summary.

16:45                       Coffee break

                                      Parallel group sessions:

                                      O2k Setup     POS Service      DatLab Analysis    TIP-2k

17:15 – 18:00             Gr. 1               Gr. 2                   Gr. 3                        Gr. 4

18:00 – 18:45             Gr. 4               Gr. 1                   Gr. 2                        Gr. 3

19:00                       Dinner

21:00                       Hot topics: MiPNet Session (10+10 min) 



Day 4

Sunday, 02. August

                              Parallel group sessions:

                                      O2k Setup     POS Service      DatLab Analysis    TIP-2k

08:30 – 09:15             Gr. 3               Gr. 4                   Gr. 1                        Gr. 2

09:15 – 10:00             Gr. 2               Gr. 3                   Gr. 4                        Gr. 1

10:00                       Coffee break

 

10:30 - 11:30              Mario Fasching: Trouble Shooting

 

12:00                       Walk and welcome at the Alpmuseum uf m Tannberg (www.alpmuseum.at), refreshment in the lake Körebersee or at Hotel Körbersee (we keep the details of timing flexible according to weather conditions).

16:45                       Coffee (Hotel Tannberg)

 

17:15 - 18:45             Mario Fasching: Principles and developments of the O2k-MultiSensorAnalyzer. Demo experiment with a high-resolution TPP electrode.

19:00                      Dinner



Day 5

Monday, 03. August

 

09:30 – 10:30             Erich Gnaiger: Respiratory measurements at low oxygen: Aerobic-anoxic transitios and steady-state control

10:30                       Coffee break

 

11:00 - 12:00              Summary of O2k-features and hands-on with DatLab Analysis:

A.      The O2k and advanced features of DatLab 4.

B.      Practise DatLab 4 with demo data.

12:00                       Lunch break – walk and talk

15:00 - 16:00              The O2k system: Open questions

16:00                       Coffee

 

16:30 - 18:00           Erich Gnaiger: Experimental protocols.

a) Phosphorylation control protocol with intact cells: ROUTINE – LEAK – ETS

b) Diagnostic multiple SUIT protocols with mitochondrial preparations.

18:00 - 18:45              Questions and discussion of protocols, analysis and trouble shooting.

19:00                      Dinner

 

21:00                       Discussion - Summary – Conclusions

                                      Farewell party of IOC53 



Day 6

Tuesday, 04. August

                              Early morning:  Departure


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