Hotel Mohnenfluh, Schroecken, Vorarlberg, Austria

• 4-8 July 2008, 47th IOC - International Course on High-Resolution Respirometry. Hotel Mohnenfluh, Schroecken, Vorarlberg, Austria.

Supported by European Union Project MITOFOOD COST Action Number FA0602; Coordinator: Dr. Jaap Keijer

12-16 July 2008, 47th IOC - International Course on High-Resolution Respirometry. Schroecken, Vorarlberg, Austria.

• Lecturers and tutors
• Programme 
• Contents: Overview on high-resolution respirometry
• Participants

Med. Univ. Innsbruck, Dept. General Transplant Surgery, D. Swarovski Research Lab., Innsbruck, Austria; and OROBOROS INSTRUMENTS, Austria

• Fasching Mario, PhD, mario.fasching@oroboros.at
• Gnaiger Erich, PhD, erich.gnaiger@i-med.ac.at
• Köfler Simone, Mag, simone.koefler@oroboros.at (administration)
• Lemieux Hélène, PhD, helene.lemieux@oroboros.at
• Scandurra Francesca, PhD, francesca.scandurra@oroboros.at
• Subarsky Patrick, PhD, patrick.subarsky@oroboros.at

• O2k-Course Programme[Download pdf 1.7 MB; 2008-07-08]

Saturday, 12. July

18:30       Welcome reception

19:00        Dinner

Day 1 (Sunday, 13. July)

09:00 - 12:45              Joint Session with MiPsummer school (lecture hall)

Mitochondrial respiratory capacity and respiratory control

14:30 - 15:00              Introduction to O2k-Design (seminar room, hotel Mohnenfluh)

15:00 - 17:45              Parallel group sessions 1:

Hands-on experiments with the Oxygraph-2k - instrumental performance: O2k-calibration; Instrumental setup and service.

17:45 - 18:45              Summary of instrumental setup, trouble shooting.

19:00                       Dinner

Day 2 (Monday, 14. July)

09:00 - 12:45             Joint Session wit MiPsummer School (lecture hall)

Membrane potential, coupling, substrates and respiratory control

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14:30 - 15:00             Introduction to Datlab

15:00 – 19:00          Parallel group sessions:

Hands-on with the Oxygraph-2k (five instruments - ten parallel chambers):

Principles of high-resolution respirometry - from switching on the Oxygraph-2k to the experimental result (demo experiment).

·       Oxygraph-2k demo experiment with DatLab 4.3.

·       Oxygen calibration of the polarographic oxygen sensors (POS).

·       Preparation of permeabilized muscle fibers.

·       Determination of fiber wet weight with METTLER TOLEDO microbalance XS205DU (1 to 3 mg per chamber).

·       Addition of muscle fibers, closing the chamber.

·                Demo experiment: Multiple substrate-uncoupler-inhibitor titration (OXPHOS titration protocols) and on-line DatLab analysis.

17:30 -18:45            Special Interest Group: TPP+ / MultiSensor Application with the O2k (parallel to  a 2^nd run of the Demo experiment)

Day 3 (Tuesday, 15. July)

08:30 – 09:00             Introduction: Instrumental Background

09:00 - 12:00              Parallel group sessions:

Hands-on experiments with the Oxygraph-2k - instrumental performance: O2k-calibration; instrumental background competition, DatLab analysis, O2k-manual.

12:00                       MiPsummer Walk to the cheese and wine reception at the Alpmuseum uf m Tannberg (www.alpmuseum.at); refreshment in the lake Körebersee or at Hotel Körbersee.

17:00-17:45                Parallel group sessions: Hands-on with Datlab:  DatLab analysis of Demo Experiment and Background.

18:00–19:00            Demo Experiment MultiSensor (lecture hall)

                              Oxygen and pH: Calibration and rationale of application.

19:00  Dinner

21:15                            Discussion - Summary – Conclusions

Wednesday, 16. July

Departure

or continuation with the MiPsummer School 2008 

 

 

[up]

• Comelli Marina, Dr., Dipartimenti di Scienze e Tecnologie Biomediche, Università degli Studi di Udine, Udine, Italy. - mcomelli@mail.dstb.uniud.it (R05)
• Hawke Emma, PhD Student, Verona University, Verona, Italy. - emma.hawke@crimper.co.nz (R09)
• Hughey Curtis, PhD Student, Department of Biochemistry & Molecular Biology, University of Calgary, Canada. - cchughey@ucalgary.ca (R11)
• Latini Alexandra, Prof.Dr., Center of Biological Sciences UFSC, Universidade Federal de Santa Catarina Campus Universitario Trindede, Florionopolis, Brazil. - alatini@ccb.ufsc.br (R14)
• Lucchinetti Eliana, Dr., Institute of Anesthesiology, E-HOF University Hospital Zurich, Switzerland. - eliana.lucchinetti@usz.ch (R04)
• Milenkovic Dusanka, Institute for Biochemistry and Molecular Biology, Freiburg, Germany. - dusanka.milenkovic@biochemie.uni-freiburg.de (R02)
• Miinalainen Ilkka, PhD, Centre for Bioanalytical Sciences (CBAS), Dublin City University, Dublin, Ireland. - ilkka.miinalainen@dcu.ie (R12)
• Polyak Erzsebet,  Ph.D., Divison of Human Genetics, Children's Hospital of Philadelphia,  Philadelphia, USA. - polyake@email.chop.edu (R10)
• Praturi Gopala Krishna, PhD Student, Centre for cellular and molecular Biology, Hyderabad, India. - praturi@ccmb.res.in (R03)
• Pustovidko Antonina, Dr., A.N. Belozersky Institue of Physiko-Chemical Biology, MSU, Leninskie, Moscow, Russia. - nell_a@mail.ru  (R06)
• Rennison Julie, PhD Student, Rennison Julie, Dept. of Molecular Cardiology, Cleveland Clinic, Cleveland, USA. - julie.rennison@case.edu (R13)
• Sarna Neha, PhD Student, Faculty of Kinesiology, University of Calgary, Canada. – neha_sarna@hotmail.com (R08)
• Stride Nis, Copenhagen Muscle Research Centre, Copenhagen, Denmark. - nis.stride@gmail.com (R07)
• Vögtle Nora, Institute for Biochemistry and Molecular Biology, Freiburg, Germany. - nora.voegtle@biochemie.uni-freiburg.de (R01)