41st International Course on High-Resolution Respirometry


Hotel Mohnenfluh, Schroecken, Vorarlberg, Austria

 

  • 18-22 July 2007, 41st O2k-Course, Schroecken, Austria.

Satellite to the first International MiPsummer School on Mitochondrial Respiratory Physiology (2007 Jul 12-18).



Table of Contents

 

     

 

 



Programme

Details of the program are subject to change according to special interests of participants.

 

Day 1 (Wenesday, 18. July)

Check in at Hotel Mohnenfluh; 19:30 dinner. Before/after dinner: Setting up the Oxygraph-2k with a glass of wine.

 

Day 2 (Thursday, 19. July)

 

08:45 - 11:45 From switching on the Oxygraph-2k to the experimental result (demo experiment).

  • Oxygraph-2k demo experiment with DatLab 4;
  • Oxygen calibration of the polarographic O2 sensor;
  • Addition of cells, closing the chambers;
  • "Phosphorylation control titration": Routine respiration (Cr), oligomycin-induced state (CrO), uncoupled state (Cru); inhibition by rotenone and myxothiazole (CruRM); 
  • FCCP titration with the TIP-2k.

12:00 - 16:00           Lunch break, alpine walks and talks.

16:15 -19:00  Working group session 1

Hands-on with the Oxygraph-2k (four instruments - eight parallel chambers): Cell respiration experiment.

19:30                       Dinner

21:00                       Hot topics in Mitochondrial Physiology. MiPNet Session 1.

 

Day 3 (Friday, 20. July)

 

08:45 - 11:45  Working group session 2

High-resolution respirometry with the O2k and DatLab 4: Calibration of the oxygen signal, instrumental and chemical background flux; Instrumental setup and service.

12:00 - 16:00           Lunch break, alpine walks and talks.

16:15 - 19:00  Working group session 3

High-resolution respirometry and DatLab 4; Instrumental setup and service.

19:30                       Dinner

21:00                      Hot topics in Mitochondrial Physiology: MiPNet Session 2.

 

Day 4 (Saturday, 21. July)

 

08:45 - 12:30  Working group session 4

High-resolution respirometry and DatLab 4; Instrumental setup and service.

13:00 - 17:00          Walk to a welcome at the Alpmuseum uf m Tannberg.

17:30 -19:30  Working group session 5

Special interest groups: TIP-2k, inhibitor titration and flux control / Experimental regimes / Oxygen kinetics.

20:00                       Dinner

Discussion - Summary - Conclusions

 

Day 5 (Sunday, 22. July)

Departure

[up]



Lecturers and Tutors

 

 

  • Gnaiger Erich, Medical University of Innsbruck, Dept. General and Transplant Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria; and OROBOROS INSTRUMENTS, Austria. erich.gnaiger@i-med.ac.at
  • Lemieux Hélène, Medical University of Innsbruck, Dept. General and Transplant Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria; and OROBOROS INSTRUMENTS, Austria. helene.lemieux@oroboros.at
  • Scandurra Francesca, Medical University of Innsbruck, Dept. General and Transplant Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria; and OROBOROS INSTRUMENTS, Austria. francesca.scandurra@oroboros.at

 



Guest lecturers

 

  • Hand Steven C., Department of Biological Sciences, Louisiana State University, 103 Life Sciences Bldg, Baton Rouge, LA 70803, USA; shand@lsu.edu 
  • Hoppel Charles, Case Western Reserve University, Department of Pharmacology, Center for Mitochondrial Diseases, Cleveland, Ohio, USA; charles.hoppel@case.edu



Participants - July 2007

Last update: 2007-06-29

  • Dalmonte Elena, The Faculty of Medicine II, Department of Biochemical Sciences, University of Rome La Sapienza, Roma, Italy. - lenecera@gmail.com (R04)
  • Endlicher René, Department of Physiology, Faculty of Medicine, Charles University, Hradec Kralove, Czech Republic. - endlicherr@lfhk.cuni.cz (R05)
  • Gnaiger Erich, Medical University of Innsbruck, Dept. Transplant Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria; and OROBOROS INSTRUMENTS, Innsbruck, Austria. - erich.gnaiger@oroboros.at (O1)
  • Hand Steven C., Department of Biological Sciences, Louisiana State University, 103 Life Sciences Bldg, Baton Rouge, LA 70803, USA; shand@lsu.edu (invited lecturer)
  • Hoppel Charles, Case Western Reserve University, Department of Pharmacology, Center for Mitochondrial Diseases, Cleveland, Ohio, USA; charles.hoppel@case.edu (invited lecturer)
  • Jilkova Zuzana, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic. - jilkova@biomed.cas.cz (R03)
  • Julian David, University of Florida, Dept. of Zoology, Gainesville, Florida, US. - djulian@ufl.edu (R07)
  • Lemieux Hélène, Medical University of Innsbruck, Dept. Transplant Surgery, D. Swarovski Research Laboratory, Innsbruck, Austria. - helene.lemieux@oroboros.at (O3)
  • Meissner Maxi, Laboratory of Pediatrics, Liver and Digestive Diseases
    University Hospital Groningen, NL. - m.meissner@med.umcg.nl (R01)
  • Muntean Danina, Dept. Pathopysiology, University of Medicine and Pharmacy, Timisoara, Romania. - daninamuntean@gmail.com (R09).
  • Nuskova Hana, Dept. of Bioenergetics, Institute of Physiology,
    Academy of Sciences of the Czech Rep. Prague 4, Czech Republic. (R08)
  • Scandurra Francesca M., The Faculty of Medicine II, Department of Biochemical Sciences, University of Rome La Sapienza, Roma, Italy. -  francescascandurra@yahoo.it (O2)
  • Votyakova Tatyana V, Diabetes Institute, Rangos Research Center, Pittsburgh, PA, USA. - tav2+@pitt.edu (R02)
  • Wohlgemuth Stephanie, University of Florida, College of Medicine,
    Department of Aging and Geriatrics, University of Florida, Gainesville Florida, US. - swohlgemuth@aging.ufl.edu (R06)

 


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