O2k-Workshop: Recommended Reading
NEW: Pesta D, Gnaiger E (2010) High-Resolution Respirometry. OXPHOS protocols for human cell cultures and permeabilized fibres from small biopisies of human muscle. In: Mitochondrial bioenergetics: methods and protocols (Series Editor: Sir John Walker), edited by Carlos Palmeira and Antσnio Moreno. In press.
Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352. A methodological introduction into high-resolution respirometr, with focus on:
Polarographic oxygen sensor and traditional oxygraphy High-resolution respirometry: The Oxygraph-2k Calibration of Polarographic Oxygen Sensors and Oxygen Concentration in Respiration Media at Air Saturation From Oxygraph Slopes to Respiratory Flux Corrected for Background Effects Phosphorylation control protocol with intact cells Titration Steps of the PC Protocol Experimental Example for the PC Protocol Flux Control Ratios from the PC Protocol Intact cells, permeabilized cells and tissue, or isolated mitochondria?
Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. Int. J. Biochem. Cell Biol. 41: 18371845.
· Respirometry with permeabilized fibres and isolated mitochondria; · Convergent CI+II electron input and OXPHOS capacity; · Tissue-OXPHOS capacity in human permeabilized muscle fibres and isolated mitochondria; · Tissue-OXPHOS capacity and functional diversity
Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir. Physiol. 128: 277-297. A detailed introduction into high-resolution respirrometry with particular emphasis on kinetics and measurements at low oxygen:
Mitochondrial kinetics measured by high-resolution respirometry Calibrations and corrections for response time and instrumental background Steady-state injection respirometry Mitochondrial respiratory control at low oxygen Apparent oxygen affinity and catalytic efficiency of mitochondrial respiration Effect of ADP and oxygen limitation on ADP/O2 flux ratios The low-oxygen environment of the cell: Mitochondria between hypoxic and oxidative stress
Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York: 431-442. Isolated mitochondria and permeabilized muscle fibers, MiR05:
Optimization of mitochondrial cold storage Mitochondrial respiration medium, MiR05 Mitochondrial cold ischemia-reperfusion injury
Renner K, Amberger A, Konwalinka G, Kofler R, Gnaiger E (2003) Changes of mitochondrial respiration, mitochondrial content and cell size after induction of apoptosis in leukemia cells. Biochim. Biophys. Acta 1642: 115-123.
Intact cells cytochrome c oxidase cytochrome c test, respiration per million cells, per citrate synthase, per mg protein, or per cytochrome c oxidase activity.
Gnaiger E (2009) Mitochondrial Pathways through Complexes I+II: Convergent Electron Transport at the Q-Junction and Additive Effect of Substrate Combinations. In: Mitochondrial Pathways and Respiratory Control. OROBOROS MiPNet Publications 2009, 2nd ed.
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