1

Wednesday, December 05

Arrival

15:00                               

Arrival in Bregenz: Meeting point Bregenz train station at 3:00 pm; approx. 1 hour bus drive to Schröcken and Hochtannberg (Salober).  Transfer/walk to Hotel Körbersee

18:30             

Welcome reception at Hotel Körbersee.

19:00

Dinner

20:30-21:00            

Get-together: Introduction of participants and their research interests

2

Thursday, December 06

Workshop 1

Weblink

07:30-08:30

Breakfast

08:00

Organize loan of skiing equipment

O2k-Core

Principles of high-resolution respirometry - from switching on the Oxygraph-2k to the experimental result

Gnaiger 2008 POS

08:30-09:00

Erich Gnaiger: Get O2k-Connected with your OROBOROS-USB:
a guided tour to the O2k

get O2k-Connected

09:00-10:00

Instrumental quality control 1:
The oxygen sensor OroboPOS -  calibration, stability testing, and evaluation of sensitivity to measure oxygen flux.

O2k-Calibration

10:00

Organize special lunch or bad weather task groups  (fibre and homogenate prep, fluorometry and spectrophotometry, NO, pH and Ca, Bioblast wiki login) and loan of skiing equipment

11:00

Practice - skiing, walk & talk, or individual O2k-tasks  /  Lunch

14:00

Coffee / Tea

14:30-16:00

O2k-Demo experiment 1:
Respiration of intact cells and on-line DatLab Analysis: Simultaneous measurement of oxygen consumption (O2k-Core) and H₂O₂ production (add-on O2k-Fluorescence LED2-Module)

O2k-Fluorometry Workshop

16:00

Coffee / Tea

16:30-17:15

DatLab Guide through the menues:
DL-Demo files and DL-Excel templates

DatLab Guide

17:15-18:00

Experimental design 1:  Coupling control of mitochondrial respiration: LEAK, OXPHOS, ETS, ROX

DatLab Flux Analysis

18:30

Dinner

20:00-21:00

MiPNet-Lectures by guest tutors
Votion DM (BE): OXPHOS protocols for the study of training and rare myopathies in horse skeletal muscle fibres.
Shilpa Iyer (US): Respiration in intact cells.

3

Friday, December 07

Workshop 2

Weblink

07:30

Breakfast

O2k-Basic

O2k instrumental setup                OroboPOS service

O2k-Start

08:30-09:15

Groups A and B     Groups C and D

POS Service

09:15-10:00

Groups C and D     Groups A and B

10:00

Practice - skiing, walk & talk, or individual O2k-tasks  /  Lunch

14:00

Coffee / Tea

14:30-16:00

Instrumental quality control 2:
O2k-Background test and on-line analysis of oxygen flux.

O2k-Background

16:00

Coffee / Tea

16:30-18:30

Hands-on (6 groups): O2k-Background from air saturation to zero oxygen concentration; or for permeabilized muscle fibres in the high-oxygen range of 500 - 200 µM.
O2k-Background with automatic TIP2k or manual titrations.

O2k-Background

18:30

Dinner

20:00-21:00

Hot MiP topics 10 + 5 min
Rodrigues MF (BR), Towheed MA (US), Konrad C (HU), Kiss G (HU)

IOC72 Abstracts
MiPNet17.13

4

Saturday, December 08

Workshop 3

Weblink

07:30

Breakfast

08:30-09:30

MiPNet-Lectures by guest tutors
Chuck Hoppel (US): OXPHOS protocols for diagnosis of mitochondrial diseases with isolated mitochondria.
Steve Hand (US): OXPHOS protocols for the study of mitochondrial function in active and dormant states.

09:30-10:00

DatLab O₂ flux analysis:
Flux per volume, flux per mass, flow per cell, flux control ratio

Glossary: Respiratory states

10:00

Practice - skiing, walk & talk, or individual O2k-tasks  /  Lunch

14:00

Coffee / Tea

14:30-16.00

O2k-Demo experiment 2: SUIT protocol with mouse permeabilized fibres and on-line DatLab analysis

Pesta 2012 Methods Mol Biol

16:00

Coffee / Tea

16:30-17:30

Hands-on: SUIT experiment continued with DatLab Analysis and guide through Excel templates

DatLab Flux Analysis

17:30-18:00

Experimental design 2: Coupling Control Protocol with intact cells: ROUTINE, LEAK, ETS, ROX

Cells: PCP

18:00-18:30

Experimental design 3:
Substrate and coupling control of mitochondrial respiration.

CI+II

18:30

Dinner

20:00-21:00

Hot MiP topics 10 + 5 min
Dzialowski EM (US), Horscroft J (UK), Irving BA (US), Kamath VG (US)

IOC72 Abstracts
MiPNet17.13

5

Sunday, December 09

Workshop 4

Weblink

07:30

Breakfast

08:30-09:15

Working groups: Elaborate answers to the 'Questions for the O2k-Workshop'

IOC-Questions

09:15-09:45

IOC-Questions - discussion of 'Answers'

09:45-10:15

The O2k-Workshop continues with the Bioblast wiki

www.bioblast.at

10:15

Coffee / Tea

10:45-12:00

O2k-Demo experiment 3:  Respiration and steady-state feedback control of oxygen levels with the TIP2k.

TIP2k User Manual

12:00

Lunch

13:00-13:45

Mona Fontana-Ayoub: Introduction into trouble shooting

14:00

Walk (snowshoe rental) to the Alpmuseum: Guided tour and reception: 15 €

www.alpmuseum.at

17:30

Coffee / Tea

18:00-19:00

Erich Gnaiger: MitoPathways through Complexes I+II - perspectives of comparative mitochondrial physiology

Gnaiger 2009 Int J Biochem Cell Biol

19:00

Dinner

20:30-21:00

Panel Discussion - Feedback IOC72

O2k-Feedback - Bioblast

Farewell party

6

Monday, December 10

Departure

Breakfast

Early morning: Departure

Name

Lab  

Ali Sameh S

Center for Aging and Associated Diseases, Helmy Institute of Medical Sciences, IBBE, Mitochondria, synaptosomes, cardiomyocytes, oxidative stress - ET_Giza_Ali SS

Amoedo Nivea Dias

Federal University of Rio de Janeiro, Medical Biochemistry Institute, Mitochondria, cells, oxygen consumption, membrane potential, proton efflux - BR_Rio de Janeiro_Rumjanek FD

Atlante Anna

Consiglio Nazionale delle Ricerche, IBBE, Mitochondria, cells, oxygen consumption, membrane potential, proton efflux - IT_Bari_Atlante A

Bikman Benjamin T

Brigham Young University, Sphingolipids, Insulin resistance - US_UT Provo_Bikman BT

Bresciani Martins de Andrade Paula

Institute of Physical Activity and Sport Sciences, Cruzeiro do Sul University, White adipose tissue, fatty acids, mitochondria - BR_Sao Paulo_BM de Andrade P

Burtscher Johannes
(team tutor)

Department of Pharmacology, Medical Uni Innsbruck - AT_Innsbruck_Gnaiger E 

Colleoni Francesca

Department of Physiology, Development & Neuroscience, University of Cambridge, Placenta, hypoxia, bioenergetics, metabolism, pregnancy - UK_Cambridge_Murray AJ

Dudzinska Dominika

Department of Haemostasis and Haemostatic Disorders, Medical University of Lodz - Polyphenols, neutrophils, respiratory burst - PL_Lodz_Watala C

Dzialowski Edward M

Department of Biological Sciences, University of North Texas - Endothermy, avian, reptile, cardiac, skeletal, liver, hypoxia - US_TX Denton_Dzialowski EM

Fontana-Ayoub Mona
(team tutor)

AT_Innsbruck_OROBOROS INSTRUMENTS

Friederich Marisa

University of Colorado, Respiratory chain enzyme analysis, mitochondrial diseases - US_CO Denver_Van Hove J

Gnaiger Erich
(organizer, tutor)

DSL, Dept Visceral, Transpl Thoracic Surgery, Medical Univ Innsbruck - AT_Innsbruck_OROBOROS INSTRUMENTS

Goy Sarah

Department of Biological Sciences, University of North Texas, Endothermy, avian, reptile, cardiac, skeletal, liver, hypoxia - US_TX Denton_Dzialowski EM

Hand Steven
(guest lecturer)

Louisiana State University - US_LA Baton Rouge_Hand SC

Hoppel Charles
(guest lecturer)

Case Western Reserve University School of Medicine
 - US_OH Cleveland_Hoppel CL

Horscroft James

Department of Physiology, Development and Neuroscience, University of Cambridge, Heart, cardiac metabolism, Angiotensin II, mitochondria - UK_Cambridge_Murray AJ

Irving Brain

Obesity Institute Center for Nutrition and Weight Management, Aging, diabetes, NASH, NAFLD, exercise - US_PA Danville_Irving BA

Iyer Shilpa
(guest tutor)

Virginia Commonwealth University - US_VA Richmond_Iyer S

Kamath Vasudeva Gurupur

College of Medicine, Central Michigan University, Nucleoside, NrtI - US_MI Mt Pleasant_McKee E

Karlsson Michael

Mitochondrial Pathophysiology Unit, University Lund, Malaria, sepsis, multiple organ failure - SE_Lund_Elmer E

Kiss Gergely
(guest tutor)

Semmelweis University - HU_Budapest_Chinopoulos C

Konrad Csaba
(guest tutor)

Semmelweis University - HU_Budapest_Chinopoulos C

Lai Nicola

Case Western Reserve University School of Medicine, Department of Pharmacology, Center for Mitochondrial Diseases, Oxygen transport and energy metabolism, skeletal muscle mitochondrial function in Type 2 Diabetes - US_OH Cleveland_Hoppel CL

Le Catherine
(guest tutor)

Colorado State University Fort Collins -
US_CO Fort Collins_Chicco AJ

Leo Elettra

Alfred Wegener Institute for Polar and Marine Research, Mitochondrial complexes, temperature, pH, membrane potential, ATP turnover - DE_Bremerhaven_Mark FC

Leonhardt Andrea

Sanofi-Aventis Deutschland GmbH, Respirometry, ATP production, skeletal muscle function, mitochondrial biogenesis, sarcopenia and muscle atrophy - DE_Frankfurt_Leeuw T

Lundby Stine

Institute of Physiology, University Zuerich, Skeletal muscle mitochondrial function - CH_Zurich_Lundby C

Petrosillo Guiseppe

Department of Biochemistry and Molecular Biology, University Bari, Mitochondria, cells, oxygen consumption, membrane potential, proton efflux - IT_Bari_Atlante A

Pidcoke Heather

US Army Institute of Surgical Research, Mitochondrial impact on platelet biology and function, US_TX Houston_Cap A

Rodrigues Figueiredo Mariana

Federal University of Rio de Janeiro, Medical Biochemistry Institute, Sodium butyrate, mitochondrial function, glycolysis - BR_Rio de Janeiro_Rumjanek FD

Romestaing Caroline

Laboratoire de Physiologie Intégrative, Cellulaire et Moléculaire, Mitochondria in ecophysiology, energy allocation, oxidative stress, ROS, ATP, fitness - FR_Villeurbanne_Romestaing C

Senyilmaz Deniz

German Cancer Research Center (DKFZ), Signal transduction in cancer and metabolism - DE_Heidelberg_Teleman A

Shahini Albana

NeuroVive, Pharmaceuticals AB, Mitochondrial pathophysiology - SE_Lund_Elmer E

Sirsat Tushar

Department of Biological Sciences, University of North Texas, Endothermy, avian, reptile, cardiac, skeletal, liver, hypoxia - US_TX Denton_Dzialowski EM

Sparks Lauren

Burnham Institute for Medical Research, Muscle mitochondria, fatty acids, human cells - US_FL Orlando_Smith SR

Surampudi Vasudha

Center for the Study of Biological Complexity, Virginia Commonwealth University, Respirometric studies in stem cells - US_VA Richmond_Iyer S

Towheed Mohammad Atif

Pittsburgh Institute for Neurodegenerative Diseases (PIND), University of Pittsburgh, Mitochondrial encephalomyopathies, Drosophila, ATP6, ATP synthase, OXPHOS - US_PA Pittsburgh_Palladino MJ

Tsygankova Polina

Lab metab. Disorders, Research centre for medical genetics, Pdh-deficiency, Alpers syndrome, Leigh syndrome, mitochondrial DNA depletion syndromes - RU_Moscow_Tsygankova P

Votion Dominique-Marie (guest lecturer)

University of Liège - BE_Liege_Votion DM

Wohlwend Martin

K.G. Jebsen Centre of Exercise in Medicine, Norwegian University of Science and Technology (NTNU), Exercise, mitochondria, aging - NO_Trondheim_Rognmo O