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Oxygraph-2k: DatLab Software
Highway from data to information
DatLab is the program for Data Acquisition and Analysis, specifically developed for high-resolution respirometry: On-line display of oxygen concentration and oxygen flux and many other special features. Most of the performance of DatLab runs in the background, no longer troubling the experimenter. This provides speed and reliability, including automatic instrumental or chemical background correction, expression of respiration in selected units (oxygen flux per volume, per mg protein or mg biopsy, per million cells), and immediate numerical output of average flux for specifically marked sections of the experiment.
The figure shows the on-line display with DatLab 4 of an experiment with intact cells. The blue line is oxygen concentration* O2-Calibration-Solubility, the red line is respiration* O2 Concentration and Flux (per million cells). Respiration was measured initially in the ROUTINE state, then inhibited by a manual titration of oligomycin, followed by an automatic step-titration of the uncoupler FCCP with the Titration-Injection microPump, TIP2k, to obtain the non-coupled state (ETS capacity). See MiPNet 10.4 for further experimental information.
DatLab 4.3 - New Features
DatLab- Templates for DatLab Analysis:
HistorySeveral new new features are incorporated into the software version DatLab 4.3:
Graph layout: Graph layout setups can be viewed and selected (task list: Layout), and edited or deleted (Modify). Oxygen and pX calibration: The calibration status is indicated for each chamber by a window with colour code. Oxygen calibration window: For improved quality control, the equilibrium oxygen concentration is displayed at calibration, and signal stability is shown as obtained during the calibration interval. Calibration parameters can be imported from another DatLab file. O2k-MultiSensor calibration: In a user-friendly calibration window, a two-point calibration is made, and the pX signal can be displayed as calibrated plot or as raw signal. Calibration parameters (Oxygraph \ O2 Calibration) are copied to clipboard, for instertion into a new Excel template file ‘O2k-Calibration_Clipboard.xls’. A track record is thus conveniently obtained for the function of each sensor, monitoring signal stability of the POS over several months without exchange of membranes and electrolyte. File \ Export -> Data to Textfile: You can export all or selected plots for further applications in Windows™ pograms (Excel, SigmaPlot, etc.). File \ Export -> Events: A list of events, including event time and comments, is exported. Experiment \ Edit [F3]: “Flux derivation N” Select various degrees of smoothing for calculation of flux, to improve either time resolution or to decrease noise. Templates can be saved and applied in the window Experiment \ Edit [F3], for (1) the sample and medium, and (2) parameters for background correction of flux. Plots \ Flux Control Ratios: For an entire experiment, flux can be normalized for a reference flux (maximum) and a correction for a baseline flux (e.g. non-mitochondrial respiration). The resulting plot of Flux Control Ratios (FCR) provide a concept for normalization superior to the conventional respiratory control ratio (see MiPNet Protocols: FCR). Default mark names: Marks are labelled with consecutive numbers, starting from 01, independent of the sequence of position of the marks. When deleting a mark [Shift+R8] other than the highest number, then this mark number is missing on the mark list. For any number <99, the consecutive automatic mark name will be the next higher number. >99, all marks are named as 00. Extending the default mark names, e.g. from ‘03’ to ’03-TD’, maintains the numerical sequence for default names in subsequently generated marks. Marks \ Statistics [F2]: Sort by ‘Mark name’ or ‘Time’. You can select the sequence of marks to be listed in alphanumerical order (Mark name) or in a time sequence (Time). Marks \ Name: Names for the active plot are listed and can be edited in this overview window. These mark names can be saved as a template, for automatically renaming all marks of another plot in any experiment. All marks can also be renamed in numerical sequence. Graph -> Display Numerical Values: The current numerical value is displayed on-line in the graph, for one plot on the Y1 and a second plot on the Y2 axis. Graph \ Options: More options are available for the graphs, such as font size, event line style, selectively show/not show events and marks. Events: The line style has changed. Events for both chambers are now navy blue (previously: red), for the right chamber only the event-lines are green. Events: The time of the event is displayed in the ‘Edit Event’ window in the formats hh:mm:ss and in seconds. - TIP-2k -> Feedback-Control: The entirely new feature allows feedback control of the performance of the TIP-2k.
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