OROBOROS celebrates the 80th O2k-Workshop

  ****                      the 30th IOC in Schroecken                   ****



IOC80. International O2k-Workshop on HRR and O2k-Fluorometry

 

2013 October 09 - 14

Schroecken, Hotel Koerbersee, Austria - IOC80 is the 30th IOC in Schroecken



This workshop is limited to 30 participants using 6 Oxygraph-2k (fully booked).

>> New: Training for scientific expeditions



Programme IOC80

 

 

1

Wednesday, Oct 09

*printed in workshop materials

 

Arrival

Weblink

15:00 

Arrival in Bregenz: Meeting point Bregenz train station at 3:00 pm; approx. 1 hour bus drive to Schröcken and Hochtannberg (Salober).  Transfer/walk to Hotel Körbersee

IOC-travel

18:30

Welcome reception at Hotel Körbersee

Schroecken

19:00

Dinner

 

20:30-21:00

Get-together: introduction of participants and their research interests - a welcome by OROBOROS INSTRUMENTS

IOC80*

2

Thursday, Oct 10

 

Workshop 1

Weblink

07:30-08:30

Breakfast

 

 

Principles of high-resolution respirometry and O2k-Fluorometry - from switching on the Oxygraph-2k to the experimental result

Gnaiger 2008 POS*

08:30-09:15

Get O2k-Connected with OROBOROS: a guided tour to the Oxygraph-2k

get O2k-Connected

09:15-10:00

Introduction to a DemoExperiment with the O2k

Pesta 2012 Methods Mol Biol*

10:00

Coffee / Tea

 

10:30-12:00

O2k-Demo experiment 1: Respiration of intact cells and on-line DatLab Analysis: Simultaneous measurement of oxygen consumption (O2k-Core) and H2O2 production (O2k-Fluorescence LED2-Module)

MiPNet18.06_Amplex-Yeast*

12:00

Lunch - Walk & Talk

 

15:00-16:00

O2k instrumental setup and sensor service - overview

O2k-Manual

16:00

Coffee / Tea

 

 

O2k instrumental setup               OroboPOS service

 

16:30-17:15

Groups 1-5                                      Groups 6-10

O2k-Start

17:15-18:00

Groups 6-10                                    Groups 1-5

POS Service

18:30

Dinner

 

20:00-21:00

Protocol design: mt-prep - tissues, limitations imposed by fluorometry - controls parallel to fluorometry

MiPNet18.05 Amplex-Mouse-heart

3

Friday, Oct 11

 

Workshop 2

Weblink

07:30-08:30

Breakfast

 

08:30-09:15

Experimental design 1: Coupling control protocol with intact cells: ROUTINE, LEAK, ETS, ROX

Cells: PCP

09:15-10:00

Experimental design 2: Substrate and coupling control of mitochondrial respiration - MitoPathways through CI+II

The Blue Book
pp 29-43
*

10:00

Coffee / Tea

 

10:30-12:00

SUIT protocol with DatLab Analysis and guide through Excel templates

DatLab Flux Analysis

12:00

Lunch - Fishing

The Blue Book
p 56
*

15:00-16:00

Tissue homogenate preparation: The PBI-Shredder

MiPNet17.03 Shredder vs Fibres*

16:00

Coffee / Tea

 

16:30-18:00

O2k-Demo experiment 2: Respiration with tissue homogenate: SUIT protocol, oxygen regime, washing

Krumschnabel 2013 Abstract MiP2013: 26-27*

18:30

Dinner

 

20:00-21:00

Hot MiP-Topics: 10+5 min presentations of abstracts by participants

IOC80 Abstracts
MiPNet18.09
*

 

4

Saturday, Oct 12

 

Workshop 3

Weblink

07:30-08:30

Breakfast

 

08:30-09:15

DatLab O2 flux analysis: Flux per volume, flux per mass, flow per cell, flux control ratio, flux control factor

Glossary: Respiratory states

09:15-10:00

DatLab Guide through the menus:
DL-Demo files and DL-Excel templates

DatLab Guide

10:00

Coffee / Tea

 

10:30-12:00

DatLab Analysis: hands-on in 10 teams

DatLab Flux Analysis

12:00

Lunch - Walk & Talk

 

15:00-16:00

Instrumental quality control 1:
The oxygen sensor OroboPOS -  calibration, stability testing, and evaluation of sensitivity to measure oxygen flux.

O2k-Calibration

16:00

Coffee / Tea

MiPNet18.10 O2kvsMultiwell*

16:30-17:15

Instrumental quality control 2:
O2k-Background test and on-line analysis of oxygen flux.

O2k-Background*

17:15-18:00

Hands-on (6 groups): O2k-Background from air saturation to zero oxygen concentration; or for permeabilized muscle fibres in the high-oxygen range of 500 - 200 µM.
O2k-Background with automatic TIP2k or manual titrations.

O2k-Background*

18:30

Dinner

 

20:00-21:00

10x5-min team presentations:  DatLab Analysis: results, what did we learn, what was difficult, open questions?

 

5

Sunday, Oct 13

 

Workshop 4

Weblink

07:30-08:30

Breakfast

 

08:30-09:15

O2k-MultiSensor overview and O2k-Fluorometry applications: Amplex™ red and safranin

MiPNet17.17_Amplex-Mouse-brain*

09:15-10:00

OXPHOS analysis: A challenge for the simultaneous measurement of respiration and mt-membrane potential

 

10:00

Coffee / Tea

 

10:30-12:00

O2k-Demo experiment 3:  Respiration and steady-state feedback control of oxygen levels with the TIP2k.

TIP2k User Manual

12:00

Lunch packages

 

12:30-15:30

Walk to the Alpmuseum: Guided tour and reception: 15 €

www.alpmuseum.at*

16:00

Coffee / Tea

 

16:00-16:45

Working groups: Elaborate answers to the 'Questions for the O2k-Workshop'

IOC-Questions*

16:45-17:15

IOC-Questions - discussion of 'Answers'

 

17:15-18:00

Introduction to trouble shooting

O2k-Troubleshooting

18:00-18:45

The O2k-Workshop continues with the Bioblast wiki
- in the spirit of Gentle Science

www.bioblast.at

19:00

Dinner

 

20:30-21:00

Panel Discussion - Feedback IOC80

O2k-Feedback

 

Farewell party

 

6

Monday, Oct 14

 

Departure / Fish project

 

 

Breakfast

 

 

Early morning: Departure / Fish heart and liver

Gnaiger 1993 Verh Dtsch Zool Ges*

 

 



Lecturers and Tutors

OROBOROS O2k-Team

Gnaiger Erich, Ao.Univ.-Prof., PhD

Laner Verena, MSc

Fontana-Ayoub Mona, BMA

Krumschnabel Gerhard, Univ. Doz. PhD



Participants

Name

Lab 

Bagur Quetglas Rafaela

FR_Grenoble_Schlattner U: Laboratoire de Bioénergétique Fondamentale et Appliquée, Université Joseph Fourier, Grenoble. - metabolic control analysis, respiration regulation, respiratory chain complexes activities. 

Becker Christina

DE_Cologne_Trifunovic A: Institute of Genetics, University of Cologne.

Consitt Leslie

US_OH Athens_Consitt L: Biomedical Sciences, Ohio University. - Diabetes, obesity, aging, skeletal muscle.

Feng Jianhua

CH_Zurich_Zaugg K:  Universitätsklinik für
Radio-Onkologie, Inselspital, Bern. - p53, mitochondria, CPTIC

Fernandez-Vizarra Erika

UK_Cambridge_Zeviani M: MRC Mitochondrial Biology Unit - Cambridge. - Mitochondrial Respiratory Chain assembly disorders, Complex I, Complex III, Complex IV.

Ferrando Miguel Rosa

FR_Paris_Bigou S: Hôpital de la Pitié-Salpêtrière, Paris. - Respirometry in cell culture and animal models in Parkinson's disease

Fontana-Ayoub Mona (tutor)

AT_Innsbruck_OROBOROS INSTRUMENTS

Garcia-Souza Luiz Felipe

BR_Rio de Janeiro_Oliveira MF: Institute of Medical Biochemistry, Federal University of Rio de Janeiro. - platelet, thrombin, activation

Gnaiger Erich
(lecturer)

AT_Innsbruck_OROBOROS INSTRUMENTS: Mitochondrial Physiology Laboratory, Dept Visceral, Transpl Thoracic Surgery, Medical University Innsbruck.

Garrabou Gloria

ES_Barcelona_Moren C: Muscle Research and Mitochondrial Function Laboratory, University of Barcelona.

Goy Christine

DE_Duesseldorf_Haendeler J: IUF-Leibniz Research Institute for Environmental Medicine/Molecular Aging Research. - Mitochondrial Telomerase Reverse Transcriptase, mitochondrial functions

Jung Ki-Duck

KR_Seoul_Mymed: Mymed Co. Ltd, Seoul.

Kobayashi Hirosuke

JP_Kanagawa_Kobayashi H: Dept. of med. Engineering and Technology, Kitasato University School of Allied Health Sciences.

Kokubo Kenichi

JP_Kanagawa_Kobayashi H: Dept. of med. Engineering and Technology, Kitasato University School of Allied Health Sciences.

Krumschnabel Gerhard (tutor)

AT_Innsbruck_OROBOROS INSTRUMENTS

Krylova Tatiana

RU_Moscow_Zakharova E: Research centre for medical genetics, RAMS, Moscow. - Point mutations of mitochondrial DNA.

Kwak Hyo-Bum

KR_Incheon_Kwak HB: Dept. of Kinesiology, Inha University, Incheon.

Laner Verena (tutor)

AT_Innsbruck_OROBOROS INSTRUMENTS

Michalak Slawomir

PL_Poznan_Michalak S: Department of Neurochemistry and Neuropathology, Poznan University. - neurological paraneoplastic syndromes, multiple sclerosis, Parkinson's disease, peripheral blood mononuclear cells

Mäkelä Kari

FI_Oulo_Mäkelä K: Institute of Biomedicine/Physiology
University of Oulu. - brown adipose tissue, white adipose tissue

Meszaros Andras

HU_Szeged_Boros M: Institute of Surgical Research, School of Medicine, University of Szeged. - hypoxia, reoxygenation, electrophyl methyl groups (EMGs), methane, long-chain fatty acids

Mukhina Irina

RU_Nizhny Novgorod_Mukhina I: Central Research Laboratory, Nizhny Novgorod State Medical Academy. - Cell respiration, hypoxia, oxidative stress, Ischemia-reperfusion injury, mitochondrial pathways, respiratory  control

Salin Karine

UK_Glasgow_Metcalfe N: Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow. - fish, oxidative stress, environmental response, coupling.

Scheidnagl Simone

AT_Innsbruck_Gnaiger E: Institute of Zoology, University of Innsbruck

Schreiber Renate

AT_Graz_Zechner R: Institut für Molekulare Biowissenschaften -
Universität Graz. - Adipose tissue

Shelchkova Natalia

RU_Nizhny Novgorod_Mukhina I: Central Research Laboratory, Nizhny Novgorod State Medical Academy. - Cell respiration, hypoxia, oxidative stress, Ischemia-reperfusion injury, mitochondrial pathways, respiratory  control

Siddall Charles Parker

US_IN Indianapolis_Brozinick JT: Eli Lilly and Company, Lilly Corporate Center.

Stephens Natalie

US_FL Orlando_Sparks LM: Translational Research Institute for Metabolism and Diabetes, Orlando.

Van Heck-Kappen Antonia

NL_Nijmegen_Rodenburg R: Department Laboratory Medicine,
Radboud University Nijmegen Medical Centre. - Diagnostics of Human mitochondrial disorders in skeletal muscle and fibroblasts by measuring ATP production, oxidation rates and enzyme activity from respiratory chain

Willmes Diana

DE_Berlin_Birkenfeld AL: Center for Cardiovascular Research (CCR), Department of Endocrinology, Diabetes and Nutrition, Charité Berlin. - metabolism, hepatocytes, obesity.

Wintjes Liesbeth

NL_Nijmegen_Rodenburg R: Department Laboratory Medicine,
Radboud University Nijmegen Medical Centre. - Diagnostics of Human mitochondrial disorders in skeletal muscle and fibroblasts by measuring ATP production, oxidation rates and enzyme activity from respiratory chain

Wuest Rob

NL_Amsterdam_Wuest R: Department of Physiology
VU Medical Center. - cardiomyocytes, diabetes, heart failure, ROS

Volodina Maria

RU_Moscow_Skulachev VP: Research Center for Obstetrics, Gynecology and Perinatology, Moscow. - mitochondria, oxidative stress, pregnancy disorders, fertility




 

OROBOROS O2k-Workshops 

are listed as MitoGlobal Events



Training for scientific expeditions

 

A two-day training for scientific expeditions is offered following IOC80 (14th-15th October) at the same location: Heart and liver mitochondria of Arctic charr will be studied from fish grown in the alpine lake Koerbersee (altidue 1750 m) and compared with rainbow trout mitochondria.

 

A Power-O2k approach (6 instruments, 12 chambers) and fast tissue-homogenate preparation with the PBI-Shredder will provide the basis for a statistically complete study.

 


The O2k-Workshop on HRR and O2k-Fluorometry is a component of the K-Regio project MitoCom Tyrol.


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