O2k-Fluorescence LED2-Module - Prototype



IOC66 O2k-Fluorometry Workshop

 

Innsbruck, Austria; 2012 March 15–16

  • Programme, participants, demo experiment: MiPNet17.06  - download pdf

        >>> IOC66-Feedback

 



Outlook to OPEN INNOVATION


  • O2k-Fluorescence LED2-Module
  • O2k-Fluorescence traces - hydrogenperoxide with resolution in the nanomolar range: MiP-Poster_O2k-Fluorescence
  • Hands-on workshop
  • Lectures and discussion on protocols for the measurement of hydrogen peroxide production (Amplex red), mt-membrane potential (Safranin), ATP production (Mg green), and Ca2+ (Ca green).
  • Short presentations of 'Hot topics in Mitochondrial Physiology' .. and take a look at the MitoPedia-Glossary - 50 years ago

Registration and Accomodation

  • Registration Form download pdf

    Registration (IOC66): 550.- € Regular, 350.- Students. Registration fee waived in combination with the purchase of your O2k-Fluorescence Module; free registration for partners of MitoCom Tyrol.

  • Accommodation - We recommend Hotel Mozart: Bed and breakfast, in walking distance to the workshop venue and to the medieval centre of Innsbruck. For booking, contact: hotel.mozart@gmail.com



High-resolution terminology - Bioblast wiki

         Fluori/ometry

  • Invitation
  • Time: 2012 March 15 - 16 (Welcome on March 14, MiPArt-Gallery, OROBOROS INSTRUMENTS, Schöpstr. 18, Innsbruck)
  • Lecture hall: Medical University Innsbruck, Austria (Lecture hall 4, Schöpfstr 41, Innsbruck)
  • IOC66-locations: City map
  • Organized in the frame of MitoCom Tyrol.





North Chain - here was the IOC66-farewell dinner - www.nordkette.com/en/top/home.html - www.oroboros.at






View from the OROBOROS Office to the 'North Chain' - Seegrube and Hafelekar




IOC66 Programme

  

 

Wednesday, March 14, 2012 - Arrival

 

 

15:15 -

Pre-workshop workshop: test experiments with the O2k - quality control

18:18 -

Welcome reception at OROBOROS INSTRUMENTS - MiPArt Gallery, Schoepfstr. 18, Innsbruck
* www.mipart.at

 

 

 

Thursday, March 15, 2012

 

 

 

Workshop: Medical University Innsbruck, Austria - Lecture hall 4, Schöpfstr 41

09:00-09:30

Erich Gnaiger - Innsbruck, AT

 

Development of the O2k-Fluorometer: Synergies of MitoCom Tyrol and international user-innovation.

09:30-10:15

Laszlo Tretter - Budapest, HU

 

Measurement of ROS production in isolated mitochondria. The influence of membrane potential and substrate oxidation.

10:15-10:45

Coffee / Tea

10:45-11.45

Mario Fasching - Innsbruck, AT

 

Optimization of the O2k-Fluorescence LED2-Module: Hardware considerations - wavelenghths and geometry. 

 

Demo experments with Amplex red: Signal and chemical stability, effect of mitochondrial media and light intensity.

11:45-12:15

Discussion

12:15-14:30

Lunch: Restaurant Glasmalerei - Müllerstrasse 10, corner Glasmalereistrasse (group reservation)

14:30-15:15

O2k-Team

 

First Demo experiment with the O2k-Fluorescence LED2-Module and O2k-Multisensor System.

15:15-15:35

Andrea Eigentler - Innsbruck, AT

 

Tissue homogenate preparation with the PBI-Shredder for optical measurements: Evaluation of mitochondrial respiration in homogenate versus permeabilized fibres from mouse myocardium.

15:35-16:00

Coffee / Tea

16:00-16:45

Second Demo experiment

 

Hot topics in Mitochondrial Physiology

16:45-17:30

Anthony Hickey - Auckland, NZ

 

Hydrogen peroxide production and respiration measured in the O2k: Comparative mitochondrial physiology.

17:30-17:45

Paul Coen - Pittsburgh, US

 

Skeletal muscle mitochondrial energetics are associated with maximal aerobic capacity and walking speed in older adults.

17:45-18:00

Pablo Garcia-Roves - Barcelona, ES

 

Tissue-specific control of mitochondrial respiration in obesity-related insulin resistance and diabetes

18:00-18:15

John Boyle - Leeds, UK

 

Regional skeletal muscle remodeling and mitochondrial dysfunction in right ventricular heart failure.

19:00

Dinner: Restaurant Ottoburg, Friedrichstube - Herzog-Friedrich-Straße 1 (group reservation)

21:30

Continue in Filou - with music by Archie and Friends - Stiftgasse 12 - www.filou.cc

 

Friday, March 16, 2012 

 

 

 

Workshop: Medical University Innsbruck, Austria - Lecture hall 4, Schöpfstr 41

09:00-09:30

Erich Gnaiger - Innsbruck, AT

 

O2k-Fluorometry: Open innovation @Bioblast

09:30-10:15

Christos Chinopoulos - Budapest, HU

 

A fluorometric kinetic assay of mitochondrial ADP-ATP exchange mediated by the ANT in isolated mitochondria and permeabilized cells.

10:15-10:45

Coffee / Tea

 

Hot topics in Mitochondrial Physiology

10:45-11.00

Csaba Konrad - Budapest, HU

 

Absence of Ca2+-induced mitochondrial permeability transition but presence of bongkrekate-sensitive nucleotide exchange in C. crangon and P. serratus.

11:00-11:15

Gergely Kiss - Budapest, HU

 

Reduction in the activity of alpha-KGDHC prompts respiration-impaired mitochondria towards extramitochondrial ATP consumption.

11:15-11:30

Karl Johan Tronstad - Bergen, NO

 

Modulation of mitochondrial energy metabolism leads to respiratory dysfunction and metabolic stress via cell-specific pathways in leukemia cells.

11:30-12:15

Discussion on protocols for fluorometric measurements of H2O2 production, mt-membrane potential, ATP production, and further perspectives of application of fluorescent dyes.

12:30-14:00

Lunch at OROBOROS INSTRUMENTS - MiPArt Gallery, Schoepfstr. 18, Innsbruck
* www.mipart.at

14:00-15:15

Hands-on experiments with the O2k-Fluorescence LED2-Module and O2k-Multisensor System - I.

15:15-15:45

Coffee / Tea

15:45-17:00

Hands-on experiments with the O2k-Fluorescence LED2-Module and O2k-Multisensor System – II.

17:00-17:20

David Harrison - St. Lorenzen, IT

 

Development of O2k-Spectrophotometry

17:20-18:00

Data analysis - Discussion - Feedback - Conclusions

18:00-18:30

Walk to Innsbruck-station of cable-car. The journey begins at 560 m above sea level.

18:30-19:30

Ascent by cable car Hungerburg  from Innsbruck to Hungerburg, continued by Panorama cable car to 1905-meter high Seegrube (but this time not further on to Hafelekar at 2256 m) - Conclusions with a bird's eye view ..

19:30 -

Dinner at Alpenlounge Seegrube-Restaurant (last descent at 23:30). On March 08, the snowcover on the mountain reached about 2.8 m.



IOC66 Participants, Lecturers and Tutors

John Boyle - lecturer

Department of Cardiovascular & Neuronal Remodelling, Leeds Institute for Genetics, Health and Therapeutics (LIGHT), Leeds

UK

Christos Chinopoulos - guest lecturer

Department of Medical Biochemistry, Semmelweis University, Budapest

HU

Paul Coen - lecturer

Department of Health and Physical Activity, University of Pittsburgh, Pittsburgh

US

Konrad Csaba - student presentation

Department of Medical Biochemistry, Semmelweis University, Budapest

HU

Andrea Eigentler - staff, scientific support

D. Swarovski Research Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University of Innsbruck

AT

Mario Fasching - staff, scientific support

OROBOROS INSTRUMENTS, Innsbruck

AT

Michael Fischer

Institute for Orthopaedics, Medical University of Innsbruck

AT

Mona Fontana-Ayoub - staff, scientific support

OROBOROS INSTRUMENTS, Innsbruck

AT

Pablo M Garcia-Roves - lecturer

Diabetes and Obesity Laboratory, Institut d'Investigacions Biomediques August Pi Sunyer (IDIBAPS), Hospital Clinic de Barcelona, Barcelona

ES

Kiss Gergely - student presentation

Department of Medical Biochemistry, Semmelweis University, Budapest

HU

Erich Gnaiger - staff, scientific support

D. Swarovski Research Laboratory, Dept Visceral, Transpl Thoracic Surgery, Medical University of Innsbruck; OROBOROS INSTRUMENTS, Innsbruck

AT

Vera Goossens

VIB-Ugent, Gent

BE

Naïg Gueguen

Département de Biochimie et Génétique, Ingénieur hospitalier Biochimie Métabolique et Hormonologie, Angers

FR

Lukas Gradl - DatLab 5

sofware security networks, Innsbruck

AT

Mary-Ellen Harper

Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa

CA

David K Harrison - staff, scientific support

St. Lorenzen

IT

Anthony Hickey - guest lecturer (staff)

School of Biological Sciences, University of Auckland , Auckland

NZ

Victor Jeger

Department of Intensive Care Medicine (KIM), Universitätsspital Bern

CH

Barbara Meissner - staff, scientific support

OROBOROS INSTRUMENTS, Innsbruck

AT

Dominik Pesta - scientific support

Medical University of Innsbruck

AT

Oivind Rognmo

Faculty of Medicine, ISB, Norwegian University of Science and Technology (NTNU), Trondheim

NO

Lydia Staudacher - staff, administration

Medical University of Innsbruck

AT

Katrin Stecher - staff, administration

OROBOROS INSTRUMENTS, Innsbruck

AT

Katharina Stelzl - staff, administration

OROBOROS INSTRUMENTS, Innsbruck

AT

Laszlo Tretter - guest lecturer

Department of Medical Biochemistry, Semmelweis University, Budapest

HU

Karl Johan Tronstad - lecturer

Department of Biomedicine, University of Bergen, Bergen

NO

Claudio Zoppi

Departament of Anatomy, Cellular Biology and Physiology and Biophysics, Campinas

BR

 

 




 

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